[13] Fluorescence resonance energy transfer

Selvin, Paul R.

doi:10.1016/0076-6879(95)46015-2

Cited by 569 publications

(459 citation statements)

References 69 publications

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“…The lanthanides display decay times on the µs/ms timescale, much longer than the ns timescale of most biological autofluorescence [19]. Subsequently, we have seen lanthanides used for high-sensitivity gated detection in immunoassays [20,21] and in DNA hybridization assays [22,23]. The enhanced spectral properties of europium tetracycline described in this paper, suggest that by using metallic particles, even greater sensitivity may potentially be achieved in these sensing platforms.…”

Section: Introductionmentioning

confidence: 77%

Enhanced Lanthanide Luminescence Using Silver Nanostructures: Opportunities for a New Class of Probes with Exceptional Spectral Characteristics

Lakowicz

Geddes

2005

J Fluoresc

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The photophysical effects of europium tetracycline immobilized in thin polyvinyl alcohol films coated onto silver nanostructures have been investigated. Complimentary to recent reports from our laboratories that the close proximity of luorophores to silver nanostructures can enhance their intrinsic radiative decay rate, we show that up to a 16-fold enhancement in lanthanide luminescence is possible, accompanied by a notable reduction in luminescence lifetime. These results suggest the potential future development of a new class of significantly brighter lanthanide based probes with exceptional spectral properties, which can probably undergo significantly more excitation-emission event cycles due to the reduced lifetime, substantially increasing detectability.

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Section: Introductionmentioning

confidence: 77%

Enhanced Lanthanide Luminescence Using Silver Nanostructures: Opportunities for a New Class of Probes with Exceptional Spectral Characteristics

Lakowicz

Geddes

2005

J Fluoresc

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“…An alternative approach is to take advantage of the phenomenon of Förster resonance energy transfer (FRET) [5]. This involves monitoring the transfer of energy from an excited fluorescent molecule (donor) to another molecule (acceptor).…”

Section: Introductionmentioning

confidence: 99%

“…This involves monitoring the transfer of energy from an excited fluorescent molecule (donor) to another molecule (acceptor). The efficiency of energy transfer is proportional to the inverse sixth power of the distance between donor and acceptor, making it extremely sensitive to interactions in the 1-10 nm range [5].…”

Section: Introductionmentioning

confidence: 99%

High-precision FLIM–FRET in fixed and living cells reveals heterogeneity in a simple CFP–YFP fusion protein

Millington

Grindlay

Altenbach

et al. 2007

Biophysical Chemistry

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT High-Precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple CFP-YFP fusion proteinMichael Millington, [a, b] G. Joan Grindlay, [c] Kirsten Altenbach, [a, d] Robert K. Neely, [a, b] Walter Kolch, [ c, e] Mojca Bencina , [ f ] Nick D. Read, [a, d] Anita C. Jones, [a, b] David T. F.Dryden, [a, b]

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“…Fluorescence emission from trapped ions also present an opportunity to quantify conformational changes of biopolymer species in the gas phase [13]. The extension of FRET, widely used in solution studies [14] to measure trapped biopolymer ions can provide the capability to directly correlate changes in fluorescence intensity with changes in the average conformation of biopolymer molecules. For example, FRET methods might be applied to further investigate gas phase dissociation dynamics of double strand oligonucleotide anions [15].…”

mentioning

confidence: 99%

Pulsed fluorescence measurements of trapped molecular ions with zero background detection

Khoury¹,

Rodriguez‐Cruz²,

Parks³

2002

J Am Soc Mass Spectrom

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Sensitive methods have been developed to measure laser-induced fluorescence from trapped ions by reducing the detection of background scattering to zero levels during the laser excitation pulse. The laser beam diameter has been reduced to ϳ150 m to eliminate scattering on trap apertures and the resulting laser-ion interaction is limited to a volume of ϳ10 Ϫ5 cm 3 which is ϳ0.03-0.15 of the total ion cloud volume depending on experimental conditions. The detection optics collected fluorescence only from within the solid angle defined by laser-ion interaction volume. Rhodamine 640 and Alexa Fluor 350 ions, commonly used as fluorescence resonance energy transfer (FRET) fluorophores, were generated in the gas phase by using electrospray ionization and injected into a radiofrequency Paul trap where they were stored and exposed to Nd:YAG laser pulses at 532 and 355 nm for times up to 10 m. Fluorescence emitted by these ions was investigated for several trap q z values and ion cloud temperatures. Analysis of photon statistics indicated an average of ϳ10 photons were incident on the PMT detector per 15 ns pulse for ϳ10 3 trapped ions in the interaction volume. Fluorescence measurements displayed a dependence on trapped ion number which were consistent with calculations of the space charge limited ion density. To investigate the quantitative capability of these fluorescence techniques, the laser-induced fragmentation of trapped Alexa I on traps provide a controlled environment in which fluorescence measurements can be performed on an ensemble of ions over timescales sufficient to consider small ion numbers and slow reaction rates. Laserinduced fluorescence measurements of trapped ions have been used in spectroscopic studies of atomic structure [1][2][3][4], the vibrational spectra of small molecules [5][6][7][8], as well as to characterize the dynamic cooling [9] and crystallization of atomic ions [10 -12].This paper introduces techniques that eliminate the detection of laser background scattering on trap apertures and internal surfaces allowing measurements to achieve both high sensitivity and large dynamic range. In previous pulsed laser measurements of molecular fluorescence collected from trapped species (discussed in references [5][6][7][8]), the radiative lifetimes were sufficiently long to allow collection of fluorescence radiation after the laser pulse. This greatly simplified methods to reduce the detection of background resulting from scattered laser radiation. However, experiments described in this paper apply the Nd:YAG pulsed laser to excite strongly emitting molecules having fluorescence lifetimes shorter than the laser pulse. This required the reduction of background laser scattering during laser excitation, a more demanding constraint.Techniques described in this paper will help to extend fluorescence measurements as an in situ probe of trapped ion properties including chemical identity, the growth and decay of specific ion populations, ion spectroscopy. Fluorescence emission from trapped ions also presen...

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[13] Fluorescence resonance energy transfer

Cited by 569 publications

References 69 publications

Enhanced Lanthanide Luminescence Using Silver Nanostructures: Opportunities for a New Class of Probes with Exceptional Spectral Characteristics

Enhanced Lanthanide Luminescence Using Silver Nanostructures: Opportunities for a New Class of Probes with Exceptional Spectral Characteristics

High-precision FLIM–FRET in fixed and living cells reveals heterogeneity in a simple CFP–YFP fusion protein

Pulsed fluorescence measurements of trapped molecular ions with zero background detection

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