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Abstract

ABSTRAKLegionella pneumophila adalah bakteri Gram-negatif berbentuk batang yang dapat menyebabkan penyakit nosokomial dan pneumonia. Tujuan penelitian ini adalah untuk mendeteksi keberadaan bakteri L. pneumophila pada air kolam renang di Kota Surabaya dengan menggunakan nested Polymerase Chain Reaction (PCR) berbasis gen spesifik L. pneumophila (mip gene). Penelitian ini menggunakan metode purposive sampling. Sebanyak sepuluh sampel diambil dari lima kolam renang. Sampel diambil sebanyak 200 mL dari air kolam renang di setiap lokasi. Hasil dari 10 sampel yang diuji menggunakan nested PCR, satu sampel menunjukkan hasil positif untuk L.pneumophila, dan sembilan sampel menunjukkan hasil negatif. Bakteri L. pneumophila ditemukan pada sampel air kolam dengan suhu yang lebih tinggi (>30ºC). Satu sampel positip tersebut ketika dilanjutkan terhadap analisis serogrup terlihat bahwa bakteri L. pneumophila yang terdeteksi pada air kolam renang di Kota Surabaya termasuk L. pneumophila serogrup 9 (98%) dan serogrup 10 (98%). Terdeteksinya bakteri L. pneumophila ini diharapkan dapat meningkatkan kesadaran dokter dan ahli mikrobiologi tentang penyebaran L. pneumophila dan juga bermanfaat untuk mengontrol agen Legionellosis. ABSTRACTLegionella pneumophila is a Gram-negative bacillus that causes nosocomial and community-acquired pneumonia. The aim of this research was to detect the presence of bacteria of L. pneumophila species in the swimming pools water of Surabaya city by using nested Polymerase Chain Reaction (PCR) assay of a specific gene for L. pneumophila (mip gene). This study used purposive sampling method. A total of 10 water samples were collected from five swimming pools consisting of 200 mL water for each swimming pool. The results showed that of 10 samples tested by nested PCR, one sample was positive for L. pneumophila, and nine samples were negative. L. pneumophila were found in pool water samples with a higher temperature (>30ºC).Serogrouping analysis of positive sample that L. pneumophila bacteria detected in the water sample of swimming pool in Surabaya was L. pneumophila serogroup 9 (98%) and serogroup 10 (98%). L. pneumophila detection of bacteria is expected to raise the awareness of physician and microbiologists about the transmission of L. pneumophila and will also be useful for controlling the agents.

Screening of Legionella pneumophila from Well Water in Magetan Regency, Indonesia

“…In aquatic habitats the amoeba itself acts as a host. 22 Thus, by the time Legionella bacteria leave the intracellular environment, they experience stress caused by changes in diet, pH, temperature, salinity, and oxygen. To adapt to the changing environment, Legionella enters a living state but cannot be cultured, meaning the bacteria are alive but not evolving.…”

“…The analysis method uses the principle of thermal-based polymerization to Open access under CC-BY-NC-SA 4.0 International License multiply DNA from bacteria contained in water samples so that it can be detected with gel documentation, first and second step PCR using GoTaq® DNA polymerase by Promega, USA, (contains taq DNA polymerase, dNTPs, MgCl2), Legionella primers (forward & reverse) 22 , nuclease free water, and RNA from samples. PCR was run with a pre-denaturation initial temperature of 95°C for 5 minutes and denaturation of 1 minute, annealing 55°C for 1 minute, extension of 72°C for 1 minute, final extension of 72°C for 10 minutes.…”