10th Royan Institute's International Summer School on “Molecular Biomedicine: From Diagnostics to Therapeutics”

Moradi, Sharif; Torabi, Parisa; Mohebbi, Saeed; Amjadian, Sara; Bosma, Piter J.; Faridbod, Farnoush; Khoddami, Vahid; Hosseini, Morteza; Babashah, Sadegh; Ghotbaddini, Maryam; Rasti, Arezoo; Shekari, Faezeh; Abandansari, Hamid Sadeghi; Kiani, Jafar; Shamsara, Mehdi; Kazemi‐Ashtiani, Mohammad; Gholami, Samira

doi:10.1002/bies.202000042

Cited by 8 publications

(6 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MicroRNAs as the Regulatory Switches during Scar Formation MicroRNAs are small noncoding RNA molecules serving as the posttranscriptional gene expression regulators, thereby modulating virtually all biological processes and developmental pathways [124][125][126][127][128]. The evidence is constantly mounting that microRNAs are capable of regulating different aspects of wound healing, and their dysregulation is linked to aberrant wound repair and scar formation.…”

Section: Noncoding Rnas In Scar Formationmentioning

confidence: 99%

The Emerging Therapeutic Targets for Scar Management: Genetic and Epigenetic Landscapes

Amjadian

Moradi

Mohammadi

2022

Skin Pharmacol Physiol

Self Cite

View full text Add to dashboard Cite

Wound healing is a complex process including hemostasis, inflammation, proliferation, and remodeling during which an orchestrated array of biological and molecular events occurs to promote skin regeneration. Abnormalities in each step of the wound healing process lead to reparative rather than regenerative responses, thereby driving the formation of cutaneous scar. Patients suffering from scars represent serious health problems such as contractures, functional and aesthetic concerns as well as painful, thick, and itchy complications, which generally decrease the quality of life and impose high medical costs. Therefore, therapies reducing cutaneous scarring are necessary to improve patients' rehabilitation. Current approaches to remove scars, including surgical and non-surgical methods are not efficient enough, which is in principle due to our limited knowledge about underlying mechanisms of pathological as well as the physiological wound healing process. Thus, therapeutic interventions focused on basic science including genetic and epigenetic knowledge are recently taken into consideration as promising approaches for scar management, since they have the potential to provide targeted therapies and improve the conventional treatments as well as present opportunities for combination therapy. In this review, we highlight the recent advances in skin regenerative medicine through genetic and epigenetic approaches to achieve novel insights for the development of safe, efficient, and reproducible therapies and discuss promising approaches for scar management.

show abstract

Section: Noncoding Rnas In Scar Formationmentioning

confidence: 99%

The Emerging Therapeutic Targets for Scar Management: Genetic and Epigenetic Landscapes

Amjadian

Moradi

Mohammadi

2022

Skin Pharmacol Physiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…These agents can damage normal cells and tissues and some cancer cells can evade them by developing resistance to these drugs 4,5 . Hence, it is necessary to find novel, safe, and effective methods or medicines for cancer treatment 6 …”

Section: Introductionmentioning

confidence: 99%

“…4,5 Hence, it is necessary to find novel, safe, and effective methods or medicines for cancer treatment. 6 The term secretome refers to a diverse set of factors secreted by various cells and tissues that include cytokines, noncoding RNAs such as microRNAs, and extracellular vesicles. The molecules in the secretome are known to be taken up by other cells and regulate various biological processes in the recipient cells.…”

Section: Introductionmentioning

confidence: 99%

Conditioned media from human pluripotent stem cell‐derived cardiomyocytes inhibit the growth and migration of lung cancer cells

Sadeghi

Masoudi

Torabi

et al. 2023

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

Conditioned media (CM) from various cell types contain significant levels of paracrine factors. Recently, therapeutic properties of CM derived from stem cells have been revealed. Based on the fact that heart cancer is extremely rarely, we hypothesized that the CM obtained from human pluripotent stem cell-derived cardiomyocytes might inhibit cancer cell growth and survival. To this end, lung cancer cell line A549 along with human foreskin fibroblasts (HFF) were treated with serial concentrations of cardiomyocyte CM (CCM) or fibroblast CM (FCM). We found that CCM markedly reduced the viability of

show abstract

“…Global expression of miRNAs has been documented to be deregulated in most (if not all) cancers [ 19 ]. Functionally, a fraction of miRNAs promote tumorigenicity (also known as oncomiRs), while others suppress cancer cell growth and metastasis (also known as tumor-suppressive or anti-cancer miRNAs) [ 9 , 14 , 20 , 21 ]. While antisense oligonucleotides are frequently used to prevent oncomiRs from functioning, delivery of tumor-suppressing miRNAs has emerged as a potent strategy to restrict tumor growth and metastasis [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Pan-cancer analysis of microRNA expression profiles highlights microRNAs enriched in normal body cells as effective suppressors of multiple tumor types: A study based on TCGA database

Moradi

Kamal

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

Background MicroRNAs (miRNAs) are frequently deregulated in various types of cancer. While antisense oligonucleotides are used to block oncomiRs, delivery of tumour-suppressive miRNAs holds great potential as a potent anti-cancer strategy. Here, we aim to determine, and functionally analyse, miRNAs that are lowly expressed in various types of tumour but abundantly expressed in multiple normal tissues. Methods The miRNA sequencing data of 14 cancer types were downloaded from the TCGA dataset. Significant differences in miRNA expression between tumor and normal samples were calculated using limma package (R programming). An adjusted p value < 0.05 was used to compare normal versus tumor miRNA expression profiles. The predicted gene targets were obtained using TargetScan, miRanda, and miRDB and then subjected to gene ontology analysis using Enrichr. Only GO terms with an adjusted p < 0.05 were considered statistically significant. All data from wet-lab experiments (cell viability assays and flow cytometry) were expressed as means ± SEM, and their differences were analyzed using GraphPad Prism software (Student’s t test, p < 0.05). Results By compiling all publicly available miRNA profiling data from The Cancer Genome Atlas (TCGA) Pan-Cancer Project, we reveal a small set of tumour-suppressing miRNAs (which we designate as ’normomiRs’) that are highly expressed in 14 types of normal tissues but poorly expressed in corresponding tumour tissues. Interestingly, muscle-enriched miRNAs (e.g. miR-133a/b and miR-206) and miRNAs from DLK1-DIO3 locus (e.g. miR-381 and miR-411) constitute a large fraction of the normomiRs. Moreover, we define that the CCCGU motif is absent in the oncomiRs’ seed sequences but present in a fraction of tumour-suppressive miRNAs. Finally, the gain of function of candidate normomiRs across several cancer cell types indicates that miR-206 and miR-381 exert the most potent inhibition on multiple cancer types in vitro. Conclusion Our results reveal a pan-cancer set of tumour-suppressing miRNAs and highlight the potential of miRNA-replacement therapies for targeting multiple types of tumour.

show abstract

10th Royan Institute's International Summer School on “Molecular Biomedicine: From Diagnostics to Therapeutics”

Cited by 8 publications

References 10 publications

The Emerging Therapeutic Targets for Scar Management: Genetic and Epigenetic Landscapes

The Emerging Therapeutic Targets for Scar Management: Genetic and Epigenetic Landscapes

Conditioned media from human pluripotent stem cell‐derived cardiomyocytes inhibit the growth and migration of lung cancer cells

Pan-cancer analysis of microRNA expression profiles highlights microRNAs enriched in normal body cells as effective suppressors of multiple tumor types: A study based on TCGA database

Contact Info

Product

Resources

About