103. Dysregulated circulating microRNAs in preeclampsia: The role of miR-574-5p and miR-1972 in endothelial dysfunction

Lip, Simone V.; Boekschoten, Mark V.; Pampus, Maria van; Scherjon, Sicco A.; Plösch, Torsten; Faas, Marijke M.

doi:10.1016/j.preghy.2018.08.068

Cited by 4 publications

(3 citation statements)

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“…Bta-miR-574 exhibited a loss of gene connectivity in the SFH group. Interestingly, miR-574 was identified to be upregulated in women with preeclampsia and involved with endothelial dysfunction ( Lip et al, 2018 ). Furthermore, miR-574 in porcine cumulus cells has been shown to suppress oocyte maturation ( Pan et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential

et al. 2023

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Reproductive performance is the most critical factor affecting production efficiency in the cow-calf industry. Heifers with low reproductive efficiency may fail to become pregnant during the breeding season or maintain a pregnancy. The cause of reproductive failure often remains unknown, and the non-pregnant heifers are not identified until several weeks after the breeding season. Therefore, improving heifer fertility utilizing genomic information has become increasingly important. One approach is using microRNAs (miRNA) in the maternal blood that play an important role in regulating the target genes underlying pregnancy success and thereby in selecting reproductively efficient heifers. Therefore, the current study hypothesized that miRNA expression profiles from peripheral white blood cells (PWBC) at weaning could predict the future reproductive outcome of beef heifers. To this end, we measured the miRNA profiles using small RNA-sequencing in Angus-Simmental crossbred heifers sampled at weaning and retrospectively classified as fertile (FH, n = 7) or subfertile (SFH, n = 7). In addition to differentially expressed miRNAs (DEMIs), their target genes were predicted from TargetScan. The PWBC gene expression from the same heifers were retrieved and co-expression networks were constructed between DEMIs and their target genes. We identified 16 differentially expressed miRNAs between the groups (p-value ≤0.05 and absolute (log2 fold change ≥0.05)). Interestingly, based on a strong negative correlation identified from miRNA-gene network analysis with PCIT (partial correlation and information theory), we identified miRNA-target genes in the SFH group. Additionally, TargetScan predictions and differential expression analysis identified bta-miR-1839 with ESR1, bta-miR-92b with KLF4 and KAT2B, bta-miR-2419-5p with LILRA4, bta-miR-1260b with UBE2E1, SKAP2 and CLEC4D, and bta-let-7a-5p with GATM, MXD1 as miRNA-gene targets. The miRNA-target gene pairs in the FH group are over-represented for MAPK, ErbB, HIF-1, FoxO, p53, mTOR, T-cell receptor, insulin and GnRH signaling pathways, while those in the SFH group include cell cycle, p53 signaling pathway and apoptosis. Some miRNAs, miRNA-target genes and regulated pathways identified in this study have a potential role in fertility; other targets are identified as novel and need to be validated in a bigger cohort that could help to predict the future reproductive outcomes of beef heifers.

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Section: Discussionmentioning

confidence: 99%

miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential

et al. 2023

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“…The tube formation assay is a well-established model for measuring formation of endothelial cell tubes, which is part of the angiogenesis process in vitro 1 . The other two assays also assess processes that are important for angiogenesis 2 .…”

Section: Study Design and Rationalmentioning

confidence: 99%

“…the ability of the endothelial cells to form capillary-like structures in vitro. This is part of the angiogenic process 1 . Matrigel basement membrane matrix (Corning) was defrosted at 4°C overnight.…”

Section: Tube Formation Assaymentioning

confidence: 99%

Early-onset preeclampsia, plasma microRNAs, and endothelial cell function

Lip

Boekschoten

Hooiveld

et al. 2020

American Journal of Obstetrics and Gynecology

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Expression profile of plasma microRNAs and target genes in patients with complicated pregnancy

Pashkovsky

Solovieva

Rabadanova

et al. 2023

Akušerstvo, ginekologiâ i reprodukciâ

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Aim: comparative analysis of the expression profile of plasma microRNAs and target genes in patients with complicated pregnancy.Materials and Methods. A prospective observational comparative study in parallel groups was carried out. The study included 73 women divided into three groups: the main group – 42 patients with preeclampsia (PE), the comparison group – 12 pregnant women with fetal growth retardation (FGR), the control group – 19 clinically healthy women with uncomplicated pregnancy. An examination was performed, which included the analysis of clinical characteristics and the study of microRNA expression in blood plasma using the real-time polymerase chain reaction.Results. MicroRNA hsa-miR-210-5p and hsa-miR-1972 were not identified in any plasma sample. Analyzing plasma microRNAs in group of women with PE showed significant changes in the expression levels of hsa-miR-517a-3p (p = 0.025), hsa-miR-517c-3p (p = 0.036), hsa-miR-574-5p (p = 0.015), hsa-miR-517a-3p (p < 0.001) and an increase in miR-20a-5p (p = 0.046) compared to control group. No significant differences were found in the miRNA expression profile in group of women with FGR compared to control group. Assessing an influence of the studied microRNAs on regulatory signaling pathways allowed to establish that hsa-miR-miR-146a-5p, -181a-5p, -210-3p, -517a-3p, -517c-3p, -574-3p, -574-5p, -1304-5p are potential regulators of the reaction cascades involved in the PE pathogenesis.Conclusion. The changes revealed in the circulating blood plasma miRNA level indicate the presence of specific transcriptomic alterations during complicated course of pregnancy.

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103. Dysregulated circulating microRNAs in preeclampsia: The role of miR-574-5p and miR-1972 in endothelial dysfunction

Cited by 4 publications

References 0 publications

miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential

miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential

Early-onset preeclampsia, plasma microRNAs, and endothelial cell function

Expression profile of plasma microRNAs and target genes in patients with complicated pregnancy

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