14‐3‐3 proteins interact with a 13‐lipoxygenase, but not with a 9‐lipoxygenase

Holtman, W.; Roberts, Michael R.; Oppedijk, B.; Testerink, Christa; Zeijl, Mieke J. van; Wang, Mei

doi:10.1016/s0014-5793(00)01575-1

Cited by 17 publications

(13 citation statements)

References 32 publications

(50 reference statements)

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“…As demonstrated before, proinflammatory LTB 4 like PALMs are generated from linolenic and linoic acid both by autoxidation and enzyme activity of lipoxygenases [27]. Notably, the 14-3-3 protein, which was upregulated in urban pollen, interacts with lipoxygenases in other species [47, 48]. In general 14-3-3 proteins are 30-kDa, phosphoserine/phosphothreonine-binding proteins.…”

Section: Discussionmentioning

confidence: 95%

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

Bryce¹,

Drews

Schenk

et al. 2009

Int Arch Allergy Immunol

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Background: Epidemiologic studies reveal a dramatic increase in allergies in the last decades. Air pollution is considered to be one of the factors responsible for this augmentation. The aim of this study was to analyze the impact of urbanization on birch pollen. The birch pollen proteome was investigated in order to identify differences in protein abundance between pollen from rural and urban areas. The allergenicity of birch pollen from both areas was evaluated by assessing its chemotactic potency as well as its protein and allergen contents. Methods: Difference gel electrophoresis (DIGE) was used to analyze the pollen proteome. The chemotactic activity of aqueous pollen extracts was determined by migration assays of human neutrophils. Results: DIGE revealed 26 differences in protein spot intensity between pollen from urban and rural areas. One of these proteins was identified by de novo sequencing as the 14-3-3 protein, which resembles a stress-induced factor in other plant species. Furthermore, extracts from pollen collected in urban areas had higher chemotactic activity on human neutrophils compared to pollen from rural sites. Conclusions: The present study points to an impact of air pollution on allergen carrier proteome and release of chemotactic substances. The increment in proinflammatory substances such as pollen-associated lipid mediators might contribute to the described urban-rural gradient of allergy prevalence. Furthermore, our study suggests that allergenicity is determined by more than the sole allergen content.

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Section: Discussionmentioning

confidence: 95%

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

Bryce¹,

Drews

Schenk

et al. 2009

Int Arch Allergy Immunol

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“…Six mammalian isoforms can all bind Bad in vitro and are able to reduce the apoptotic effects of Bad on cells (Subramanian et al, 2001). In plants studies, three different Arabidopsis isoforms were able to activate the H-ATPase (Baunsgaard et al, 1998) and surface plasmon resonance has shown that three barley isoforms can bind a lipoxygenase enzyme with similar affinities (Holtman et al, 2000). While not exhaustive in their treatments across isoforms, these studies and other studies indicate that at the biochemical interaction level, there can be overlap and similarity among isoforms with regard to client affinities.…”

Section: Elements Of the Issuementioning

confidence: 99%

14-3-3 phosphoprotein interaction networks – does isoform diversity present functional interaction specification?

Paul¹,

Denison²,

Schultz³

et al. 2012

Front. Plant Sci.

108

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The 14-3-3 proteins have emerged as major phosphoprotein interaction proteins and thereby constitute a key node in the Arabidopsis Interactome Map, a node through which a large number of important signals pass. Throughout their history of discovery and description, the 14-3-3s have been described as protein families and there has been some evidence that the different 14-3-3 family members within any organism might carry isoform-specific functions. However, there has also been evidence for redundancy of 14-3-3 function, suggesting that the perceived 14-3-3 diversity may be the accumulation of neutral mutations over evolutionary time and as some 14-3-3 genes develop tissue or organ-specific expression. This situation has led to a currently unresolved question – does 14-3-3 isoform sequence diversity indicate functional diversity at the biochemical or cellular level? We discuss here some of the key observations on both sides of the resulting debate, and present a set of contrastable observations to address the theory functional diversity does exist among 14-3-3 isoforms. The resulting model suggests strongly that there are indeed functional specificities in the 14-3-3s of Arabidopsis. The model further suggests that 14-3-3 diversity and specificity should enter into the discussion of 14-3-3 roles in signal transduction and be directly approached in 14-3-3 experimentation. It is hoped that future studies involving 14-3-3s will continue to address specificity in experimental design and analysis.

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“…Five of the proteins identified here have been previously shown to be 14-3-3 binding proteins in other plant species. These are: sucrose phosphate synthase [5,6]; O-methyl transferase [36]; lipoxygenase 2 [37]; nitrate reductase [12,13]; and glyceraldehyde-3-phosphate dehydrogenase [11,38]. Thus 49 (91%) of the reported 14-3-3 interactions in Table 1 are novel.…”

Section: Specificity Of Bindingmentioning

confidence: 99%

A proteomic analysis of 14‐3‐3 binding proteins from developing barley grains

Alexander

Morris

2006

Proteomics

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14-3-3 proteins are important eukaryotic regulatory proteins. Barley (Hordeum vulgare L.) 14-3-3A was over-expressed, immobilised and used to affinity purify 14-3-3 binding proteins from developing barley grains. Binding was shown to be phosphorylation-dependent. These proteins were fractionated by PAGE and identified by MALDI-TOF MS. In total, 54 14-3-3 binding proteins were identified, 49 of these interactions are novel to plants. These proteins fell into a number of functional categories. The largest category was for carbohydrate metabolism, including plastidic enzymes for starch synthesis and modification. 14-3-3 was shown to be present in isolated plastids. Four of five enzymes involved in sucrose biosynthesis from triose phosphates were identified, suggesting co-ordinated regulation of this pathway. Invertase and sucrose synthase, which break down sucrose to hexoses, were found. Sucrose synthase activity was shown to be inhibited by exogenous 14-3-3 in a dosage-dependent manner. The second-largest functional group was for proteins involved in stress and defence responses; for example, RGH2A, closely related to the MLA powdery mildew resistance protein, was found. This work illustrates the broad range of processes in which 14-3-3 may be involved, and augments previous data demonstrating key roles in carbohydrate metabolism and plant defence.

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14‐3‐3 proteins interact with a 13‐lipoxygenase, but not with a 9‐lipoxygenase

Cited by 17 publications

References 32 publications

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

14-3-3 phosphoprotein interaction networks – does isoform diversity present functional interaction specification?

A proteomic analysis of 14‐3‐3 binding proteins from developing barley grains

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