105. Enhanced glucocorticoid sensitivity coupled with reduced microglial activation in a genetically predisposed animal model of depression

Sherwin, Eoin; Gormley, S.; McGuinness, B. W.; Harkin, Andrew

doi:10.1016/j.bbi.2014.06.125

Cited by 1 publication

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“…The lack of change after environmental condition could mean that this genetic model of depression is not influenced by environmental changes. Studies of microglia in the WKY rat are not common and have found that microglial expression is attenuated after stress [55]. Notably, the authors did not specify whether female or male animals were used.…”

Section: Discussionmentioning

confidence: 99%

Strain Differences and Effects of Environmental Manipulation on Astrocytes (Glial Fibrillary Acidic Protein), Glucocorticoid Receptor, and Microglia (Iba1) Immunoreactivity between Wistar-Kyoto and Wistar Females

Mileva

Moyes²,

Syed³

et al. 2017

Neuropsychobiology

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Background: Depression is often associated with an increase in hypothalamic-pituitary-adrenal (HPA) axis reactivity and immune response. To investigate this relationship, we examined the consequences of environmental manipulation on the neural correlates of the HPA axis and immune response in an animal model of depression, the Wistar-Kyoto (WKY) rat. Additionally, female animals are often overlooked in preclinical research because of the hormone fluctuations inherent in the estrous cycle. Methods: Female rats were randomly assigned to 1 of 3 environments for 30 days: (1) environmental enrichment (EE), (2) standard housing (SH), and (3) isolated housing (IH). Immunoreactivity of astrocytes (glial fibrillary acidic protein [GFAP]), glucocorticoid receptors (GRs), and microglia (Iba1) in the hippocampus and amygdala were measured using immunohistochemistry. Results: WKY animals had significantly more GR staining area and Iba1 staining intensity and area in the CA1 of the hippocampus. In enriched Wistar rats, GFAP staining intensity and area were greater in the CA1. A trend towards a greater percent of area stained with GR was found in WKY animals as compared to that of the Wistar animals. This was due to WKY females in EE having significantly higher GR staining intensity and area in the amygdala as compared to that of animals in SH. Discussion: These strain differences lend support to the use of WKY animals as an animal model of depression. Furthermore, due to the effects of EE on GFAP and GR staining in WKY females, we suggest that EE can be used as an intervention to potentially alleviate the negative effects of depression.

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Section: Discussionmentioning

confidence: 99%