Treatment of osteochondral injuries with platelet gel

Danieli, Marcus Vinicius; Pereira, Hamilton da Rosa; Carneiro, Carlos Augusto de Sá; Felisbino, Sérgio Luiz; Deffune, Elenice

doi:10.6061/clinics/2014(10)08

Cited by 5 publications

(4 citation statements)

References 29 publications

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“… 25 , 34 A recent study reported that the histological evaluation of an osteochondral lesion treated with PRP over 180 days exhibited superior results for the PRP group, compared to the untreated group. 35 These results are in line with the findings of our study, which present good histological results (cell morphology, surface regularity, chondral thickness, and repair tissue integration) for the PRP-treated groups compared to the controls.…”

Section: Discussionsupporting

confidence: 92%

The effectiveness of platelet-rich plasma gel on full-thickness cartilage defect repair in a rabbit model

Slimi

Zribi

Trigui

et al. 2021

Bone & Joint Research

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Aims The present study investigates the effectiveness of platelet-rich plasma (PRP) gel without adjunct to induce cartilage regeneration in large osteochondral defects in a rabbit model. Methods A bilateral osteochondral defect was created in the femoral trochlear groove of 14 New Zealand white rabbits. The right knees were filled with PRP gel and the contralateral knees remained untreated and served as control sides. Some animals were killed at week 3 and others at week 12 postoperatively. The joints were harvested and assessed by Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) MRI scoring system, and examined using the International Cartilage Repair Society (ICRS) macroscopic and ICRS histological scoring systems. Additionally, the collagen type II content was evaluated by the immunohistochemical staining. Results After 12 weeks post-surgery, the defects of the PRP group were repaired by hyaline cartilage-like tissue. However, incomplete cartilage regeneration was observed in the PRP group for three weeks. The control groups showed fibrocartilaginous or fibrous tissue, respectively, at each timepoint. Conclusion Our study proved that the use of PRP gel without any adjuncts could successfully produce a good healing response and resurface the osteochondral defect with a better quality of cartilage in a rabbit model. Cite this article: Bone Joint Res 2021;10(3):192–202.

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Section: Discussionsupporting

confidence: 92%

The effectiveness of platelet-rich plasma gel on full-thickness cartilage defect repair in a rabbit model

Slimi

Zribi

Trigui

et al. 2021

Bone & Joint Research

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“…Milano et al [24], Hapa et al [27] and Huh et al [13] compared the combined application of PRP and microfracture technique with the microfracture technique alone and found that applying PRP increases the quality of the newly formed cartilage. Carneiro et al [6], Danieli et al [7] and Kinoshita et al [15] reported that PRFM has reparative properties and stimulates fibrocartilaginous tissue formation. Bahmanpour et al [5] compared the PRFM and PRP for the treatment of hyaline cartilage defects on rabbit knee and concluded that PRFM is superior to PRP treatment, aligning with the presented findings.…”

Section: Dıscussıonmentioning

confidence: 99%

“…PRFM is prepared from the patient’s autologous blood that has high concentration of fibrin and growth factors [31]. Unlike PRP, PRFM needs any additional activation steps and can be applied simultaneously with the surgery [6, 7, 15].…”

Section: Introductıonmentioning

confidence: 99%

Applying additional autologous platelet‐rich fibrin matrix or serial platelet‐rich plasma to microfracture technique increases the quality of the repaired cartilage

Balta,

Kurnaz

2023

Knee surg. sports traumatol. arthrosc.

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PurposeThe aim of the present study is to investigate and compare the effects of biological adjuvants (platelet-rich plasma, platelet-rich fibrin matrix) and microfracture technique individually and in combination on full thickness chondral defects in a rabbit model. Methods A total of 60 New Zealand White rabbits were randomly divided into six groups according to treatment modality as follows: control (C), microfracture (MF), platelet-rich plasma (PRP), platelet-rich fibrin matrix (PRFM), platelet-rich fibrin matrix after microfracture (MF + PRFM) and platelet-rich plasma after microfracture (MF + PRP) groups. The cartilage repair tissue was assessed histologically via International Cartilage Repair Score (ICRS) and macroscopically via ICRS macroscopic assessment scale. Results It was shown that overall macroscopic scores of the groups with MF were higher than those of the groups without MF. The cell morphology observed in the defect areas was mostly characterized with non-chondrocyte cells in the groups without MF, whereas chondrocyte cells mostly prevailed in the groups with MF. There was a greater integration through the cartilage-like tissue in the MF + PRP and MF + PRFM groups. The control group showed either fissures or fissures partially filled with fibrous tissue. When the groups were individually examined, there were statistically significant differences between the control and MF groups (p = 0.002), between the control and MF + PRFM groups (p = 0.001), between the control and MF + PRP groups (p < 0.001), between the PRFM and MF + PRFM groups (p = 0.014) and between the PRFM and MF + PRP (p = 0.023) groups in terms of histological evaluation scores. ConclusionThe application of PRP and PRFM in combination with MF treatment exhibited a positive impact on the repair and restoration of cartilage, and produced better outcomes than the individual use of PRP and PRFM. Nevertheless, in the treatment of full thickness chondral defects, the use of PRFM injection is recommended, which is performed intraoperatively at a single time and with no difficulty of repeating after surgery, instead of serial PRP injections based on the macroscopic and histological results obtained in the present study indicating that there was no significant difference between the use of these two adjuvants. KeywordsPlatelet-rich plasma • Platelet-rich fibrin matrix • Full thickness chondral defect • Microfracture • Rabbit model • İn vivo • Cartilage • Knee * Orhan Balta

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“…morphologic changes including cell shrinkage, condensation and chromatin condensation and nucleosomal fragmentation [14]. The sections stained with safranin O were graded as; 0: normal, 1: slightly reduced, 2: moderately reduced, 3: none (or almost none) and the sections stained with toluidine blue were graded as; 0: normal, 1: slightly reduced, 2: markedly reduced, 3: no metachromatic change according to the histological grading scale described by previous reports [15][16][17].…”

Section: Plos Onementioning

confidence: 99%

Histological and mechanical comparisons of arytenoid cartilage between 4 brachycephalic and 8 non-brachycephalic dogs: A pilot study

Tokunaga

Ehrhart²,

Monnet

2020

PLoS ONE

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Brachycephalic airway syndrome (BAS) is a well-established cause of respiratory distress in dogs. BAS without surgical correction results in eventual laryngeal collapse. Arytenoid lateralization has been used to treat severe laryngeal collapse with some highly variable results. Chondromalacia and decreased stiffness of the arytenoid cartilage has been postulated a source of failure after arytenoid lateralization but no report of the histological characteristics and mechanical strength of arytenoid cartilage in brachycephalic dogs has been reported. Here we report histological and mechanical features in arytenoid cartilage of brachycephalic dogs. We identified the arytenoid cartilage in brachycephalic dogs presented degenerative histological characteristics and decreased load to failure and stiffness compared to that in non-brachycephalic dogs. Together, these observations suggest that degenerative condition of arytenoid cartilage in brachycephalic dogs could contribute to chondromalacia and mechanical weakness of arytenoid cartilage and result in cause of failure after arytenoid lateralization.

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Treatment of osteochondral injuries with platelet gel

Cited by 5 publications

References 29 publications

The effectiveness of platelet-rich plasma gel on full-thickness cartilage defect repair in a rabbit model

The effectiveness of platelet-rich plasma gel on full-thickness cartilage defect repair in a rabbit model

Applying additional autologous platelet‐rich fibrin matrix or serial platelet‐rich plasma to microfracture technique increases the quality of the repaired cartilage

Histological and mechanical comparisons of arytenoid cartilage between 4 brachycephalic and 8 non-brachycephalic dogs: A pilot study

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