13C NMR investigation of phospholipid membranes with the aid of shift reagents

Shapiro, Yu. E.; Viktorov, A. V.; Volkova, V. I.; Barsukov, L.I.; Bystrov, V. F.; Bergelson, L.D.

doi:10.1016/0009-3084(75)90003-1

Cited by 31 publications

(6 citation statements)

References 10 publications

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“…The Gmi peak at 174.76 ppm " With respect to Me4$i at 0.00 ppm. b Assignments of Shapiro et al (1975).…”

Section: Resultsmentioning

confidence: 99%

“…The large range of 13C chemical shifts encountered makes this nucleus ideal for unraveling the spectra of large complex molecules like ganglioside Gmi, which may contain 50 or more different types of carbon atoms. Furthermore, a large body of data has been accumulated on monosaccharides (Bundle et al, 1973;Dorman & Roberts, 1970;Walker et al, 1976), oligosaccharides (Colson & King, 1976;Colson et al, 1974; Dorman & Roberts, 1971), and polysaccharides (Bhattacharjee et al, 1975;Bundle et al, 1974), as well as phospholipids (Shapiro et al, 1975) and sphingolipids; such data form a basis for the interpretation of carbon-13 NMR spectral information in terms of structural features.…”

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confidence: 99%

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Assignment of the carbon-13 nuclear magnetic resonance spectrum of aqueous ganglioside GM1 micelles

Sillerud¹,

Prestegard²,

Yu³

et al. 1978

Biochemistry

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This article describes the natural-abundance Fourier-transform carbon-13 nuclear magnetic resonance spectrum, at 67.88 MHz, of aqueous micelles of bovine brain ganglioside GM1 of purity greater than 99%. Assignments are given for every carbon nucleus in the molecule, on the basis of a comprehensive study of the relevant mono-, di-, tri-, and polysaccharides, including several containing sialic acid (5-acetamido-3, 5-dideoxy-D-glycero-D-galacto-nonulopyranosonic acid), and phospho-, sphingo-, and glycosphingolipids. These assignments represent an extension of the 13C nuclear magnetic resonance data from monosaccharides and lipids to complex oligosaccharides and glycolipids. They also form the basis for interpretation of spectral perturbations induced in GM1 by titration with paramagnetic europium(III). The single sialic acid in GM1 was found to be alpha-glycosidically linked in the oligosaccharide from considerations of its unique anomeric chemical shift. The sialic acid carboxyl and glyceryl side chain, along with additional ligands donated by the 2-acetamido-2-deoxy-beta-D-galactopyranoside and terminal beta-D-galactopyranoside residues in the oligosaccharide portion of GM1, were found to be intimately involved with cation binding. It is proposed that the higher affinity, compared with monomeric sialic acid, of GM1 for cations may result from these additional oligosaccharide groups, which may effectively compete for water ligands in the metal cation coordination sphere.

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“…The Gmi peak at 174.76 ppm " With respect to Me4$i at 0.00 ppm. b Assignments of Shapiro et al (1975).…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Assignment of the carbon-13 nuclear magnetic resonance spectrum of aqueous ganglioside GM1 micelles

Sillerud¹,

Prestegard²,

Yu³

et al. 1978

Biochemistry

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“…Cations such as Pr3+, Yb3+, and Mn2+ bind to the phosphate groups of phospholipid molecules in bilayers and serum lipoproteins and affect both nearby 13C and the 31P phosphate group resonances [e.g., see Shapiro et al (1975), Sears et al (1976), andHenderson et al (1975)]. The cholesterol molecules in the core of HDL particles and lipid hydrocarbon chain methylene groups are too far removed from the cation binding site to be influenced (Table II, Figure 2B).…”

Section: Microenvironments Of Cholesterol Molecules In Hdlmentioning

confidence: 99%

Packing of cholesterol molecules in human high-density lipoproteins

Lund‐Katz¹,

Phillips²

1984

Biochemistry

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“…Probably the most successful applications have been obtained from shift measurements. In vesicle systems the use of shift reagents makes it possible to distinguish between the outside and the inside (Bergelson et al 1970;Bystrov et al 1972;Shapiro et al 1975). This elegant technique provides not only a simple method for studying outside/inside ratios of lipid molecules and the preferential location of one lipid on either side in a mixed lipid system but also transport properties.…”

Section: A Shift Measurementsmentioning

confidence: 99%

Biological and model membranes studied by nuclear magnetic resonance of spin one half nuclei

Wennerström

Lindblom

1977

Quart. Rev. Biophys.

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There is an inherent anisotropy in biological and model membrane systems and this anisotropy has a profound influence on the nuclear magnetic resonance spectra of such systems. For nuclei with a quadrupole moment the quadrupole coupling usually dominates the NMR spectrum, while for nuclei with spin quantum number I = ½ dipolar couplings provides the most important effects. The quadrupole coupling only affects isolated spins and usually gives rise to simple spectra. The dipole interactions on the other hand couple several spins. In a lipid bilayer with a multitude of spin-½ nuclei these couplings can give rise to complicated manybody effects. Thus the interpretation of the NMR spectra of membrane systems poses problems but at the same time there is a lot of information to be gained.

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13C NMR investigation of phospholipid membranes with the aid of shift reagents

Cited by 31 publications

References 10 publications

Assignment of the carbon-13 nuclear magnetic resonance spectrum of aqueous ganglioside GM1 micelles

Assignment of the carbon-13 nuclear magnetic resonance spectrum of aqueous ganglioside GM1 micelles

Packing of cholesterol molecules in human high-density lipoproteins

Biological and model membranes studied by nuclear magnetic resonance of spin one half nuclei

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