111In (III) uptake by inflammatory and normal tissues

Abstract

Tissue distributions of 111In (III) in the rats bearing granuloma, inflammatory tissue induced by turpentine oil, were compared with those of 67Ga. The results showed that indium-111 resembles 67Ga in the manner of uptake by inflammatory and normal soft tissues. The effect of cold-InCl3 on 111In (III) uptake showed that transferrin is not involved in the uptake of 111In (III) into inflammatory tissues but is involved in the uptake into liver and spleen.

Transferrin Is Not Involved in the Entry of 67Ga into Hepatocytes from Regenerating Liver of Partially Hepatectomized Rats.

“…Acid glycosaminoglycans (AGAGs) were extracted according to the method described by Ohkubo et al 16) with a slight modification. The liver tissue was extracted with 0.5 mol/l NaOH at 4°C for 4 h. The extracted solution was dialyzed against running tapwater overnight followed by distilled water for an additional 24 h, and the lyophilized materials were then digested with pronase-E (Kaken Kagaku Co., Tokyo, Japan)/phosphate buffer, pH 7.4, at 37°C for 24 h followed by an additional 24 h at 37°C.…”

“…Ohkubo et al reported that Tf is not involved in the uptake of 67 Ga by rat granuloma tissue, that is, inflammatory tissue produced by turpentine oil, 11) but is involved in the liver of normal mice. 12) Hammersley et al reported the uptake of 67 Ga in regenerating rat liver after partial hepatectomy, 13) but the involvement of Tf in the uptake was not shown.…”

A Possible Mechanism for the Exchange of Transferrin-67Ga Complex to Heparan Sulfate-67Ga Complex.

“…We have reported that sulfuric acid residues of acid glycosaminoglycans (AGAGs) were not involved in the binding of 67 Ga to AGAGs. 22) Consequently, it is suggested that 67 Ga does not bind to AGAGs via a negative charge of AGAGs. The neutral sugar, which has no charge, cannot bind to cationic detergent.…”

“…We have reported that 67 Ga was exclusively bound with Tf both in vitro and in vivo, but that a high concentration of sodium citrate strongly inhibited the binding of 67 Ga to Tf and that sodium citrate decreased the uptake of 67 Ga into the blood, liver and spleen, but had no influence on the uptake of 67 Ga into inflammatory tissue. 15) Furthermore, the uptake of 67 Ga into the granuloma tissues occurs in a free, Tf-unbound form, but into the liver and spleen in a transferrin-bound form. 16) Weiner 17) has reported that a phosphate-containing compound such as ATP stimulated the translocation of 67 Ga from Tf to ferritin.…”

Stabilities of 67Ga- and 111In-Labeled Transferrin In Vitro

“…We have also reported that 111 In entered into hepatocytes after partial hepatectomy [7] and that Tf was not involved in the uptake of 111 In into inflammatory tissues but was involved in uptake into liver and spleen [8]. Transferrin and its receptor (TfR) are not only important to iron metabolism but also play role in various biological events.…”