1992
DOI: 10.1007/bf00318792
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12-O-tetradecanoylphorbol-13-acetate induces selective desquamation of superficial cells in rat urinary bladder epithelium

Abstract: 12-O-tetradecanoylphorbol-13-acetate (TPA) is known to affect the proliferation and/or differentiation of several types of cells. We injected TPA directly into the lumen of rat bladder to determine, using scanning and transmission electron microscopy, its effects on the bladder epithelium in vivo. At 1 h after TPA injection (1 microgram/ml), the superficial cells of the epithelium had changed their morphology, and large spherical vacuoles occupied their cytoplasm. In some areas, the underlying intermediate cel… Show more

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Cited by 5 publications
(4 citation statements)
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“…Several in vivo and in vitro models have thus been applied to study the differentiation process of urothelial cells. In in vivo studies, mainly cyclophosphamide Veranic et al2004), 12-o-tetradecanoylphorbol-13-acetate (TPA) (Watanabe and Sasaki 1992), sodium saccharin (Romih et al 1998) or hyperthermic shock (Jacob et al 1982) have been used to remove urothelial cells from the bladder and the renewal process has then been sequentially studied. Most procedures that efficiently induce cell removal resulted in an inflammatory response, prolonged cell desquamation and caused hyperplasia of the urothelium, all of which disturbs the process of normal differentiation.…”
Section: Introductionmentioning
confidence: 99%
“…Several in vivo and in vitro models have thus been applied to study the differentiation process of urothelial cells. In in vivo studies, mainly cyclophosphamide Veranic et al2004), 12-o-tetradecanoylphorbol-13-acetate (TPA) (Watanabe and Sasaki 1992), sodium saccharin (Romih et al 1998) or hyperthermic shock (Jacob et al 1982) have been used to remove urothelial cells from the bladder and the renewal process has then been sequentially studied. Most procedures that efficiently induce cell removal resulted in an inflammatory response, prolonged cell desquamation and caused hyperplasia of the urothelium, all of which disturbs the process of normal differentiation.…”
Section: Introductionmentioning
confidence: 99%
“…However, after injury, the proliferation and differentiation of basal cells increase rapidly, leading to the fast renewal of intermediate and superficial cells and the reestablishment of the permeability barrier function [3,12]. Several experimental methods for inducing urothelial injury have been developed as means to compromise and study the barrier function [16][17][18][19][20][21]. The intravesical application of the biopolymer chitosan serves as an easily controlled method to detach the superficial layer of urothelial cells [22].…”
mentioning
confidence: 99%
“…To improve the penetration of drugs into urothelial tissue, several inducers of urothelial cell desquamation could be applied. Among them, mainly cyclophosphamide [12,17], 12-o-tetradecanoylphorbol-13-acetate (TPA) [18], sodium saccharin [19], or hyperthermic shock [20] have been used to largely remove urothelial cells. Most procedures that induced cell removal resulted in an inflammatory response, prolonged cell desquamation, and transitional hyperplasia of the urothelium.…”
Section: Urinary Bladder Function and Malfunctionsmentioning
confidence: 99%