The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients

Abstract

OBJECTIVE:Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients.METHODS:Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction.RESULTS:Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p<0.001). Circulating miR-424 levels were reduced 3.6-fold in stable coronary artery disease patients (1.18 ± 0.60) and 5-fold in unstable coronary artery disease patients (0.86 ± 0.54) compared with non-coronary artery disease patients (4.35 ± 2.20) (p<0.001). In contrast, circulating miR-765 levels were elevated 3.98-fold in stable coronary artery disease patients (6.09 ± 2.27) and 5.33-fold in unstable coronary artery disease patients (8.17 ± 2.77) compared with non-coronary artery disease patients (1.53 ± 0.99) (p<0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve for circulating miR-149, miR-424 and miR-765 were 0.938, 0.919 and 0.968 in stable CAD patients and 0.951, 0.960 and 0.977 in unstable coronary artery disease patients compared with non-coronary artery disease patients.CONCLUSION:Our results suggest that circulating miR-149, miR-424 and miR-765 might be novel, non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged patients. However, future prospective trials in large patient cohorts are necessary before reaching a solid conclusion.

Protective Effects of Microrna-22 Against Endothelial Cell Injury by Targeting NLRP3 Through Suppression of the Inflammasome Signaling Pathway in a Rat Model of Coronary Heart Disease

“…Coronary heart disease (CHD) as a common cardiovascular disease that mainly results from the build-up of atherosclerotic plaques, which narrows coronary arteries and decreases blood flow to the heart [1]. Although recent advances in early detection and therapies have been developed, CHD is still the major cause of sudden cardiac death and hospitalization around the world [2, 3].…”

Gastric bypass surgery with exercise alters plasma microRNAs that predict improvements in cardiometabolic risk

“…55 In addition, over a fourfold reduction in the circulating levels of miR-149 was reported in middle-aged and elderly patients with coronary artery disease. 56,57 Furthermore, miR-149 was reported significantly downregulated during cardiac remodeling in response to acute myocardial infarction in mouse and human hearts. 58 Taken together, our results suggest that exercise-induced miR-149 (for example, in skeletal and cardiac muscle tissue) adds onto the beneficial cardiometabolic improvements of bariatric surgery.…”

Circulating microRNAs predict future fatal myocardial infarction in healthy individuals – The HUNT study

“…Interestingly, several circulating miR differed significantly between individuals that subsequently suffered a fatal AMI and those that remained healthy during the 10-year follow-up period. Common features for several of these miRs are their relevance for the different steps of the atherosclerotic process [42][43][44][45][46][47][48].…”