Abstract:BackgroundFibroleukin or fibrinogen-like protein-2 is a immunomodulatory protein that was described to bind to Fcgamma-receptor-IIb and III. In the present study the effects of a fusion protein consisting of fibroleukin and the major allergen of shrimp, tropomyosin, on human basophilic responses were investigated in vitro.MethodsThe fusion molecule was generated by molecular cloning and expressed in E. coli. Receptor binding studies were performed by immunoblot, ELISA, and flow cytometry. Activation of basophi… Show more
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