Proposal for a New Histological Scoring System for Cartilage Repair

Ponce, Maria Clara; Zorzi, Alessandro; Miranda, João Batista de; Amstalden, Eliane Maria Ingrid

doi:10.6061/clinics/2018/e562

Cited by 6 publications

(5 citation statements)

References 19 publications

(39 reference statements)

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“…In past studies, researchers have gradually found that, although tissue scaffolds made by general manufacturing techniques can alter the extracellular environment, the changes are more uniform and largely uncontrollable (Teimouri et al, 2015;Zhang et al, 2017;Ponce et al, 2018;Li et al, 2019). However, the complex environment in normal tissues is significantly different.…”

Section: Introductionmentioning

confidence: 99%

Effect of Pore Size on Cell Behavior Using Melt Electrowritten Scaffolds

Han

Lian

et al. 2021

Front. Bioeng. Biotechnol.

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Tissue engineering technology has made major advances with respect to the repair of injured tissues, for which scaffolds and cells are key factors. However, there are still some issues with respect to the relationship between scaffold and cell growth parameters, especially that between the pore size and cells. In this study, we prepared scaffolds with different pore sizes by melt electrowritten (MEW) and used bone marrow mensenchymal stem cells (BMSCs), chondrocytes (CCs), and tendon stem cells (TCs) to study the effect of the scaffold pore size on cell adhesion, proliferation, and differentiation. It was evident that different cells demonstrated different adhesion and proliferation rates on the scaffold. Furthermore, different cell types showed differential preferences for scaffold pore sizes, as evidenced by variations in cell viability. The pore size also affected the differentiation and gene expression pattern of cells. Among the tested cells, BMSCs exhibited the greatest viability on the 200-μm-pore-size scaffold, CCs on the 200- and 100-μm scaffold, and TCs on the 300-μm scaffold. The scaffolds with 100- and 200-μm pore sizes induced a significantly higher proliferation, chondrogenic gene expression, and cartilage-like matrix deposition after in vitro culture relative to the scaffolds with smaller or large pore sizes (especially 50 and 400 μm). Taken together, these results show that the architecture of 10 layers of MEW scaffolds for different tissues should be different and that the pore size is critical for the development of advanced tissue engineering strategies for tissue repair.

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Section: Introductionmentioning

confidence: 99%

Effect of Pore Size on Cell Behavior Using Melt Electrowritten Scaffolds

Han

Lian

et al. 2021

Front. Bioeng. Biotechnol.

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“…This structure can regenerate and form cartilage tissue; however, it is uncertain which cell is responsible for this differentiation. 12,22…”

Section: Discussionmentioning

confidence: 99%

“…The matrix in adjacent islands stained in blue and was considered newly formed cartilage as the elastic cartilage has the ability to regenerate from the perichondrium; the bluish stain indicates newly formed collagen. 8,22 The perichondrium is a structure that surrounds the cartilage and is composed of an external, vascularized layer of loose connective tissue, surrounding a fibrous zone and a subchondral zone that fuses with the cartilage matrix. This structure can regenerate and form cartilage tissue; however, it is uncertain which cell is responsible for this differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…This structure can regenerate and form cartilage tissue; however, it is uncertain which cell is responsible for this differentiation. 12,22 In the sampled pigs, the lesions were restricted to the auricular cartilages and 2 of the laryngeal cartilages (epiglottis and arytenoid). In these cases, Verhoeff's stain demonstrated the elastic nature of these cartilages.…”

Section: Discussionmentioning

confidence: 99%

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Auricular and laryngeal chondritis in nursery and finishing pigs

et al. 2023

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This work aimed to characterize the clinic-pathological presentation of an outbreak of auricular and laryngeal chondritis in pigs. Visits were made to pig farms, where the clinical history was obtained, and clinical and postmortem examinations were performed. In those farms, 3% to 4% of pigs presented otohematomas, which started in the nursery and extended to the finishing phase. Moreover, some finishing pigs presented with respiratory distress, initially characterized as inspiratory dyspnea, associated by an uncommon respiratory stridor and culminating in death. Grossly, nursery piglets had enlarged ears, and on the cut surface, the cartilage was fragmented and associated with blood clots. In the finishing phase, in addition to auricular lesions, the epiglottis and arytenoid cartilages were thickened and distorted, which partially occluded the lumen. Microscopically, the laryngeal and auricular cartilages were fragmented, displayed a loss of matrix basophilia, and were surrounded by lymphohistiocytic inflammatory infiltrate, with occasional multinucleated giant cells and fibrosis. The lesions exclusively affected elastic cartilages. The disease in finishing pigs led to increased mortality and was a differential diagnosis to respiratory challenges. It was not possible to determine the factor that triggered this condition; however, a nutritional association is suspected. To the authors’ knowledge, this is the first report of primary auricular and laryngeal chondritis in pigs.

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“…The criteria for quantitative evaluation of the histology of newly formed bone were modified from previous publications [17,18]. Briefly, stained sections were examined with a slide scanner (ScanScope ® XT, Leica Biosystems Imaging, Inc. USA).…”

Section: Quantitative Histological Evaluation Of New Bone Formationmentioning

confidence: 99%

Development of injectable chitosan/biphasic calcium phosphate bone cement and in vitro and in vivo evaluation

et al. 2020

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Injectable biphasic calcium phosphate bone cements (BCPCs) composed of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) have been intensively investigated because of their high rate of biodegradation, bioactivity and osteoconductivity, which can be adjusted by changing the ratio between β-TCP and HA phases after setting. The aim of this study was to evaluate the performance of 1 wt% chitosan fiber additive with biphasic calcium phosphate as an injectable bone cement both in vitro and in vivo. In vitro evaluation of compressive strength, degradation rate, morphology, and cell and alkaline phosphatase activities was done by comparison with bone cement without β-TCP. The in vivo results for micro-CT scanning and histological examinations for three groups (control, BCPC and commercial biphasic calcium phosphate granules) were characterized and compared. After the addition of 20 wt% β-TCP to calcium phosphate cement, the initial and final setting times of the sample were 3.92 min and 11.46 min, respectively, which were not significantly different from cement without β-TCP. The degradation time of the BCPC material was longer than that of calcium phosphate cement alone. The healing process was significantly faster for BCPC than for the control and commercial product groups. Therefore, this is the first evidence that BCPC is an attractive option for bone surgery due to its faster stimulation of healing and faster degradation rate.

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Proposal for a New Histological Scoring System for Cartilage Repair

Cited by 6 publications

References 19 publications

Effect of Pore Size on Cell Behavior Using Melt Electrowritten Scaffolds

Effect of Pore Size on Cell Behavior Using Melt Electrowritten Scaffolds

Auricular and laryngeal chondritis in nursery and finishing pigs

Development of injectable chitosan/biphasic calcium phosphate bone cement and in vitro and in vivo evaluation

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