1020 MHz single-channel proton fast magic angle spinning solid-state NMR spectroscopy

Abstract

This study reports a first successful demonstration of a single channel proton 3D and 2D high-throughput ultrafast magic angle spinning (MAS) solid-state NMR techniques in an ultra-high magnetic field (1020 MHz) NMR spectrometer comprised of HTS/LTS magnet. High spectral resolution is well demonstrated.

1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

“…This brings into SSNMR2324252627282930313233343536 many of the procedures for assignment and collection of structural restraints routinely applied in solution NMR since several years37. Higher fields and faster spinning made it even possible to record 1 H- 1 H correlation experiments38. Recently, 1 H-detection based experiments were applied to the study of bone39.…”

NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology

“…With this magnet, they have demonstrated the ability to acquire high-quality natural abundance spectra for insensitive nuclei such as 17 O (0.04% natural abundance (1, 23)). 1 H- 1 H- 1 H 3D data indicate the field homogeneity and stability achieved at 24 T (24). As discussed below, a significant advantage of magnetic field strengths over 1 GHz is in the measurement of chemical shift anisotropies (CSAs).…”

“…tumbling) sample. Given the relatively small magnitude of the 1 H CSA tensor and the B 0 field dependence of the CSA interaction, executing these measurements at higher magnetic fields allows for increased resolution and accuracy of measurements (24). With the development of ultrahigh magnetic fields, use of 1 H CSA tensors may become a routine parameter for use in protein structure determination by NMR.…”

“…This is particularly advantageous for small CSAs, such as for the aliphatic protons. Pandey et al demonstrated the measurement of 1 H CSAs for L-histidine with the dual LTS/HTS 24 T spectrometer (24). …”

Biomolecular NMR: Past and future

“…19 F-labeling of proteins appears poised for a renaissance [109]. Solid state NMR with ultra-fast magic angle spinning [94] and signal enhancement by DNP [106] are expected to continue their rapid development. The seamless interoperability of biomolecular NMR software packages under a virtual machine [54] is likely to become more refined through the NMRbox project (https://www.nmrbox.org/).…”