117 Evaluation of the Effects of Dried Live Yeast on Rumen pH and in Situ Digestibility of Dry Matter in growing cattle.

Cagle, Caitlyn M; Tedeschi, L. O.; Runyan, Chase; Callaway, Todd R.; Cravey, M D

doi:10.1093/jas/sky027.117

Cited by 2 publications

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“…Oppositely, the decrease of ruminal pH was characterized by an accumulation of lactate concentration in the report of Luo et al [ 13 ]. Fortunately, in addition to benefits to production, S. cerevisiae was also proved to stabilize rumen pH and dry matter (DM) digestibility by Cagle et al [ 14 ], indicating a better microbial fermentation. But researches focusing on continuous monitoring and comparison between rumen volatile fatty acids (VFA) and lactic acid metabolism in the presence of live yeast, and alteration of lactate producing and consuming bacteria ( Streptococcus bovis , Lactobacillus spp.…”

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confidence: 99%

Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattle

et al. 2022

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Objective: In this study we aimed to evaluate the effect of dietary live yeast supplementation on ruminal pH pattern, fermentation characteristics and associated bacteria in beef cattle.Methods: This work comprised of in vitro and in vivo experiments. In vitro fermentation was conducted by incubating 0, 0.05, 0.075, 0.1, 0.125, and 0.15% active dried yeast (Saccharomyces cerevisiae, ADY) with total mixed ration substrate to determine its dose effect. According to in vitro results, 0.1% ADY inclusion level was assigned in in vivo study for continuously monitoring ruminal fermentation characteristics and microbes. Six ruminally cannulated steers were randomly assigned to 2 treatments (Control and ADY supplementation) as two-period crossover design (30-day). Blood samples were harvested before-feeding and rumen fluid was sampled at 0, 3, 6, 9 and 12 h post-feeding on 30 d. Results:After 24 h in vitro fermentation, pH and gas production were increased at 0.1% ADY where ammonia nitrogen and microbial crude protein also displayed lowest and peak values, respectively. Acetate, butyrate and total volatile fatty acids concentrations heightened with increasing ADY doses and plateaued at high levels, while acetate to propionate ratio was decreased accordingly. In in vivo study, ruminal pH was increased with ADY supplementation that also elevated acetate and propionate. Conversely, ADY reduced lactate level by dampening Streptococcus bovis and inducing greater Selenomonas ruminantium and A c c e p t e d A r t i c l eMegasphaera elsdenii populations involved in lactate utilization. The serum urea nitrogen decreased, whereas glucose, albumin and total protein concentrations were increased with ADY supplementation. Conclusion:The results demonstrated dietary ADY improved ruminal fermentation dose-dependently. The ruminal lactate reduction through modification of lactate metabolic bacteria could be important reason for rumen pH stabilization induced by ADY. ADY supplementation offered a complementary probiotics strategy in improving gluconeogenesis and nitrogen metabolism of beef cattle, potentially resulted from optimized rumen pH and fermentation.

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Section: Introductionmentioning

confidence: 99%

Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattle

et al. 2022

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Evaluation of different inclusion levels of dry live yeast impacts on various rumen parameters and in situ digestibilities of dry matter and neutral detergent fiber in growing and finishing beef cattle

Cagle

Batista

Anderson

et al. 2019

Journal of Animal Science

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This study evaluated the effects of supplementing dry live yeast (LY; Saccharomyces cerevisiae) on in vitro gas production (IVGP) fermentation dynamics, pH, and CH4 concentration at 48 h, and in situ rumen parameters and digestibility of DM (DMD) and NDF (NDFD) of growing cattle during 3 feeding phases: grower (GRW) for 17 d (38% steamed-flaked corn; SFC), transition (TRANS) for 15 d (55.5% SFC: 1.2 Mcal/kg NEg), and finisher (FIN) for 13 d (73% SFC: 1.23 Mcal/kg NEg). Twenty British-crossbred, ruminally cannulated steers (183 kg ± 44 kg) 6 mo of age were blocked by weight into 5 pens containing Calan gate feeders and received a control (CON) diet (17.2% CP, 35.8% NDF, 86.7% DM) without LY on days −12 to 0. After that, animals were randomly assigned to treatments (TRT), 5 animals per TRT: CON or LY at inclusion rates of 5 g/d (LY1), 10 g/d (LY2), or 15 g/d (LY3) top dressed every morning at 0800 for 45 d. The DMD and NDFD were assessed during 7 separate collection days using in situ nylon bags containing 5 g of GRW, TRANS, or FIN diets, incubated at 1200 for 48 h. Protozoa counts (PC) were determined during 5 collection periods. Data were analyzed as a repeated measure within a randomized complete block design, assuming a random effect of the pen. For GRW, TRT altered the total gas production of the nonfiber carbohydrate (NFC; P = 0.045) and the fractional rate of degradation (kd) of the fiber carbohydrate (FC) pool (P = 0.001) in a cubic pattern (P ≤ 0.05): LY2 had the most gas production and fastest kd. TRT also influenced DMD (P = 0.035) and NDFD (P = 0.012) with LY2 providing the greatest digestibility. For TRANS, TRT tended to affect the NFC kd (P = 0.078) and influenced pH (P = 0.04) and DMD (P < 0.001) in which LY2 yielded the fastest kd, highest pH, and greatest DMD. For FIN, there was an effect of TRT on total gas production (P < 0.001) and kd (P = 0.004) of the NFC pool, FC kd (P = 0.012), in vitro CH4 concentration (P < 0.001), PC (P < 0.001), DMD (P = 0.039), and NDFD (P = 0.008). LY1 had the highest PC and provided the greatest DMD and NDFD. LY2 had the fastest kd of both the NFC and FC pools and had the least CH4 concentration. LY3 had the greatest NFC gas production. No specific dose–response pattern was observed, but 10 g/d provided the most beneficial result for all diets. We concluded that supplementation with LY affected IVGP as well as ruminal parameters and digestibilities.

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117 Evaluation of the Effects of Dried Live Yeast on Rumen pH and in Situ Digestibility of Dry Matter in growing cattle.

Cited by 2 publications

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Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattle

Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattle

Evaluation of different inclusion levels of dry live yeast impacts on various rumen parameters and in situ digestibilities of dry matter and neutral detergent fiber in growing and finishing beef cattle

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