Overexpression of serum extracellular vesicle microRNA-215-5p is associated with early tumor recurrence and poor prognosis of gastric cancer

Zhang, Yun‐Fei; Huang, Fengchang; Xu, Ning; Wang, Jin; Li, Dan; Yin, Liang

doi:10.6061/clinics/2021/e2081

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…2017 [ 57 ] China NA miR-665 HCC qRT-PCR ExoQuick kit serum 30 60 12/18 I—IV OS E NA NA NA NA poor Reese M. 2020 [ 58 ] Germany 2015–2018 miR-200b miR-200c PDAC qRT-PCR UC serum 56 60 36/20 I—IV OS miR-200b OS: R-Multi/ R-Uni miR-200c OS: R-Multi/ R-Uni 13 NA NA 0.790 (0.680–0.890) 0.670 (0.550–0.790) good Meltzer S. 2019 [ 59 ] Norway NA miR-141-3p miR-375 LARC qRT-PCR Exosome isolation kit plasma 64 60 36/28 NA OS, PFS OS: R-Uni PFS: R-Uni 65 (range 4–66) NA NA NA good Wang Q. 2021 [ 60 ] China NA miR-1290 HCC qRT-PCR …”

Section: Resultsmentioning

confidence: 99%

“…2021 [ 60 ] China NA miR-1290 HCC qRT-PCR Exosome isolation kit/UC serum 49 55 37/12 I—IV NA NA NA NA NA NA poor Zhang Y. 2021 [ 60 ] China NA miR-215-5p GC qRT-PCR ExoQuick kit serum 118 60 70/48 I—IV OS, DFS OS: R-Multi/ R-Uni DFS: E NA 97.1 68.6 0.866 good Zhu L. 2022 [ 61 ] China 2020–2021 miR-552-5p GC qRT-PCR UC plasma 30 56 16/14 I—IV NA NA NA NA NA NA good Yang J. 2022 [ 62 ] China NA miR-195-5p miR-211-5p GC RT-qPCR UC plasma 108 …”

Section: Resultsmentioning

confidence: 99%

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Circulating exosomal microRNAs as potential prognostic biomarkers in gastrointestinal cancers: a systematic review and meta-analysis

et al. 2023

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Background Recent reports suggested that circulating exosomal microRNAs (exomiRs) may serve as non-invasive prediction biomarkers in gastrointestinal (GI) cancers, yet their clinicopathological and prognostic values need to be more clarified. Hence, the present meta-analysis was aimed to quantitatively assess the evidence regarding the association between circulating exomiRs and prognosis in GI cancer patients. Methods A comprehensive search was carried out in prominent literature databases, including PubMed, ISI Web of Science, Scopus, and Embase. Odds ratios (ORs) or hazard ratios (HRs) with 95% confidence intervals (CIs) were gathered to evaluate the strength of the association. The quality assessment was investigated through the Newcastle-Ottawa Scale (NOS) and publication bias via Eggers’ test and funnel plots. Results A total of 47 studies, comprising of 4881 patients, were considered eligible for this meta-analysis. Both up-regulated and down-regulated circulating exomiRs are significantly associated with differentiation (HR = 1.353, P = 0.015; HR = 1.504, P = 0.016), TNM stage (HR = 2.058, P < 0.001; HR = 2.745, P < 0.001), lymph node metastasis (HR = 1.527, P = 0.004; HR = 2.009, P = 0.002), distant metastasis (HR = 2.006, P < 0.001; HR = 2.799, P = 0.002), worse overall survival (OS) (HR = 2.053, P < 0.001; HR = 1.789, P = 0.001) and poorer disease/relapse/progression-free survival (DFS/RFS/PFS) (HR = 2.086, P < 0.001; HR = 1.607, P = 0.001) in GI cancer patients, respectively. In addition, subgroup analyses based on seven subcategories indicated the robustness of the association. The majority of findings were lack of publication bias except for the association between up-regulated exomiRs and OS or DFS/RFS/PFS and for the down-regulated exomiRs and TNM stage. Conclusion This study supports that up- and down-regulated circulating exomiRs are associated with poorer survival outcomes and could be served as potential prognostic biomarkers in GI cancers. Given the limitations of the current findings, such as significant heterogeneity, more investigations are needed to fully clarify the exomiRs prognostic role.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Circulating exosomal microRNAs as potential prognostic biomarkers in gastrointestinal cancers: a systematic review and meta-analysis

et al. 2023

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Extracellular vesicles associated microRNAs: Their biology and clinical significance as biomarkers in gastrointestinal cancers

Li,

Sui,

Goel

2024

Seminars in Cancer Biology

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Plasma miRNA expression in patients with genetically confirmed multiple endocrine neoplasia type 1 syndrome and its phenocopies

Trukhina,

Mamedova,

Nikitin

et al. 2024

Probl Endokrinol (Mosk)

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BACKGROUND: MEN-1 is a rare autosomal dominant disease caused by mutations in MEN1 gene encoding the menin protein. This syndrome is characterized by the occurrence of parathyroid tumors, gastroenteropancreatic neuroendocrine tumors, pituitary adenomas, as well as other endocrine and non-endocrine tumors. If a patient with the MEN-1 phenotype carry no mutations in the MEN1 gene, the condition considers a phenocopy of syndrome (phMEN1). The possible cause of this changes could be changes in epigenetic regulation, particularly in microRNA expression that might affect menin signaling pathways.AIM: to identify differently expressed circulating miRNAs in plasma in patients with genetically confirmed MEN-1 syndrome, its phenocopies and healthy controls.MATERIALS AND METHODS: single-center, case-control study was conducted. We assessed plasma microRNA expression in patients with genetically confirmed MEN-1 (gMEN1), phMEN1 and healthy controls. Morning plasma samples were collected from fasting patients and stored at –80°C. Total RNA isolation was performed using miRNeasy Mini Kit with QIAcube. The libraries were prepared by the QIAseq miRNA Library Kit following the manufacturer. Circulating miRNA sequencing was done on Illumina NextSeq 500 (Illumina). Subsequent data processing was performed using the DESeq2 bioinformatics algorithm.RESULTS: we enrolled 21 consecutive patients with gMEN1 and 11 patients with phMEN1, along with 12 gender matched controls. Median age of gMEN1 was 38,0 [34,0; 41,0]; in phMEN1 — 59,0 [51,0; 60,0]; control — 59,5 [51,5; 62,5]. The gMEN1 group differed in age (p<0.01) but not gender (р=0.739) or BMI (р=0.116) compared to phMEN1 and controls group, the last two groups did not differ by these parameters (p>0.05). 25 microRNA were differently expressed in groups gMEN1 and phMEN1 (21 upregulated microRNAs, 4 — downregulated). Comparison of samples from the phMEN-1 group and relatively healthy controls revealed 10 differently expressed microRNAs: 5 — upregulated; 5 — downregulated. In the gMEN-1 and control groups, 26 differently expressed microRNAs were found: 24 — upregulated; 2 — downregulated. The miRNAs most differing in expression among the groups were selected for further validation by RT-qPCR (in the groups of gMEN1 vs phMEN1 — miR-3613-5p, miR-335-5p, miR-32-5p, miR-425-3p, miR-25-5p, miR-576-5p, miR-215-5p, miR-30a-3p, miR-141-3p, miR-760, miR-501-3p; gMEN1 vs control — miR-1976, miR-144-5p miR-532-3p, miR-375; as well as in phMEN1 vs control — miR-944, miR-191-5p, miR-98-5p).CONCLUSION: In a pilot study, we detected microRNAs that may be expressed differently between patients with gMEN-1 and phMEN-1. The results need to be validated using different measurement method with larger sample size.

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Overexpression of serum extracellular vesicle microRNA-215-5p is associated with early tumor recurrence and poor prognosis of gastric cancer

Cited by 4 publications

References 22 publications

Circulating exosomal microRNAs as potential prognostic biomarkers in gastrointestinal cancers: a systematic review and meta-analysis

Circulating exosomal microRNAs as potential prognostic biomarkers in gastrointestinal cancers: a systematic review and meta-analysis

Extracellular vesicles associated microRNAs: Their biology and clinical significance as biomarkers in gastrointestinal cancers

Plasma miRNA expression in patients with genetically confirmed multiple endocrine neoplasia type 1 syndrome and its phenocopies

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