[11] Induction, production, and concentration of interferon produced by a myeloblast culture

Familletti, Philip C.; Costello, Lauren E.; Rose, Cynthia A.; Pestka, Sidney

doi:10.1016/0076-6879(81)78101-1

Cited by 10 publications

(3 citation statements)

References 1 publication

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“…DISCUSSION KG-1 cells induced with 100 HA units of Sendai virus per ml after a 6-h incubation at 37°C with 100 U of leukocyte interferon per ml yielded optimal levels of interferon. The cells grew read- (10). Casein (10%, vol/vol) may be substituted for serum in the induction medium to facilitate purification (32).…”

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confidence: 99%

Production of high levels of human leukocyte interferon from a continuous human myeloblast cell culture

Familletti¹,

McCandliss²,

Pestka³

1981

Antimicrob Agents Chemother

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A myeloblast cell line has proved to be an excellent source of human leukocyte interferon. These cells, primed with interferon and induced with Sendai virus, produced optimal levels of human leukocyte interferon. The cells grew readily in spinner flasks and in medium containing horse serum. Interferon production over several months yielded an average titer of 2 X 10(5) reference units of interferon per 10(7) cells. The interferon produced by these cells appeared to be predominantly species of human leukocyte interferon. Since these cells seemed to consist of myeloblasts, it is clear that cells other than B-lymphocytes can produce leukocyte interferon.

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confidence: 99%

Production of high levels of human leukocyte interferon from a continuous human myeloblast cell culture

Familletti¹,

McCandliss²,

Pestka³

1981

Antimicrob Agents Chemother

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“…The KG-1 cells, a continuous human myeloblast cell line, were derived from a patient with acute myelogenous leukemia (17). Under appropriate conditions, these cells produce high titers 'of interferon that appears to be predominantly, if not entirely, leukocyte interferon (18). The cells were induced to produce interferon with Sendai virus (18) and were harvested at 5 hr after induction.…”

mentioning

confidence: 99%

“…Under appropriate conditions, these cells produce high titers 'of interferon that appears to be predominantly, if not entirely, leukocyte interferon (18). The cells were induced to produce interferon with Sendai virus (18) and were harvested at 5 hr after induction. RNA from these cells was prepared by the guanidine thiocyanate/guanidine hydrochloride procedure (19,20).…”

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confidence: 99%

Construction and identification of bacterial plasmids containing nucleotide sequence for human leukocyte interferon.

Maeda¹,

McCandliss²,

Gross³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

118

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Bacterial plasmids containing human leukocyte interferon sequences were constructed and identified. Identification was confirmed by correspondence of the nucleotide sequence with out amino acid sequence of human leukocyte interferon. The finding of bacterial recombinants containing distinct leukocyte interferon sequences is consistent with our purification of different leukocyte interferon species. We conclude that what has been designated human leukocyte interferon is, indeed, a class of homologous proteins. Preliminary indications suggest that their diversity appears to be represented by individual genomic equivalents. Each of the individual species exhibits characteristic activities. The structural modulation of these biological activities has immense significance for understanding the natural role of the interferons and for refining and developing their ultimate therapeutic potential.

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The Interferon System in Man: Nature of the Interferon Molecules and Mode of Action

Revel

1984

Antiviral Drugs and Interferon: The Molecular Basis of Their Activity

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[11] Induction, production, and concentration of interferon produced by a myeloblast culture

Cited by 10 publications

References 1 publication

Production of high levels of human leukocyte interferon from a continuous human myeloblast cell culture

Production of high levels of human leukocyte interferon from a continuous human myeloblast cell culture

Construction and identification of bacterial plasmids containing nucleotide sequence for human leukocyte interferon.

The Interferon System in Man: Nature of the Interferon Molecules and Mode of Action

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