Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?

Rossi, Flávia; Girardello, Raquel; Morais, Carlos L.; Cury, Arlete Emily; Martins, Luciane; Silva, da; Abdala, Edson; Setúbal, João Carlos; Duarte, Alberto José da Silva

doi:10.6061/clinics/2017(10)09

Cited by 37 publications

(33 citation statements)

References 21 publications

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“…coli from this group has been reported on numerous occasions, for example ST10 from human clinical samples in China [ 21 ], ST744 from human and cattle-associated samples in Europe [ 22 , 23 ], ST167 from human infections in Spain and China [ 24 , 25 ], as well as ST48 from hospital sewage and human clinical samples, in China and Switzerland, respectively [ 26 , 27 ]. Additional worldwide clones previously shown to harbor mcr were also confirmed here, and include ST641 [ 28 ], ST410 [ 29 , 30 ], and ST156 [ 31 , 32 ]. Our screening identified two mcr -harboring ST117 E .…”

Section: Resultssupporting

confidence: 78%

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Wise¹,

Estabrook²,

Sahm³

et al. 2018

PLoS ONE

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A set of 908 clinically derived colistin-resistant Enterobacteriaeae isolates collected worldwide in 2014–2016 were screened for the presence of the plasmid-borne mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 genes. In total 3.2% (29/908) of the collection were positive for mcr, including 27 Escherichia coli, 1 Klebsiella pneumoniae and 1 Enterobacter cloacae. Twenty-four isolates possessed genes from the mcr-1 family, including the original mcr-1 (n = 22), as well as mcr-1.2 (n = 1) and mcr-1.5 (n = 1), which each differ from mcr-1 by encoding single amino acid variations. Genes from the mcr-3 family were found in isolates from Thailand, including mcr-3.1 (n = 3) and mcr-3.2 (n = 1). An E. coli isolated from a patient with a urinary tract infection in Colombia contained the recently discovered mcr-5. The full colistin-resistant collection was tested against a panel of antimicrobial agents with ceftazidime-avibactam and tigecycline exhibiting the highest activity.

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Section: Resultssupporting

confidence: 78%

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Wise¹,

Estabrook²,

Sahm³

et al. 2018

PLoS ONE

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“…The ST 405 and ST 156 have been previously reported in association with carbapenem-resistant blaNDM-5 from clinical isolates and chicken meat, respectively [26 27]. In other regions of the world, the ST 405 and ST156 harboring mcr-1 have been reported, such as ST 405 in the United States and Algeria and ST 156 in China and Brazil [28][29][30][31]. ST 405 and ST156…”

Section: Discussionmentioning

confidence: 95%

WITHDRAWN: Discovery of mcr-1 Harboring Incl2 Plasmids from Clinical Isolates of Multiclonal E. Coli prevalent in Pakistan.

Bilal

Rehman

Khan

et al. 2020

Preprint

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Background: Colistin is the last resort antibiotic against multiple drug-resistant (MDR) bacteria found in clinical infections; however, the emergence of plasmid-mediated mcr-1 gene annulled the efficacy of Colistin. This study was planned to determine the prevalence of mcr genes in clinical isolates collected in Pakistan. The molecular types and plasmids of mcr-1 bearing isolates were analysed. Methods: A total of 545 E. coli isolates collected from two major cities of Pakistan were screened for colistin-resistance and mcr genes from June 2018 to September 2019. All positive strains were subjected to antimicrobial susceptibility testing, ESBL, and MBL detection via DDST and CDT. ESBL genes detection, molecular typing, conjugation experiment, plasmid replicon typing, S1 PFGE, and southern hybridization were performed. Results: Four (0.73%) strains of mcr-1 positive isolates were susceptible to meropenem, fosfomycin, and chloramphenicol, including one that showed moderate level resistance to chloramphenicol and fosfomycin. All four strains were ESBL positive and harbored the blaCTX-M-15 gene, while three of the isolates also harbored the blaTEM-1 gene. Molecular typing revealed that four isolates belonged to diverse clonal types, mostly (75%) from avian pathogenic E. coli lineage. The mcr-1 gene was present on ~ 60 kb Incl2 plasmid, which was successfully transconjugated. Conclusion: The mcr-1 gene's detection in diverse clonal types from MDR clinical isolates enforces priority basis large scale surveillance studies followed by corrective actions to prevent the spread of the mcr-1 gene in clinical, poultry, and environmental setting.

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“…There have been many reports of MCR producers in Brazil, with MCR-1 being the only variant systematically identified. These isolates were a single Salmonella enterica serotype Typhimurium isolate that was recovered from retail meat (18) and E. coli isolates recovered from chicken meat (19), from migratory penguins (20), on public beaches (21), or from patients with bloodstream infections (22,23). Also, KPC-2-producing E. coli isolates (24) and KPC-2-producing Klebsiella pneumoniae isolates belonging to ST392 and ST437 (25,26) were identified.…”

Section: Discussionmentioning

confidence: 99%

Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil

Kieffer

Nordmann

Moreno

et al. 2018

Antimicrob Agents Chemother

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A collection of 126 pigs was screened for carriage of colistin-resistant in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1 and MCR-2 as a result of the addition of a phosphoethanolamine group to the lipid A moiety. Genetic analysis showed that the gene was carried by an IncA/C plasmid and was embedded in an original genetic environment. This study reports the occurrence of the MCR-3-like determinant in South America and is the first to demonstrate the functionality of this group of enzymes as a phosphoethanolamine transferase.

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Plasmid-mediated mcr-1 in carbapenem-susceptible Escherichia coli ST156 causing a blood infection: an unnoticeable spread of colistin resistance in Brazil?

Cited by 37 publications

References 21 publications

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program

WITHDRAWN: Discovery of mcr-1 Harboring Incl2 Plasmids from Clinical Isolates of Multiclonal E. Coli prevalent in Pakistan.

Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil

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