111 Single Equine Embryo Lipid Fingerprinting by Mass Spectrometry

Gonçalves, R. F.; Ferreira, Christina R.; Orlandi, C; Sartori, V. C.; Ferreira, Heder Nunes; Gozzo, Fábio C.; Saraiva, Sérgio A.; Pilau, Eduardo Jorge; Eberlin, Marcos N.

doi:10.1071/rdv23n1ab111

Cited by 4 publications

(5 citation statements)

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“…The use of MALDI‐MS and related mass spectrometric protocols of mammalian oocytes and embryos is being rapidly incorporated in embryological research (Gonçalves et al. ; Ferreira et al. ,b).…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition of Lipids Present in Cat and Dog Oocyte by Matrix‐Assisted Desorption Ionization Mass Spectrometry (MALDI‐ MS)

Apparício

Ferreira

Tata

et al. 2012

Reprod Domestic Animals

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ContentsThe aim of the present study was to investigate the level of information on the chemical structures and relative abundances of lipids present in cat and dog oocytes by matrixassisted laser desorption mass spectrometry (MALDI-MS). The MALDI-MS approach requires a simple analysis workflow (no lipid extraction) and few samples (two or three oocytes per analysis in this work) providing concomitant profiles of both intact phospholipids such as sphingomyelins (SM) and phosphatidylcholines (PC) as well as triacylglycerols (TAG). The lipids were detected in oocytes by MALDI using dihydroxybenzoic acid (DHB) as the matrix. The most abundant lipid present in the MS profiles of bitch and queen oocytes was a PC containing 34 carbons and one unsaturation [PC (34:1)]. Oocytes of these two species are characterized by differences in PC and TAG profiles detected qualitatively as well as by means of principal component analysis (PCA). Cat oocytes were mainly discriminated by more intense C52 and C54 TAG species and a higher number of unsaturations, indicating predominantly linoleic and oleic fatty acyl residues. Comparison of the lipid profile of bitch and queen oocytes with that of bovine oocytes revealed some similarities and also some species specificity: TAG species present in bovine oocytes were also present in bitches and queens; however, a more pronounced contribution of palmitic, stearic and oleic fatty acid residues was noticed in the lipid profile of bovine oocytes. MALDI-MS provides novel information on chemical lipid composition in canine and feline oocytes, offering a suitable tool to concomitantly monitor, in a nearly direct and simple fashion the composition of phospholipids and TAG. This detailed information is highly needed to the development of improved protocols for in vitro culture and cryopreservation of cat and dog oocytes.

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Section: Introductionmentioning

confidence: 99%

“…MALDI-MS also allows single oocytes to be analysed, avoiding the pooling of tens to hundreds gametes for one single measurement. The use of MALDI-MS and related mass spectrometric protocols of mammalian oocytes and embryos is being rapidly incorporated in embryological research (Gonc¸alves et al 2011;Ferreira et al 2012a,b).…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of Lipids Present in Cat and Dog Oocyte by Matrix‐Assisted Desorption Ionization Mass Spectrometry (MALDI‐ MS)

Apparício

Ferreira

Tata

et al. 2012

Reprod Domestic Animals

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“…Of the 20 potentially relevant articles, one was an in vitro experimental study [ 46 ]; three articles were excluded because of the wrong population (cumulus cells [ 47 ] or older fetus [ 48 , 49 ]); and 10 published either no MSI results, only MS data [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ], or only proteomics of the embryos [ 59 ]. One abstract was also excluded because no data extraction could be performed [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

“…Although the topic is highly current, only five publications were found in the systematic literature search that met all our inclusion criteria. Several former studies investigated the role of lipids in oocytes [ 66 , 67 ], early stages of embryo development [ 52 , 56 , 58 , 66 , 68 , 69 , 70 , 71 ], placenta in late pregnancy [ 72 , 73 ], as well as endometrial tissues during the window of implantation [ 74 ], and in endometrial fluid [ 75 ]. However, in these studies, lipid composition was mostly investigated with MS and not with MSI.…”

Section: Discussionmentioning

confidence: 99%

Lipid Changes in the Peri-Implantation Period with Mass Spectrometry Imaging: A Systematic Review

Gitta

Márk

Szentpéteri

et al. 2023

Life

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Mass spectrometry imaging is a sensitive method for detecting molecules in tissues in their native form. Lipids mainly act as energy stores and membrane constituents, but they also play a role in lipid signaling. Previous studies have suggested an important role of lipids in implantation; therefore, our aim was to investigate the lipid changes during this period based on the available literature. The systematic literature search was performed on Ovid MEDLINE, Cochrane Library, Embase, and LILACS. We included studies about lipid changes in the early embryonal stage of healthy mammalian development published as mass spectrometry imaging. The search retrieved 917 articles without duplicates, and five articles were included in the narrative synthesis of the results. Two articles found a different spatial distribution of lipids in the early bovine embryo and receptive uterus. Three articles investigated lipids in mice in the peri-implantation period and found a different spatial distribution of several glycerophospholipids in both embryonic and maternal tissues. Although only five studies from three different research groups were included in this systematic review, it is clear that the spatial distribution of lipids is diverse in different tissues and their distribution varies from day to day. This may be a key factor in successful implantation, but further studies are needed to elucidate the exact mechanism.

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“…(7) Lipid profiles of single oocytes and embryos were evaluated by mass measurement of phospholipid profiles using matrix-assisted laser desorption/ ionization mass spectrometry (MALDI-MS) fingerprinting. (8,9) The characteristics of a single bovine embryo after cryopreservation were also measured by MALDI-MS. (10) A single oocyte and single embryo lipid were also analyzed by a more direct and simpler MS technique of desorption electrospray ionization mass spectrometry (DESI-MS). (11) However, as these mass measurements are based on destructive analysis techniques, the analyzed embryos are not able to progress to transplantation.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Holder-type Piezoresistive Cantilever for Embryo Mass Measurement

Sone¹,

Kawakami²,

Saito³

et al. 2018

Sensors and Materials

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A novel embryo evaluation system and microsensor devices have been developed using microcantilever-based biosensors. A holder-type piezoresistive cantilever was fabricated using a focused ion beam (FIB) to place and fix an embryo on the cantilever, and the monitoring system of the cantilever resonance frequency for measuring the embryo mass by the frequency sweep method was developed. The mass of a single 2-cell-stage mouse embryo was measured using the holder-type cantilever in a culture medium with calf serum. The resonance frequency of the cantilever before and after embryo placement was changed from 95.0 to 91.5 kHz. The resonance frequency was approximately constant during 20 min of monitoring. From the frequency change of −3.5 kHz, the mouse embryo mass was estimated to be about 4.4 ng. Although this value is smaller than the mass calculated from the volume of the mouse embryo and the density of culture medium, it indicates the possibility of measuring a single mouse embryo mass using a holder-type cantilever sensor system.

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111 Single Equine Embryo Lipid Fingerprinting by Mass Spectrometry

Cited by 4 publications

References 0 publications

Chemical Composition of Lipids Present in Cat and Dog Oocyte by Matrix‐Assisted Desorption Ionization Mass Spectrometry (MALDI‐ MS)

Chemical Composition of Lipids Present in Cat and Dog Oocyte by Matrix‐Assisted Desorption Ionization Mass Spectrometry (MALDI‐ MS)

Lipid Changes in the Peri-Implantation Period with Mass Spectrometry Imaging: A Systematic Review

Fabrication of Holder-type Piezoresistive Cantilever for Embryo Mass Measurement

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