14‐3‐3 epsilon plays an important role in testicular germ cell apoptosis: A functional proteomic study of experimental varicocele

He, Qing; Wu, Xiao; Liu, Xiao peng; Yang, Xiangwei; Zhong, Yuan; Zhang, Y.

doi:10.1111/and.13275

Cited by 5 publications

(4 citation statements)

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“…Their roles in spermatogenesis, normal sperm function, and male fertility have been reported in mice. Such observations imply that dysregulation in proteins involved in apoptotic mechanisms may lead to infertility . Two chaperonin-related protein-coded genes, CCT7 and CCT2, were overexpressed in COVID-19-recovered patients.…”

Section: Discussionmentioning

confidence: 99%

“…Such observations imply that dysregulation in proteins involved in apoptotic mechanisms may lead to infertility. 39 Two chaperonin-related protein-coded genes, CCT7 and CCT2, were overexpressed in COVID-19-recovered patients. These are the vital subunit proteins of the molecular chaperone complex CCT/TRiC (chaperonin containing the T-complex/TCP1-ring complex) present in haploid germ cells.…”

Section: Acsmentioning

confidence: 98%

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Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function

et al. 2022

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A considerable section of males suffered from COVID-19, with many experiencing long-term repercussions. Recovered males have been documented to have compromised fertility, albeit the mechanisms remain unclear. We investigated the impact of COVID-19 on semen proteome following complete clinical recovery using mass spectrometry. A label-free quantitative proteomics study involved 10 healthy fertile subjects and 17 COVID-19-recovered men. With 1% false discovery rate and >1 unique peptide stringency, MaxQuant analysis found 1099 proteins and 8503 peptides. Of the 48 differentially expressed proteins between the healthy and COVID-19-recovered groups, 21 proteins were downregulated and 27 were upregulated in COVID-19-recovered males. The major pathways involved in reproductive functions, such as sperm−oocyte recognition, testosterone response, cell motility regulation, adhesion regulation, extracellular matrix adhesion, and endopeptidase activity, were downregulated in COVID-19-recovered patients according to bioinformatics analysis. Furthermore, the targeted approach revealed significant downregulation of semenogelin 1 and prosaposin, two proteins related to male fertility. Therefore, we demonstrate the alteration of semen proteome in response to COVID-19, thus disrupting the male reproductive function despite the patient's clinical remission. Hence, to understand fertility-related biological processes triggered by this infection, a protracted evaluation of the consequences of COVID-19 in recovered men is warranted.

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Section: Discussionmentioning

confidence: 99%

Section: Acsmentioning

confidence: 98%

Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function

et al. 2022

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“…45 More studies have found that members of the Bcl-2 protein family play an important role in 14-3-3-mediated cell proliferation and apoptosis. [46][47][48] As one of the pro-apoptotic proteins of the Bcl-2 family, BAD is phosphorylated by Akt, and 14-3-3 then binds to the phosphorylated BAD to induce conformational changes in BAD and promote the separation of BAD from Bcl-2/Bcl-XL, thereby blocking the precursor effect of BAD apoptosis. [49][50][51] Furthermore, the 14-3-3 protein can bind to the apoptosis-regulating protein BAX of the Bcl-2 family, which prevents BAX from entering the mitochondria and thus terminating the regulatory effect of BAX on apoptosis.…”

Section: Figurementioning

confidence: 99%

<p>miR-31-5p Regulates <em>14-3-3 ɛ</em> to Inhibit Prostate Cancer 22RV1 Cell Survival and Proliferation via PI3K/AKT/Bcl-2 Signaling Pathway</p>

Zhao

Duan

et al. 2020

CMAR

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Introduction: Prostate cancer (PCa) is one of the most common malignancies, and almost all patients with advanced PCa will develop castration-resistant prostate cancer (CRPC) after receiving endocrine therapy. Effective treatment for patients with CRPC has not been established. Novel approaches are needed to identify therapeutic targets for CRPC. Purpose: Recent research studies have found that members of the 14-3-3 family play an important role in the development and progression of PCa. Previous results have shown that 14-3-3 ɛ is significantly upregulated in several cancers. This study aimed to identify novel miRNAs that regulate 14-3-3 ɛ expression and therapeutic targets for CRPC. Methods: In this study, we used computation and experimental approaches for the prediction and verification of the miRNAs targeting 14-3-3 ɛ, and investigated the potential roles of 14-3-3 ɛ in the survival and proliferation of 22RV1 cells. Results: We confirm that mir-31-5p is downregulated in 22RV1 cells and acts as a tumor suppressor by regulating 14-3-3 ɛ. Ectopic expression of miR-31-5p or 14-3-3 ɛ interference significantly inhibits cell proliferation, invasion, and migration in 22RV1 cells, as well as promotes cell apoptosis via the PI3K/AKT/Bcl-2 signaling pathway. Moreover, 14-3-3 ɛ is required for the miR-31-5p-mediated upregulation of the PI3K/AKT/Bcl-2 signaling pathway. Conclusion: Our findings provide information on the underlying mechanisms of miR-31-5p/14-3-3 ɛ in 22RV1 cell proliferation and apoptosis through the PI3K/AKT/Bcl-2 signaling pathway. These results suggest that miR-31-5p and 14-3-3 ɛ may potentially be utilized as novel prognostic markers and therapeutic targets for PCa treatment.

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“…Testicular germ cells and Sertoli cells have been shown to express the 14-3-3 theta isoform [41]. Apoptosis of rat testicular germ cells, induced by experimental varicocele, is controlled by 14-3-3 epsilon which regulates Bax and Bcl-2 expression in vitro [42].…”

Section: The 14-3-3 In Testesmentioning

confidence: 99%

The 14-3-3 (YWHA) Proteins in Mammalian Reproduction

2020

Int. Ann. Sci.

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The 14-3-3 (YWHA or Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation protein) is an acidic and homologous protein family involved in regulation of key biological events including cell cycle, signal transduction and development. They are highly conserved and are expressed ubiquitously across a wide variety of species ranging from plants to animals. Seven isoforms of 14-3-3 exist in mammals, which are encoded by separate genes, have tissue-specific, developmental stage-specific as well as isoform-specific presence and consequences. It is known that these proteins play significant roles in mitosis of mammalian cells and meiosis of amphibians. However, their effects on development and functioning of mammalian gonads and germ cells are not entirely delineated. This paper, for the first time, provides a comprehensive review of the comparative expression, localization/distribution, binding interactions, and importance of the 14-3-3 proteins in gametogenesis and reproduction among multiple female and male mammalian species. Thorough understanding of these proteins in oogenesis and spermatogenesis would help elucidate the physiological basis of fertility in mammals, including humans.

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14‐3‐3 epsilon plays an important role in testicular germ cell apoptosis: A functional proteomic study of experimental varicocele

Cited by 5 publications

References 37 publications

Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function

Semen Proteomics of COVID-19 Convalescent Men Reveals Disruption of Key Biological Pathways Relevant to Male Reproductive Function

<p>miR-31-5p Regulates <em>14-3-3 ɛ</em> to Inhibit Prostate Cancer 22RV1 Cell Survival and Proliferation via PI3K/AKT/Bcl-2 Signaling Pathway</p>

The 14-3-3 (YWHA) Proteins in Mammalian Reproduction

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