105 Akt signaling mediates prostate cancer response to cryoablation

Baust, John M.; Klossner, Daniel P.; Gage, Andrew A.; Buskirk, Robert Van

doi:10.1016/j.cryobiol.2013.09.111

Cited by 4 publications

(2 citation statements)

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“…It has also been shown that in addition to causing cell rupture and necrosis, freezing activates apoptosis resulting in cell death both rapid and delayed in nature. 22 , 25 – 32 …”

Section: Introductionmentioning

confidence: 99%

“…It has also been shown that in addition to causing cell rupture and necrosis, freezing activates apoptosis resulting in cell death both rapid and delayed in nature. 22,[25][26][27][28][29][30][31][32] The attractiveness of modern cryoablative therapies is related to numerous factors including a global acceptance of minimally invasive therapies, rapid adoption of intraoperative ultrasound, advances in cryosurgical device technology, and the ability to provide transient, selective protection to adjacent, nontargeted tissues. 5,10,[33][34][35][36][37][38] The key to procedural success is dosimetry in terms of temperature and duration of the freezethaw cycle.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Cryosurgical Device Performance Using a 3D Tissue-Engineered Cancer Model

Baust

Robilotto

Snyder

et al. 2017

Technol Cancer Res Treat

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As the clinical use of cryoablation for the treatment of cancer has increased, so too has the need for knowledge on the dynamic environment within the frozen mass created by a cryoprobe. While a number of factors exist, an understanding of the iceball size, critical isotherm distribution/penetration, and the resultant lethal zone created by a cryoprobe are critical for clinical application. To this end, cryoprobe performance is typically characterized based on the iceball size and temperature penetration in phantom gel models. Although informative, these models do not provide information as to the impact of heat input from surrounding tissue nor give any information on the ablative zone created. As such, we evaluated the use of a tissue-engineered tumor model (TEM) to assess cryoprobe performance including iceball size, real-time thermal profile distribution, and resultant ablative zone. Studies were conducted using an Endocare V-probe cryoprobe, with a 10/5/10 double freeze–thaw protocol using prostate and renal cancer TEMs. The data demonstrate the generation of a 33- to 38-cm3 frozen mass with the V-Probe cryoprobe following the double freeze of which ∼12.7 and 6.5 cm3 was at or below −20°C and −40°C, respectively. Analysis of ablation zone using fluorescence microscopy 24 hours postthaw demonstrated that the internal ∼40% of the frozen mass was completely ablated, whereas in the periphery of the iceball (outer 1 cm region), a gradient of partial to minimal destruction was observed. These findings correlated well with clinical reports on renal and prostate cancer cryoablation. Overall, this study demonstrates that TEMs provide an effective model for a more complete characterization of cryoablation device performance. The data demonstrate that while the overall iceball size generated in the TEM was consistent with published reports from phantom models, the integration of an external heat load, circulation, and cellular components more closely reflect an in vivo setting and the impact of penetration of the critical (−20°C and −40°C) isotherms into the tissue. This is important as it is well appreciated in clinical practice that the heat load of a tissue, cryoprobe proximity to vasculature, and so on, can impact outcome. The TEM model provides a means of characterizing the impact on ablative dose delivery allowing for a better understanding of probe performance and potential impact on ablative outcome.

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“…It has also been shown that in addition to causing cell rupture and necrosis, freezing activates apoptosis resulting in cell death both rapid and delayed in nature. 22 , 25 – 32 …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of Cryosurgical Device Performance Using a 3D Tissue-Engineered Cancer Model

Baust

Robilotto

Snyder

et al. 2017

Technol Cancer Res Treat

Self Cite

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The Story of Adjuvants to Boost the Performance of Cryoablation

Santucci

Baust

Snyder

et al. 2017

Imaging and Focal Therapy of Early Prostate Cancer

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Re-purposing cryoablation: a combinatorial ‘therapy’ for the destruction of tissue

Baust

Bischof

Jiang-Hughes

et al. 2015

Prostate Cancer Prostatic Dis

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It is now recognized that the tumor microenvironment creates a protective neo-tissue that isolates the tumor from the various defense strategies of the body. Evidence demonstrates that, with successive therapeutic attempts, cancer cells acquire resistance to individual treatment modalities. For example, exposure to cytotoxic drugs results in the survival of approximately 20-30% of the cancer cells as only dividing cells succumb to each toxic exposure. With follow-up treatments, each additional dose results in tumor-associated fibroblasts secreting surface-protective proteins, which enhance cancer cell resistance. Similar outcomes are reported following radiotherapy. These defensive strategies are indicative of evolved capabilities of cancer to assure successful tumor growth through well-established anti-tumor-protective adaptations. As such, successful cancer management requires the activation of multiple cellular 'kill switches' to prevent initiation of diverse protective adaptations. Thermal therapies are unique treatment modalities typically applied as monotherapies (without repetition) thereby denying cancer cells the opportunity to express defensive mutations. Further, the destructive mechanisms of action involved with cryoablation (CA) include both physical and molecular insults resulting in the disruption of multiple defensive strategies that are not cell cycle dependent and adds a damaging structural (physical) element. This review discusses the application and clinical outcomes of CA with an emphasis on the mechanisms of cell death induced by structural, metabolic, vascular and immune processes. The induction of diverse cell death cascades, resulting in the activation of apoptosis and necrosis, allows CA to be characterized as a combinatorial treatment modality. Our understanding of these mechanisms now supports adjunctive therapies that can augment cell death pathways.

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105 Akt signaling mediates prostate cancer response to cryoablation

Cited by 4 publications

References 0 publications

Assessment of Cryosurgical Device Performance Using a 3D Tissue-Engineered Cancer Model

Assessment of Cryosurgical Device Performance Using a 3D Tissue-Engineered Cancer Model

The Story of Adjuvants to Boost the Performance of Cryoablation

Re-purposing cryoablation: a combinatorial ‘therapy’ for the destruction of tissue

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