[12] Determination of total protein

Peterson, Gary L.

doi:10.1016/s0076-6879(83)91014-5

Cited by 1,152 publications

(439 citation statements)

References 20 publications

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“…In parallel, identical samples were treated with 0.2 ml of 10% of TCA before the incubations. Amino groups in the TCA supernatant were determined using ophthalaldehyde (14). Fluorescence was measured using a SFM 25 Kontron spectrofluorimeter.…”

Section: Preparation Of Erythrocyte Cell-free Extractmentioning

confidence: 99%

Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR)

Kamath

Rao

Kamath

et al. 2006

Indian J Clin Biochem

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The present study demonstrates the possibility of increased lipid peroxidation and protein oxidation in both maternal and fetal erythrocytes as markers of oxygen radical activity during intrauterine growth retardation. The erythrocyte MDA levels were significantly elevated in mothers of IUGR babies when compared to controls (p<0.01). The endogenous protein damage due to oxidative stress was significantly higher in IUGR mothers when compared to controls (p<0.05). Similarly the proteolytic activity in erythrocyte lysates against oxidatively damaged hemoglobin was significantly increased in mothers of IUGR babies compared to controls (p<0.001).In fetuses born with IUGR, both lipid peroxidation and proteolytic activity were significantly increased when compared to normal newborns (p<0.01).The result of this study indicates that oxidative stress was induced both in IUGR babies and their mothers which is manifested as increased lipid peroxidation and protein oxidant damage.

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Section: Preparation Of Erythrocyte Cell-free Extractmentioning

confidence: 99%

Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR)

Kamath

Rao

Kamath

et al. 2006

Indian J Clin Biochem

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“…Total protein was determined by the method of Lowry et al 48,49 Determination of primary amino groups was done by the TNBS method. 47 Conductivity measurements were Gene Therapy done in a V-TECH Oyster pH, temperature, conductivity meter (St Louis, MO, USA) at 37°C.…”

Section: Characterization Of Mediamentioning

confidence: 99%

Lipoplex-induced hemagglutination: potential involvement in intravenous gene delivery

Eliyahu¹,

Servel²,

Domb

et al. 2002

Gene Ther

140

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“…The concentration of total protein in samples of tissue homogenates was determined by the method of Lowry, as modified by Peterson,17 with crystalline bovine serum albumin as the standard.…”

Section: Biochemical Analysismentioning

confidence: 99%

Connective tissue proteinases and inhibitors in abdominal aortic aneurysms. Involvement of the vasa vasorum in the pathogenesis of aortic aneurysms.

Herron

Unemori

Wong

et al. 1991

Arterioscler Thromb

142

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T he development of irreversible dilations of the infrarenal aorta has been attributed to hemodynamic disturbances as well as to structural and metabolic changes within the vessel wall. 1 -2 Chemical and histological analyses have demonstrated significant decreases in the two major structural proteins, collagen and elastin, in dilated vascular tissue from patients undergoing corrective surgery for abdominal aortic aneurysm (AAA) disease. 34 Experimentally, it has been shown that circumferential arterial wall stress is borne primarily by elastin and that collagen maintains the integrity of the arterial wall, as enzymatic hydrolysis of collagen results in vessel rupture, whereas degradation of

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[12] Determination of total protein

Cited by 1,152 publications

References 20 publications

Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR)

Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR)

Lipoplex-induced hemagglutination: potential involvement in intravenous gene delivery

Connective tissue proteinases and inhibitors in abdominal aortic aneurysms. Involvement of the vasa vasorum in the pathogenesis of aortic aneurysms.

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