The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes

Wahba

Zagazig Journal of Pharmaceutical Sciences

et al. 2016

Diabetic nephropathy (DN) is the main cause of end stage renal disease. Complex mechanisms are implicated in the pathogenesis of DN including the accumulation of advanced glycation end products (AGEs), oxidative stress, and inflammation mediated through the upregulation of toll-like receptor 4 (TLR4). Hibiscus sabdariffa is widely used in food products possessing anti-oxidative, anti-inflammatory, anti-diabetic, and anti-hypertensive effects. The present study aimed to investigate the role of hibiscus extract against streptozotocin(STZ)induced DN compared to lisinopril, and to evaluate the effects of their combined administration. Rats were treated with hibiscus extract (100, 200, or 400 mg/kg), lisinopril (5 mg/kg), or lisinopril plus hibiscus extract (2.5/100 mg/kg) for 8 weeks. Hibiscus extract caused a dosedependent improvement of nephropathy, and suppressed oxidative stress, inflammation, and the upregulation of TLR4 induced by STZ. The effects of hibiscus extract (400 mg/kg) were comparable to lisinopril (5 mg/kg). Interestingly, the combined administration of lisinopril plus hibiscus extract (2.5/100 mg/kg) significantly improved the manifestations of nephropathy better than lisinopril or hibiscus extract alone. These results might support the application of hibiscus extract as an alternative to the conventional angiotensin-converting enzyme inhibitors (ACEIs) or at least as an adjunctive to augment their effects in DN.

Section: Discussionsupporting

confidence: 93%

Hibiscus sabdariffa extract augments the renoprotective effect of lisinopril against streptozotocin-induced diabetic nephropathy in rats

Wahba

Zagazig Journal of Pharmaceutical Sciences

et al. 2016

“…According to Waseem et al (2017), protein carbonylation indicates oxidative protein damage resulting from cisplatin-induced oxidative stress. PmEO100CP and BCP pre-treated rats significantly restored protein carbonyl content in the liver similar to the role of natural product on protein carbonylation previously shown by Mohamed et al (2013). 8-OHdG is the most significant indicator of DNA damage (Koyuncu et al 2017).…”

Section: Discussionsupporting

confidence: 78%

Preventive Effects of Polygonum minus Essential Oil on Cisplatin-Induced Hepatotoxicity in Sprague Dawley Rats

Rashid

Hussan

Hamid³

et al. 2019

JSM

Self Cite

Cisplatin is a chemotherapeutic agent widely used in treating various types of cancer. However, its usage is restricted due to the adverse hepatoxicity, as seen in approximately 36% of cancer patients receiving cisplatin treatment. Polygonum minus essential oil has high antioxidant capacity, and is enriched with terpenoids and phenolic compounds. The objective of this study was to investigate effects of P. minus essential oil (PmEO) supplementation on cisplatininduced hepatotoxicity in rats. Male rats were divided into seven different groups, namely: control (C), cisplatininduced (CP), positive control with β-caryophyllene 150 mg/kg (BCP), PmEO 100 mg/kg (PmEO100CP), PmEO 200 mg/kg (PmEO200CP), PmEO 400 mg/kg (PmEO400CP) and PmEO 400 mg/kg alone (PmEO400). PmEO and BCP was given orally for 14 days prior to a single dose cisplatin (10 mg/kg) injection on day 15 and rats were sacrificed on day 18. Liver enzymes, histology, ultrastructural morphology and oxidative stress markers such as glutathione, glutathione peroxidase, catalase, superoxide dismutase and malondialdehyde were assayed. Compared to controls, levels of transaminase enzymes, serum bilirubin and oxidative stress were all increased in CP, PmEO200CP and PmEO400CP groups. However, only PmEO100CP and BCP groups reduced these increases in level of transaminase enzymes and oxidative stress compared to CP group. On both light microscopic and ultrastructural examination, CP and PmEO400CP groups showed hepatotoxicity, exhibited by cytoplasmic vacuolation, congested blood sinusoids and increased number of Kupffer cells. However, these changes were minimized in the PmEO100CP group. Therefore, we concluded that PmEO given at 100 mg/kg has preventive effect against cisplatin-induced hepatotoxicity in rats. ABSTRAKCisplatin adalah agen kemoterapi yang digunakan secara meluas untuk merawat pelbagai jenis kanser. Walau bagaimanapun, penggunaannya adalah sangat terhad disebabkan oleh berlakunya kehepatoksikan dalam kalangan kira-kira 36% pesakit kanser yang menerima rawatan cisplatin. Minyak pati Polygonum minus mempunyai keupayaan antioksidan yang tinggi dan diperkaya dengan terpenoid dan sebatian fenolik. Objektif kajian ini adalah untuk mengkaji kesan suplemen minyak pati P. minus (PmEO) ke atas cisplatin-aruhan kehepatoksikan di dalam tikus. Tikus jantan dibahagikan kepada tujuh kumpulan yang berbeza iaitu: kawalan (C), cisplatin-aruhan (CP), kawalan positif dengan β-caryophyllene 150 mg/kg (BCP), PmEO 100 mg/kg (PmEO100CP), PmEO 200 mg/kg (PmEO200CP), PmEO 400 mg/kg (PmEO400CP) dan PmEO 400 mg/kg sahaja (PmEO400). PmEO dan BCP diberikan secara oral selama 14 hari sebelum dos tunggal cisplatin (10 mg/kg) disuntik pada hari ke 15 dan tikus dikorbankan pada hari ke 18. Enzim hati, histologi, morfologi ultrastruktur dan penunjuk tekanan oksidatif seperti glutation, glutation peroksida, katalase, superoksida dismutase dan malondialdehid diasai. Berbanding dengan kawalan, tahap enzim transaminase, serum bilirubin dan tekanan oksidatif semuanya meningkat dalam kumpulan ...

“…Hence continuously decreased serum antioxidant status or continuously increase oxidative status can catastrophically damage to the epithelial lining of lungs and allowing the movements of HP in the blood which can jeopardize the wellbeing of erythrocyte. In other study it was well demonstrated that RBC membrane of rats can't sustain the oxidative stress of streptozotocin-induced diabetes, however in the same study it was demonstrated that protective agent (antioxidants) when administer in adequate dose enables the RBC to sustain the oxidative stress of diabetes [18][19][20]. Cigarettes smoking has reached to alarming situation in all societies, irrespective of the gender and age.…”

Section: Discussionmentioning

confidence: 99%

Perpetual Smoking Compromise the Osmotic Fragility of RBC

Mushtaq¹

2017

Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.