Screening of Strongyloides infection using an ELISA test in transplant candidates

Abstract

OBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti- Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates.

Immune complexes as a tool for strongyloidiasis immunodiagnosis in kidney and liver transplant candidate

“…This may suggest that antibody production occurred even in immunocompromises caused by the underlying disease or drugs administered in the treatments. Other studies have also demonstrated the detection of anti‐ Strongyloides IgG antibodies in immunocompromised patients such as HIV and HTLV carriers, 22 diabetics, 23 ethylists, 24 and transplant candidate patients 25 …”

“…Other studies have also demonstrated the detection of anti-Strongyloides IgG antibodies in immunocompromised patients such as HIV and HTLV carriers, 22 diabetics, 23 ethylists, 24 and transplant candidate patients. 25 The combination of data for IC and anti-IgG antibodies was determined (Figure 2). In KT candidates, the correlation was r = .2691 (p > .05).…”

Seroprevalence and microscopy detection rates of strongyloidiasis in Croatian patients with eosinophilia

“…This is particularly pertinent when corticosteroid therapy is prescribed, as previously undetected and/or subclinical Strongyloides infestation can develop into hyperinfection syndrome during treatment and subsequently disseminate, leading to very high mortality rates (Ahmed et al , 2019). Another important group where serological evaluation should be routinely sought are transplantation candidates (Toledo et al , 2019), although reduced sensitivity of serology in immunocompromised individuals should be taken into account. A study by Winnicki et al (2018) suggests that kidney transplantation programmes in Central Europe should entail recipient and donor screening as Strongyloides may be underestimated.…”

Diagnostic accuracy of somatic and excretory−secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

“…Detection of serum anti-Strongyloides IgG antibodies was performed by ELISA based on the procedure described by Toledo et al (2019). The test was previously standardized by checkerboard titration using different concentrations of the three antigenic preparations (0.5, 1, 5, 10 and 20 μg/mL) and different dilutions for serum samples (1 : 50, 1 : 100, 1 : 200, 1 : 300 and 1 : 400) and for conjugate secondary antibody (1 : 10 000, 1 : 20 000, 1 : 30 000, 1 : 40 000, and 1 : 50 000).…”