12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region.

Abstract

Genomic clones coding for human fibroblast collagenase were isolated. By constructing and transfecting mutants with 5' and 3' deletion mutations of the 5' control region of the gene into human or murine cells, we delimited a 32-base-pair sequence at positions -73 to -42 which is required for the induction of transcription by the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. The DNA element behaves as a 12-0-tetradecanoyl-phorbol-13-acetate-inducible enhancer: it mediates the stimulation of transcription to the heterologous herpes simplex virus thymidine kinase promoter and acts in a position-and orientationindependent manner. Differences in enhancer efficiency in different cell lines are interpreted to indicate differences in the activity of a trans-acting factor.

Cooperative activity between HER oncogenes and the tumor suppressor IRF-1 results in apoptosis

“…The ®rst reporter construct which was used for these experiments contains the promoter of the cis gene which is sensitive to STAT activity (SRE; Figure 6a). The second construct contains a part of the collagenase promoter (773/ +63) (Angel et al, 1987;Schneikert et al, 1996) which is sensitive to the ras pathway mediated by API (ras as responsive element in Figure 6b). As depicted in Figure 6a and 7b both constructs were induced by HER1 signaling up to threefold but the induction was not altered after activation of IRF-1.…”

“…An expression construct coding for the dominant negative ras, rasAsn17 (de Vries-Smits et al, 1992), together with a ras-sensitive promoter (7517/+63 from the collagenase promoter; Figure 7a) (Angel et al, 1987;Schneikert et al, 1996) construct or with the GAScontaining construct (Figure 7b) was transiently transfected into the NIH3T3 cell line expressing Cooperation of HER and IRF-1 S Kirchhoff and H Hauser HER1 and IRF-1-hER. EGF treatment led to a 25-fold induction of the ras sensitive promoter.…”

Modulation of Fos-mediated AP-1 transcription by the promyelocytic leukemia protein

“…The human collagenase promoter is the ®rst and bestcharacterized promoter whose transcription is activated by Fos and Jun (Angel et al, 1987). Therefore, the e ects of PML were examined on a collagenase-CAT construct (residues 773 to +63 of the human Figure 1c demonstrate that cotransfection of the collagenase-CAT reporter with PML signi®cantly increased the promoter activity (fourfold).…”

Hepatitis B virus surface antigen as a reporter of promoter activity

“…An AP-1 binding consensus se- The CAT assays were performed as described by Gorman et al (1982). Two days posttransfection the cells were centrifuged, washed quence (Angel et al, 1987) is included, located 80 bp upstream from the TATA box (Corden et al, 1980). Furthermore, a CAAT motif (Efstratiadis et al, 1980) was noticed 50 bp from the TATA box.…”