[116] 6-phosphogluconic dehydrase

Meloche, H. Paul; Wood, William F.

doi:10.1016/0076-6879(66)09132-8

Cited by 17 publications

(7 citation statements)

References 6 publications

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“…The enzyme is inhibited by fluoride ions. Meloche and Wood [7] provided evidence that the mechanism of action, of EDD involves an enoI-KDPG intermediate that undergoes spontaneous rearrangement to the keto form by a reaction sequence that is essentially irreversible. Purification of the EDD from Zymomonas mobilis indicated that the enzyme functions as a dimer of identical 63-kDa subunits [8].…”

Section: 36-phosphogluconate Dehydratasementioning

confidence: 99%

The Entner-Doudoroff pathway: history, physiology and molecular biology

Conway¹

1992

FEMS Microbiology Letters

321

194

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The Entner‐Doudoroff pathway is now known to be very widely distributed in nature. Biochemical and physiological studies show that the Entner‐Doudoroff pathway can operate in a linear and catabolic mode, in a ‘cyclic’ mode, in a modified mode involving non‐phosphorylated intermediates, or in alternative modes involving C1 metabolism and anabolism. Molecular and genetic analyses of the Entner‐Doudoroff pathway in Zymomonas mobilis, Escherichia coli and Pseudomonas aeruginosa have led to an improved understanding of some fundamental aspects of metabolic controls. It can be argued that the Entner‐Doudoroff pathway is more primitive than Embden‐Meyerhof‐Parnas glycolysis.

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Section: 36-phosphogluconate Dehydratasementioning

confidence: 99%

The Entner-Doudoroff pathway: history, physiology and molecular biology

Conway¹

1992

FEMS Microbiology Letters

321

194

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“…6-Phosphogluconate dehydratase and 2-keto-3-deoxy-6phosphogluconate (KDPG) aldolase, the two enzymes comprising the pathway (see Figure 1), have been characterized extensively in P. putida by W. A. Wood and his coworkers (64,65,83,84). Kersters & De Ley have examined the distribution of these enzymes in a variety of gram-negative bacteria (61,62).…”

Section: Central Role Of the Entner-doudoroff Pathwaymentioning

confidence: 99%

Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Lessie¹,

Phibbs²

1984

Annu. Rev. Microbiol.

309

142

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“…Enzyme activities were measured at room temperature in a Gilford 2400 recording spectrophotometer. Published procedures for glucose dehydrogenase (6), glucose-6-phosphate dehydrogenase (8), glucokinase (1), and gluconate-6-phosphate dehydrase (12) were used with minor modifications to provide optimal conditions of pH and substrate concentration. Gluconate dehydrogenase has been assayed by the same method as glucose dehydrogenase but with gluconate as substrate.…”

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confidence: 99%

“…2-Keto-3-deoxy-gluconate-6-phosphate (KDGP) aldolase activity was measured as described by Meloche et al (11). The substrate for the reaction, KDGP, was produced from gluconate-6-phosphate by means of a KDGP aldolase-free preparation of gluconate-6-phosphate dehydrase, purified from extracts of strain 412-6 (Table 1) by following the procedure of Meloche and Wood (12). The reaction mixture to produce KDGP contained in a final volume of 10 ml: 500 umol of Tris-hydrochloride buffer, pH 8.0; 100 ;&mol of reduced glutathione; 5 Mumol of MnCls; 100 gmol of gluconate-6-phosphate; and 4 international units (IU) of the dehydrase.…”

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confidence: 99%

Glucolysis in Pseudomonas putida : Physiological Role of Alternative Routes from the Analysis of Defective Mutants

Vicente¹,

Cánovas²

1973

J Bacteriol

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A number of mutants in which glucolysis is impaired have been isolated from Pseudomonas putida. The study of their behavior shows that this organism possesses a single glucolytic pathway with physiological significance. The first step of the pathway consists in the oxidation of glucose into gluconate. Two proteins with glucose dehydrogenase activity appear to exist in P. putida but the reasons for this duplicity are not clear. The process continues with the formation of 2-ketogluconate which is in turn converted into gluconate-6-phosphate. This is proved by the fact that mutants unable to form gluconate-6-phosphate from 2-ketogluconate show extremely slow growth on glucose or gluconate (generation times are increased more than 100 times). Other possible routes for the conversion of glucose into gluconate-6-phosphate, the glucose-6-phosphate pathway, or the direct phosphorylation of the gluconate formed by glucose oxidation are only minor shunts in P. putida. The Entner-Doudoroff enzymes, which catalyze the conversion of gluconate-6-phosphate into pyruvate and triosephosphate, appear to be essential to grow on glucose and also on gluconate and 2-ketogluconate. A significative role of the pentose route in the catabolism of these substrates is not apparent from this study. In contrast, P. putida strains showing no activity of the Entner-Doudoroff enzymes grow readily on fructose, although there is evidence that this hexose is at least partially catabolized via gluconate-6-phosphate.The present picture of hexose catabolism in the pseudomonads is far from simple, not only because different systems catalyze the same process from one species to another but also because one species may have alternative routes with apparently identical function. The Entner-Doudoroff pathway (3) appears to be the predominant route to metabolize most common hexoses in Pseudomonas (Fig. 1; for review, see ref. 16). However, the hexose monophosphate shunt probably accounts in P. aeruginosa for 30% of the glucolytic function (23, 26), the remaining 70% being carried out by means of the Entner-Doudoroff enzymes. Gluconate dissimilation is totally achieved through the Entner-Doudoroff pathway in several Pseudomonas species (26).Gluconate-6-phosphate, first intermediate of glucose metabolism common to both the Entner-Doudoroff and the pentose pathways, is formed by alternative routes (Fig.

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[116] 6-phosphogluconic dehydrase

Cited by 17 publications

References 6 publications

The Entner-Doudoroff pathway: history, physiology and molecular biology

The Entner-Doudoroff pathway: history, physiology and molecular biology

Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Glucolysis in Pseudomonas putida : Physiological Role of Alternative Routes from the Analysis of Defective Mutants

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