1108 Ultra-Deep-Pyrosequencing of Precore Region of Hepatitis B Virus

Homs, María; Buti, María; Jardí, R.; Tabernero, David; Schaper, Melanie; Quer, Josep; Ortega, Israel Parra; Esteban, Rafael; Rodríguez‐Frías, Francisco

doi:10.1016/s0168-8278(11)61110-0

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“…They then performed a an ultradeep sequencing analysis. Only in one of the seven patients examined were both YMDD (codon 204) and pre-core (codons 1, 2 and 28) mutations detected with frequencies above 1 % (Homs et al, 2011).…”

Section: Discussionmentioning

confidence: 91%

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Role of the line probe assay INNO-LiPA HBV DR and ultradeep pyrosequencing in detecting resistance mutations to nucleoside/nucleotide analogues in viral samples isolated from chronic hepatitis B patients

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et al. 2013

Journal of General Virology

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Despite the effectiveness of nucleoside/nucleotide analogues in the treatment of chronic hepatitis B (CHB), their long-term administration is associated with the emergence of resistant hepatitis B virus (HBV) mutants. In this study, mutations resulting in antiviral resistance in HBV DNA samples isolated from 23 CHB patients (nine treatment naïve and 14 treated previously) were studied using a line probe assay (INNO-LiPA HBV DR; Innogenetics) and ultradeep pyrosequencing (UDPS) methods. Whilst the INNO-LiPA HBV DR showed no resistance mutations in HBV DNA samples from treatment-naive patients, mutations mediating lamivudine resistance were detected in three samples by UDPS. Among patients who were treated previously, 19 mutations were detected in eight samples using the INNO-LiPA HBV DR and 29 mutations were detected in 12 samples using UDPS. All mutations detected by the INNO-LiPA HBV DR were also detected by UDPS. There were no mutations that could be detected by INNO-LiPA HBV DR but not by UDPS. A total of ten mutations were detected by UDPS but not by INNO-LiPA HBV DR, and the mean frequency of these mutations was 14.7 %. It was concluded that, although INNO-LiPA HBV DR is a sensitive and practical method commonly used for the detection of resistance mutations in HBV infection, UDPS may significantly increase the detection rate of genotypic resistance in HBV at an early stage.

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Section: Discussionmentioning

confidence: 91%

“…To the best of our knowledge, in the literature only a single study exists that has examined pre-core mutations by UDPS (Homs et al, 2011). In that study, the investigators started with the idea that HBV polymerase initiates RT via the encapsidation signal in the pre-genomic RNA, which is located in the pre-core.…”

Section: Discussionmentioning

confidence: 99%