14-3-3 gamma is required to enforce both the incomplete S phase and G2 DNA damage checkpoints

Hosing, Amol S.; Kundu, Samrat T.; Dalal, Sorab N.

doi:10.4161/cc.7.20.6812

Cited by 31 publications

(20 citation statements)

References 34 publications

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“…Two isoforms of 14-3-3 family members, ɛ and γ, specifically form heterodimers and interact with cdc25 to regulate the cell cycle [22,23]. Furthermore, 14-3-3 proteins are associated with many types of human cancers.…”

Section: Discussionmentioning

confidence: 99%

Involvement of 14-3-3γ overexpression in extrahepatic metastasis of hepatocellular carcinoma

Lai

Chang

et al. 2011

Human Pathology

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Section: Discussionmentioning

confidence: 99%

Involvement of 14-3-3γ overexpression in extrahepatic metastasis of hepatocellular carcinoma

Lai

Chang

et al. 2011

Human Pathology

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“…As a matter of fact, upon overexpression of MEF2C, we observed an induction of 14-3-3g, Gadd45b and p21 transcripts, encoding inhibitors of the CYCLIN B1/CDK1 kinase. 24,[52][53][54][55][56] These genes have a role in the G2-M checkpoint both in normal cell cycle progression and in response to DNA damage. We also found that failure of MEF2C degradation correlates with an increase in the cytoplasmic inactive fraction of CYCLIN B1.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation-dependent degradation of MEF2C contributes to regulate G2/M transition

et al. 2015

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The Myocyte Enhancer Factor 2C (MEF2C) transcription factor plays a critical role in skeletal muscle differentiation, promoting muscle-specific gene transcription. Here we report that in proliferating cells MEF2C is degraded in mitosis by the Anaphase Promoting Complex/Cyclosome (APC/C) and that this downregulation is necessary for an efficient progression of the cell cycle. We show that this mechanism of degradation requires the presence on MEF2C of a D-box (R-X-X-L) and 2 phospho-motifs, pSer98 and pSer110. Both the D-box and pSer110 motifs are encoded by the ubiquitous alternate a1 exon. These two domains mediate the interaction between MEF2C and CDC20, a co-activator of APC/C. We further report that in myoblasts, MEF2C regulates the expression of G2/M checkpoint genes (14-3-3g, Gadd45b and p21) and the sub-cellular localization of CYCLIN B1. The importance of controlling MEF2C levels during the cell cycle is reinforced by the observation that modulation of its expression affects the proliferation rate of colon cancer cells. Our findings show that beside the well-established role as pro-myogenic transcription factor, MEF2C can also function as a regulator of cell proliferation.

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“…To identify the mechanisms that lead to a decrease in cell-cell adhesion, we used a HCT116 cell line model in which 14-3-3c had been knocked down (sh14-3-3c) (described previously by Hosing et al, 2008). 14-3-3c mRNA and protein levels were lower in the sh14-3-3c cells in comparison with the control cells ( Fig.…”

Section: Loss Of 14-3-3c Leads To Sterility In Male Micementioning

confidence: 99%

14-3-3γ meditated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo

Sehgal

Mukhopadhyay

Rajan

et al. 2014

Journal of Cell Science

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The regulation of cell-cell adhesion is important for the processes of tissue formation and morphogenesis. Here we report that loss of 14-3-3γ leads to a decrease in cell-cell adhesion and a defect in the transport of plakoglobin (PG) and other desmosomal proteins to the cell border in HCT116 cells and in the mouse testis. 14-3-3γ binds to PG in a PKCμ dependent fashion resulting in microtubule dependent transport of PG to the border. Transport of PG to the border is dependent on the KIF5B/KLC1 complex. Knockdown of KIF5B in HCT116 cells or in the mouse testis, results in a phenotype similar to that observed with 14-3-3γ knockdown. Our results suggest that loss of 14-3-3γ leads to decreased desmosome formation and a decrease in cell-cell adhesion in vitro and in vivo in the mouse testis leading to defects in testis organization and spermatogenesis.

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14-3-3 gamma is required to enforce both the incomplete S phase and G2 DNA damage checkpoints

Cited by 31 publications

References 34 publications

Involvement of 14-3-3γ overexpression in extrahepatic metastasis of hepatocellular carcinoma

Involvement of 14-3-3γ overexpression in extrahepatic metastasis of hepatocellular carcinoma

Phosphorylation-dependent degradation of MEF2C contributes to regulate G2/M transition

14-3-3γ meditated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo

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