14-3-3 Proteins and a 13-lipoxygenase form associations in a phosphorylation-dependent manner

Holtman, W.; Roberts, Michael R.; Wang, Mei

doi:10.1042/bst0280834

Cited by 9 publications

(2 citation statements)

References 1 publication

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“…As demonstrated before, proinflammatory LTB 4 like PALMs are generated from linolenic and linoic acid both by autoxidation and enzyme activity of lipoxygenases [27]. Notably, the 14-3-3 protein, which was upregulated in urban pollen, interacts with lipoxygenases in other species [47, 48]. In general 14-3-3 proteins are 30-kDa, phosphoserine/phosphothreonine-binding proteins.…”

Section: Discussionmentioning

confidence: 99%

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

Bryce¹,

Drews

Schenk

et al. 2009

Int Arch Allergy Immunol

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Background: Epidemiologic studies reveal a dramatic increase in allergies in the last decades. Air pollution is considered to be one of the factors responsible for this augmentation. The aim of this study was to analyze the impact of urbanization on birch pollen. The birch pollen proteome was investigated in order to identify differences in protein abundance between pollen from rural and urban areas. The allergenicity of birch pollen from both areas was evaluated by assessing its chemotactic potency as well as its protein and allergen contents. Methods: Difference gel electrophoresis (DIGE) was used to analyze the pollen proteome. The chemotactic activity of aqueous pollen extracts was determined by migration assays of human neutrophils. Results: DIGE revealed 26 differences in protein spot intensity between pollen from urban and rural areas. One of these proteins was identified by de novo sequencing as the 14-3-3 protein, which resembles a stress-induced factor in other plant species. Furthermore, extracts from pollen collected in urban areas had higher chemotactic activity on human neutrophils compared to pollen from rural sites. Conclusions: The present study points to an impact of air pollution on allergen carrier proteome and release of chemotactic substances. The increment in proinflammatory substances such as pollen-associated lipid mediators might contribute to the described urban-rural gradient of allergy prevalence. Furthermore, our study suggests that allergenicity is determined by more than the sole allergen content.

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Section: Discussionmentioning

confidence: 99%

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

Bryce¹,

Drews

Schenk

et al. 2009

Int Arch Allergy Immunol

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“…The products of LOX activity also exert a direct deterrent function towards pathogens (León et al 2002). LOXs (EC 1.13.11.12) are key enzymes in lipid metabolism and catalyse the first step in oxygenation of polyunsaturated fatty acids (Holtman et al. 2000).…”

Section: Introductionmentioning

confidence: 99%

Correlation of cytological and biochemical parameters with resistance and tolerance to Mycosphaerella graminicola in wheat

et al. 2011

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This study investigated the infection process of Mycosphaerella graminicola and enzyme activities related to reactive oxygen species (ROS) or oxylipin biosynthesis in four French wheat cultivars with variable resistance to M. graminicola infection. At field level, cultivars Caphorn, Maxyl and Gen11 were susceptible, whereas Capnor showed high levels of quantitative resistance. Moreover, Capnor and Gen11 were tolerant, i.e., their yield was less affected by infection compared to non-tolerant Maxyl and Caphorn. These four cultivars were inoculated under laboratory conditions with the M. graminicola IPO323 reference strain. Cytological and biochemical responses were studied on collected first plantlet leaves and several features discriminated between cultivars. However, resistance and tolerance had no impact on the fungal infection process. Levels of lipoxygenase (LOX), peroxidase (PO) and glutathione-S-transferase (GST) activities were also compared with regard to cultivar resistance or tolerance to M. graminicola. LOX, PO and GST activities did not discriminate resistance and tolerance profiles, although a low level of PO in inoculated and non-inoculated plants could be associated with tolerance. In addition, cell necrosis correlated positively with LOX in non-tolerant cultivars, while mycelia surrounding stomata were positively correlated with PO in the resistant cultivar. GST activity presented correlations between cytological and biochemical parameters only for susceptible cultivars. Stomatal and direct penetration were positively correlated with GST activity in the susceptible non-tolerant cultivars, while these correlations were negative in the tolerant cultivar. When combining cytological and biochemical observations with resistance and tolerance profiles, for each cultivar and at each time point, cultivars could be classified in tight accordance with their previous field characterisation. Moreover, tolerance allowed us to distinguish susceptible cultivars when both biochemical and cytological parameters were considered together.

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14‐3‐3 Protein: Effectors of Enzyme Function

Sehnke

Ferl

2018

Annual Plant Reviews Online

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The sections in this article areIntroductionStructure and Biochemical Characterization of 14‐3‐3 ProteinsPlant 14‐3‐3 Protein Families and OrganizationMode of Action of 14‐3‐3 Proteins14‐3‐3 Proteins and the Regulation of Metabolic Enzymes14‐3‐3 Proteins and the Regulation of Non‐Metabolic EnzymesIsoform Preference for 14‐3‐3 Target ProteinsLocalization of Plant 14‐3‐3 sPotential 14‐3‐3 Binding Partners in PlantsConclusionAcknowledgements

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14-3-3 Proteins and a 13-lipoxygenase form associations in a phosphorylation-dependent manner

Cited by 9 publications

References 1 publication

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

Impact of Urbanization on the Proteome of Birch Pollen and Its Chemotactic Activity on Human Granulocytes

Correlation of cytological and biochemical parameters with resistance and tolerance to Mycosphaerella graminicola in wheat

14‐3‐3 Protein: Effectors of Enzyme Function

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