102 Immunohistological detection of parvovirus B19 VP1- and VP2-capsid proteins in endomyocardial biopsies from dilated cardiomyopathy patients

Escher, Felicitas; Kuehl, Uwe; Laßner, Dirk; Suckau, Lennart; Poller, Wolfgang; Schultheiss, H.‐P.; Noutsias, Michel

doi:10.1016/s1567-4215(07)60064-2

Cited by 10 publications

(11 citation statements)

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“…The relevance of B19V genome detection in AMC is controversial, so far, due to the inability to differentiate cases with acute, replicating B19V infections from cases with asymptomatic, lifelong B19V persistence by the mere nested PCR (nPCR) amplification of B19V genomes in EMBs 4,37. Potentially important additional investigations such as B19V protein expression and the differentiation of anti‐B19V humoral response patterns37,38 were not available in these patients. Data for HHV6 associated TRBV dominance in myocarditis are not available.…”

Section: Discussionmentioning

confidence: 99%

Expression of functional T-cell markers and T-cell receptor Vbeta repertoire in endomyocardial biopsies from patients presenting with acute myocarditis and dilated cardiomyopathy

Noutsias

Rohde

Göldner

et al. 2011

European Journal of Heart Failure

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Aims To quantify and functionally characterize the intramyocardial T‐cells in endomyocardial biopsies (EMBs) from patients presenting with acute myocarditis (AMC) and dilated cardiomyopathy (DCM). Methods and results Expression of genes characterizing Th1 [interferon (IFN)γ, Tbet‐1, Eomesodermin, interleukin (IL)‐27], Th2 (IL‐4, IL‐5, GATA3), Th17 (IL‐17), regulatory [regulatory T‐cells (Treg); FoxP3, TGFβ, IL‐10], anergic (GRAIL), and cytotoxic T‐cells (CTLs: Perforin, Granulysin, Granzyme A), as well as of functional T‐cell receptor Vbeta (TRBV) families were investigated in EMBs from AMC patients (n= 58) and DCM patients (n= 34) by pre‐amplified real‐time reverse transcription‐polymerase chain reaction. These data were compared with EMBs from n= 19 controls. Expression of CD3d, CD3z, and TRBC (T‐cell receptor beta constant region) were associated with the immunohistological diagnosis of inflammatory cardiomyopathy (DCMi). In EMBs from DCM patients with increased CD3d expression, significantly increased markers of Th1 (IFNγ, T‐bet, Eomesodermin), regulatory T‐cells (Treg; FoxP3, TGFβ), and cytotoxic T‐cells (CTLs: Perforin, Granulysin, Granzyme A) were present, while no differential polarization of T‐cells was found in EMBs form AMC patients. A differential dominance of distinct functional TRBV families was associated with different cardiotropic viruses: TRBV 11 and 24 with Parvovirus B19; TRBV4, 10 and 28 with human herpes virus type 6; and TRBV14 for Coxsackie virus, respectively. Conclusions The T‐cell infiltrates in human DCMi are characterized by differential expression of functional T‐cell markers indicating Th1, Treg, and CTLs, while no major role could be confirmed for Th17. The virus‐associated differential TRBV dominance suggests an antiviral specificity of virus‐induced T‐cell responses in human DCMi.

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Section: Discussionmentioning

confidence: 99%

Expression of functional T-cell markers and T-cell receptor Vbeta repertoire in endomyocardial biopsies from patients presenting with acute myocarditis and dilated cardiomyopathy

Noutsias

Rohde

Göldner

et al. 2011

European Journal of Heart Failure

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“…The coded slides were examined in a blinded fashion. Immunohistological staining was carried out and the immunoreactivity was quantified by digital image analysis (unit: area fraction) at 200-fold magnification as described in detail elsewhere 12 13…”

Section: Methodsmentioning

confidence: 99%

Fractalkine in human inflammatory cardiomyopathy

Escher

Vetter

Kühl

et al. 2011

Heart

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“…The coded slides were examined in a blinded fashion. Immunohistological staining was carried out and the immunoreactivity was quantified by digital image analysis (unit:area fraction) at 200-fold magnification as described in detail elsewhere 14 15…”

Section: Methodsmentioning

confidence: 99%

Development of diastolic heart failure in a 6-year follow-up study in patients after acute myocarditis

Escher

Westermann

Gaub

et al. 2010

Heart

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Background The aim of this study was to analyse the long-term prognosis of patients with acute myocarditis (AMC) who had been discharged from hospital while having normal left ventricular (LV) function. Methods and results 50 patients with acute myocarditis who underwent endomyocardial biopsies (EMBs) were prospectively studied. Their clinical condition was examined during a mean follow-up period of 72 (54e78) months, including tissue Doppler imaging (TDI). 4% (2/50) died, and 6% (3/50) developed dilated cardiomyopathy. 45/50 (90%) showed a normal or improvement in LV function over time. In the course of the follow-up, 49% (22/45) suffered from heart failure symptoms despite a normal ejection fraction (HFNEF). This was associated with an abnormal E/A ratio, an impaired deceleration time of early mitral flow velocity and isovolumic relaxation time, and a pathological increase in the LV filling index E/E 9

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102 Immunohistological detection of parvovirus B19 VP1- and VP2-capsid proteins in endomyocardial biopsies from dilated cardiomyopathy patients

Cited by 10 publications

References 0 publications

Expression of functional T-cell markers and T-cell receptor Vbeta repertoire in endomyocardial biopsies from patients presenting with acute myocarditis and dilated cardiomyopathy

Expression of functional T-cell markers and T-cell receptor Vbeta repertoire in endomyocardial biopsies from patients presenting with acute myocarditis and dilated cardiomyopathy

Fractalkine in human inflammatory cardiomyopathy

Development of diastolic heart failure in a 6-year follow-up study in patients after acute myocarditis

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