12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

Tortorici, Lina; Gerlando, Rosalia Di; Tolone, Marco; Mastrangelo, Salvatore; Sardina, Maria Teresa

doi:10.1016/j.livsci.2016.09.015

Cited by 10 publications

(3 citation statements)

References 28 publications

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“…Bottero et al (2003) developed a triplex PCR for identification of cow, goat and sheep milk in cheese samples, the sensitivity of the method was 0.5% of bovine in caprine milk. Tortorici et al (2016) eveloped a duplex PCR for the amplification of cow, ewe and goat DNA sequences specific to 12SrRNA gene. Substitution of buffalo with bovine milk in Mozzarella cheese was tested by species identification of bovine specific mitochondrial cytochrome b gene and mitochondrial cytochrome oxidase subunit I gene.…”

Section: Milk Authenticationmentioning

confidence: 99%

Role of genome technology in dairy food quality and safety

De¹

2019

IJDS

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Genomics tools, specifically the different affordable Sequencing methods, are rapidly transforming almost every aspect of Biological Sciences including food science. Applications of genomic technologies in food sciences is generally considered a great leap forward with some concerns regarding the need of well-equipped and trained laboratories to discriminate between good and bad microorganisms in the fermented food stuff. Species identification of raw and finished dairy and animal products has become an important issue regarding the assessment of food composition. Protection against species substitution or admixture in dairy and animal products is of significant importance for reasons relating to consumer preference, public health and government regulations. Milk and milk products are of particular interest, because they are a group of foods that play an important role in feeding the population and are essential for certain groups of consumers (women, children, and the elderly). Milk products are often prone to adulteration practices, since milk is a fairly expensive raw material and can be easily replaced in part by other dairy or non-dairy ingredients. DNA based methods became very popular, since they are considered more reliable as a result of DNA stability under high temperatures, pressures and chemical treatments used during processing of food products. The polymerase chain reaction (PCR) is the most widely used molecular technique, because of its simplicity, sensitivity and reproducibility. Ruminant milk can be easily used as a source of DNA, since it has a large amount of somatic cells, mostly leucocytes but also epithelial cells from the milking mother, which contain genomic DNA suitable for any kind of genomic DNA testing. PCR amplification of various regions of mitochondrial genome, 12S rRNA, growth hormone (GH) gene and PCR RFLP have been reported and proved the sensitivity of DNA based methods and their reliability for species identification in a wide range of raw and finished animal food products. Complete genome sequencing of bacterial isolates (pathogenic or lactic acid bacteria) has started to replace contemporary popular molecular methods, like ribotyping, MLST and Pulse Field Gel Electrophoresis (PFGE), as subtyping method of choice. Whole Genome Sequencing can able to route of transmission in case of increasingly smaller outbreaks.

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Section: Milk Authenticationmentioning

confidence: 99%

Role of genome technology in dairy food quality and safety

De¹

2019

IJDS

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“…As a result, easy, cheap, accurate, and simultaneous identification of the target species can be achieved with a good sensitivity threshold (0.5%) in milk and cheeses instead of IEF. Recently, Tortorici et al (2016) described a sensitive speciesspecific duplex-PCR method to detect cow, ewe, and goat dairy products simultaneously, amplifying 12S and 16S rRNA genes with 0.1% threshold. The speciesspecific primers were tested in Italian breeds for both studies, so possible nucleotide polymorphisms between the different goat breeds should be taken into account, as they could affect PCR amplification success.…”

Section: New Approaches To Dairy Products Traceability-pcr-based Species Detectionmentioning

confidence: 99%

Caprine and ovine Greek dairy products: The official German method generates false-positive results due to κ-casein gene polymorphism

Tsartsianidou

Triantafillidou

Tarantili

et al. 2017

Journal of Dairy Science

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Caseins are widely used for species identification of dairy products. Isoelectric focusing (IEF) of para-κ-casein peptide is used as the official German method for the differentiation between caprine (isoform A) and ovine (isoform B) dairy products, based on their different isoelectric points. The discrimination between Greek goat and ewe dairy products using IEF has, however, been shown to be problematic because of the existence of the ewe isoform in milk from Greek indigenous dairy goats. This could be due to nucleotide polymorphisms within the goat κ-casein gene of Greek indigenous breeds, which alter the isoelectric point of the para-κ-casein peptide and lead to false positive results. Previous DNA analysis of the goat κ-casein gene has shown high levels of polymorphism; however, no such information is available for Greek indigenous dairy goats. Therefore, 87 indigenous dairy goats were sequenced at exon IV of κ-casein gene. In total, 9 polymorphic sites were detected. Three nonsynonymous point mutations were identified, which change the isoelectric point of the goat para-κ-casein peptide so that it appears identical to that of the ewe peptide. Ten composite genotypes were reconstructed and 6 of them included the problematic point mutations. For the verification of genetic results, IEF was carried out. Both goat and ewe patterns appeared in the problematic genotypes. The frequency of these genotypes could be characterized as moderate (0.23) to high (0.60) within Greek indigenous breeds. However, this is not an issue restricted to Greece, as such genotypes have been detected in various non-Greek goat breeds. In conclusion, IEF based on the official German method is certainly inappropriate for ovine and caprine discrimination concerning Greek dairy goat products, and consequently a new method should be established.

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“…Mitochondrial DNA (mtDNA) polymorphisms have been widely used to determine phylogenetic relationships in cattle breeds. The results of this 12S rRNA sequence have been used to identify the type of milk in the cattle of sheep and goats (Tortorici et al, 2016), to identify the types of livestock meat , to identify the presence of a mixture of dog and rat meat in beef (Cahyadi et al, 2019), and to detect pork and beef in food products (Roy et al, 2021). Based on these reasons, the 12S rRNA gene can be used as a marker of genetic diversity, and until now, there has been no report on Bali cattle in particular.…”

Section: Introductionmentioning

confidence: 99%

Haplotype and phylogenetic diversity using mitochondrial 12S rRNA gene marker in Bali cattle

Suselowati,

Noor,

Jakaria

2023

J. Indonesian Trop. Anim. Agric.

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Bali cattle is one of Indonesia's original livestock genetic resources. The 12S rRNA can be used as a marker of genetic diversity, and until now, there has been no report on Bali cattle. The study was aimed to analyze the gene of mtDNA 12S rRNA to determine haplotype diversity and phylogenetics in Bali cattle populations of the eastern region of Indonesia. A total of 95 blood samples used consisted of three different populations which were Bali cattle, Ongole crossbred (PO) and Madura cattle as a comparison. This research was analyzed using PCR and sequencing methods. The data were analyzed using the cluster W method for estimating genetic distances, calculating diversity, and reconstructing phylogenetic trees using the MEGAX, DNAsP and Network. The genetic distance values ranged from 0.00200 to 0.01508, and the Haplotype diversity values ranged from 0.66000 to 0.91111. The nucleo-tide diversity values ranged from 0.00174 to 0.01673. There were 16 haplotypes found. The values of Gst, Nst and Fst were 0.00803, 0.07550, 0.07622; respectively. Based on the analysis, there were dif-ferences between Bali cattle from various populations, there were specific haplotypes. The 12S rRNA gene can be used as a genetic marker for diversity studies in Bali cattle and other cattle breeds although the diversity is low.

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12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

Cited by 10 publications

References 28 publications

Role of genome technology in dairy food quality and safety

Role of genome technology in dairy food quality and safety

Caprine and ovine Greek dairy products: The official German method generates false-positive results due to κ-casein gene polymorphism

Haplotype and phylogenetic diversity using mitochondrial 12S rRNA gene marker in Bali cattle

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