CircularRNA_0119872 regulates the microRNA-582-3p/E2F transcription factor 3 pathway to promote the progression of malignant melanoma

Qu, Jinlong; Yuan, Chunying; Jia, Qi; Sun, Ming; Jiang, Min; Zuo, Fuguang

doi:10.6061/clinics/2021/e3036

Cited by 4 publications

(6 citation statements)

References 24 publications

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“…Mechanistically, miRNAs usually were targeted by circRNAs and then regulated mRNA to influence melanoma progression. Circ_0119872 acted as a sponge for miR‐582‐3p, and E2F3 acted as a target gene of miR‐582‐3p, this axis promoted melanoma proliferation and metastasis 12 . Circ_0082835 upregulated EZH2 by targeting miR‐429 and promoted the development of melanoma 24 .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, there is growing evidence that circRNAs are important processes in cancer cell viability, metastasis, or glycolysis 7,8 . The involvement of circRNA in the pathogenesis of tumors including melanoma has become a hot topic 9–12 . For example, circ_0062270 regulated miR‐331‐3p/EPHA2 axis thus promoted melanoma development 9 .…”

Section: Introductionmentioning

confidence: 99%

“…[15][16][17] Multiple reports have pointed out that some miRNAs participate in melanoma progression and have the potential as novel biomarkers for melanoma. [18][19][20] Given the huge effect of circRNA-miRNA interaction on cancer malignant progression, 9,12 we analyzed the target miRNAs of circ_0081054. As predicted by online databases circBank and circular RNA interactome, circ_0081054 potentially bound to miR-1204, miR-637, miR-1225-3p, miR-875-5p.…”

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confidence: 99%

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Circ_0081054 facilitates melanoma development via sponging miR‐637 and regulating RAB9A

Kong

et al. 2023

Skin Research and Technology

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Background: Accumulating evidence announces that aberrantly expressed circRNAs were closely related to the development of human cancers. However, the role and mechanism of multiple circRNAs remain unclear. Our work aimed to disclose the functional role and mechanism of circ_0081054 in melanoma. Methods:Quantitative real-time polymerase chain reaction assay was utilized to detect circ_0081054, microRNA-637 (miR-637) and RAB9A (member RAS oncogene family) mRNA expression. Cell proliferative ability was evaluated via Cell Counting Kit-8 and colony formation assay. Cell invasion was assessed by using wound healing assay. Results:The significant upregulation of circ_0081054 was detected in melanoma tissues and cells. The proliferation, migration, glycolytic metabolism, and angiogenesis in melanoma cells were suppressed, while apoptosis was promoted following the silence of circ_0081054. In addition, circ_0081054 could target miR-637, and miR-637 inhibitor could reverse the effects of circ_0081054 deficiency. Furthermore, RAB9A was a target gene for miR-637 and RAB9A overexpression could reverse the effects of miR-637 overexpression. In addition, the deficiency of circ_0081054 hampered tumor growth in vivo. Moreover, circ_0081054 could regulate RAB9A expression by sponging miR-637. Conclusion:All results indicated that circ_0081054 promoted the malignant behaviors of melanoma cells partly by regulating the miR-637/RAB9A molecular axis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Circ_0081054 facilitates melanoma development via sponging miR‐637 and regulating RAB9A

Kong

et al. 2023

Skin Research and Technology

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“…CircRNAs that have binding sites for miRNAs may act as sponges for miRNAs to play regulatory roles (Qu et al 2021). In addition, more and more studies had demonstrated that miRNAs regulated the expression of downstream target genes (Bi et al 2019;, and circRNAs further regulated the expression changes of downstream genes through miRNAs (Bi et al 2019).…”

Section: Discussionmentioning

confidence: 99%

High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis

Li¹,

Gao²,

Zhao³

et al. 2022

Biochem Genet

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It has been reported that circRNAs play an important regulatory role in the progression of colorectal cancer (CRC). However, the molecular role of circ_0001821 in CRC development is unclear. In this study, we aimed to investigate the regulatory role and molecular mechanisms of circ_0001821 in CRC. Reverse transcription-quantitative PCR and western blot assays were used to detect the expression of circ_0001821, miR-600 and isochorismatase domain containing 1 (ISOC1) in CRC tissues as well as its cell lines. Colony formation assay and EDU assay were used to detect the proliferative capacity of cells. Transwell assay was used to assess cell migration and invasion ability. Flow cytometry was used to analyze cell apoptosis. ELISA was used to measure the glycolytic capacity of cells. Dual-luciferase reporter assay and RNA pull-down assay were used to analyze the relationships between circ_0001821, miR-600 and ISOC1. Animal experimentation was used to validate the functional study of circ_0001821 in vivo. Immunohistochemistry (IHC) of Ki67 staining analysis was conducted to assess tumor growth. Circ_0001821 and ISOC1 were significantly increased in CRC tissues and its cell lines, and miR-600 was significantly decreased in CRC tissues and its cell lines. Silencing circ_0001821 inhibited cell proliferation, migration, invasion and glycolytic capacity, while inducing apoptosis. And it could inhibit tumor growth in vivo. Circ_0001821 could act as a sponge for miR-600 to regulate CRC processes. ISOC1 was identified as a downstream regulator of miR-600, also miR-600 could regulate the expression of ISOC1. In addition, circ_0001821 could regulate ISOC1 expression changes through miR-600. Mechanistically, either miR-600 inhibitor or overexpression of ISOC1 could reverse the effects of knockdown of circ_0001821 on cell biological properties. Circ_0001821 regulated the developmental process of CRC through miR-600/ISOC1 axis.

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“… 74 In malignant melanoma, circ_0119872 encourages cell proliferation by relieving the inhibitory effect of miR-582-3p on E2F3 via sponging. 75 Another study reported that circAGAP1 (circ0058792) expression was significantly upregulated in clear cell renal cell carcinoma (ccRCC) tissues compared to that in adjacent non-tumor tissues. In particular, circAGAP1 played a carcinogenic role in ccRCC progression by regulating the miR-15-5p/E2F3 axis.…”

Section: Circrna-mediated Regulation Of Cell Cycle Via Prb-e2fsmentioning

confidence: 99%

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

Liu¹,

Qu²,

Yang³

et al. 2022

OTT

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Circular RNAs (circRNAs) participate in the occurrence and development of various diseases through different mechanisms, such as by acting as a microRNA (miRNA) sponge, interacting with RNA-binding proteins, and regulating gene transcription and protein translation. For example, the abnormal expression of specific circRNAs in tumor cells can alter key regulatory factors and the cell cycle network, resulting in cell cycle disorders and the development and metastasis of tumors. Here, we summarize the mechanisms involved in the circRNA-mediated processes that lead to uncontrolled cell cycle and tumor cell proliferation. Extensive studies investigating the abnormal expression of circRNAs in different cancer types have been conducted. The unique characteristics of circRNAs and their ability to regulate the cell cycle through diverse mechanisms is extremely valuable in tumor diagnosis, treatment, and prognosis. Our review may assist in further understanding the circRNA-mediated regulation of the cell cycle in tumors and provide insights for research on circRNA-based therapeutic strategies and biological diagnosis for cancer.

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CircularRNA_0119872 regulates the microRNA-582-3p/E2F transcription factor 3 pathway to promote the progression of malignant melanoma

Cited by 4 publications

References 24 publications

Circ_0081054 facilitates melanoma development via sponging miR‐637 and regulating RAB9A

Circ_0081054 facilitates melanoma development via sponging miR‐637 and regulating RAB9A

High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

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