Assessing the benefits of rosiglitazone in women with polycystic ovary syndrome through its effects on insulin-like growth factor 1, insulin-like growth factor-binding protein-3 and insulin resistance: a pilot study

Batista, José Gomes; Soares-Jr, José Maria; Maganhin, Carla Cristina; Simões, Ricardo Santos; Tomaz, Geraldez; Baracat, Edmund Chada

doi:10.6061/clinics/2012(03)14

Cited by 20 publications

(24 citation statements)

References 28 publications

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“…23 Moreover, the levels of IGF1 are elevated and may affect ovarian function and increase androgen production in PCOS. 24 Herein, we identified the expression of HOTAIR, miR-130a and IGF1 in the ovarian tissues and granulosa cells of PCOS rat models and verified the regulatory relationships among them, so as to determine the mechanisms of controlling the endocrine disorders and activities of ovarian granulosa cells.…”

Section: Discussionmentioning

confidence: 90%

Down‐regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin‐like growth factor 1 via microRNA‐130a

Jiang

Xue

et al. 2019

J Cellular Molecular Medi

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It has been found that long noncoding RNA HOTAIR, microRNA‐130a (miR‐130a) and insulin‐like growth factor 1 (IGF1) expression are associated with ovarian cancer, thus, we hypothesised that the HOTAIR/miR‐130a/IGF1 axis might associate with endocrine disorders and biological behaviours of ovarian granulosa cells in rat models of polycystic ovary syndrome (PCOS). PCOS rat models were established by injection of dehydro‐isoandrosterone, followed by treatment of si‐HOTAIR, oe‐HOTAIR, miR‐130a mimics or miR‐130a inhibitors. Serum hormonal levels were determined to evaluate endocrine conditions. The effect of HOTAIR and miR‐130a on activities of isolated ovarian granulosa cells was assessed, as well as the involvement of IGF1.In the ovarian tissues and granulosa cells of PCOS rat models, highly expressed HOTAIR and IGF1 and poorly expressed miR‐130a were identified. In response to oe‐HOTAIR, serum levels of E2, T and LH were increased and serum levels of FSH were reduced; the proliferation of granulosa cells was reduced and apoptosis was promoted; notably, expression of miR‐130a was reduced while expression of IGF1 was increased. The treatment of si‐HOTAIR reversed the situation. Furthermore, the binding of HOTAIR to miR‐130a and targeting relationship of miR‐130a and IGF1 were confirmed. LncRNA HOTAIR up‐regulates the expression of IGF1 and aggravates the endocrine disorders and granulosa cell apoptosis through competitive binding to miR‐130a in rat models of PCOS. Based on our finding, we predict that competitive binding of HOTAIR to miR‐130a may act as a novel target for the molecular treatment of PCOS.

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Section: Discussionmentioning

confidence: 90%

Down‐regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin‐like growth factor 1 via microRNA‐130a

Jiang

Xue

et al. 2019

J Cellular Molecular Medi

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“…dIScuSSIon Polycystic ovary syndrome has been considered as one of the most controversial entities in gynecological endocrinology, as it includes a wide spectrum of clinical signs and symptoms. 1 The heterogeneity of this syndrome has led to the creation of numerous discussions and varying consensuses for diagnosis. However, its pathophysiology is not yet fully understood.…”

Section: Resultsmentioning

confidence: 99%

“…1 According to the Rotterdam consensus in 2003, the diagnostic criteria for PCOS include the presence of hyperandrogenism, ovarian cysts on ultrasound and oligoovulation and anovulation. 2 There is evidence that some genes are responsible for the etiology of this syndrome, including those which codify the enzymes related to androgenic metabolism in the ovary.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical evaluation of proliferation, apoptosis and steroidogenic enzymes in the ovary of rats with polycystic ovary

Lombardi

Simões

Maganhin

et al. 2014

Rev. Assoc. Med. Bras.

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ImmunohIstochemIcal evaluatIon of prolIferatIon, apoptosIs and steroIdogenIc enzymes In the ovary of rats wIth polycystIc ovary rev assoc med Bras 2014; 60(4):349-356 349original articleImmunohistochemical evaluation of proliferation, apoptosis and steroidogenic enzymes in the ovary of rats with polycystic ovary Conflict of interest: noneObjective: to evaluate the immunohistochemical expression of proliferative, apoptotic and steroidogenic enzyme markers in the ovaries of rats with polycystic ovary syndrome (PCOS). Methods: twenty rats were divided into two groups: GCtrl -estrous phase, and PCOS -with polycystic ovaries. The GCtrl animals were subjected to a lighting period from 7 am to 7 pm, while the animals with PCOS group remained with continuous lighting for 60 days. Subsequently, the animals were anesthetized, the ovaries were removed and fixed in 10% formaldehyde, prior to paraffin embedding. Sections were stained using H.E. or subjected to immunohistochemical methods for the detection of Ki-67, cleaved caspase-3, CYP11A1, CYP17A1 and CYP19A1. The results were analyzed using Student's t-test (p < 0,05). Results: morphological results showed evidence of interstitial cells originating from the inner theca cells of degenerating ovarian cysts in PCOS. Immunoexpression of Ki-67 was higher in the granulosa cells in GCtrl, and the theca interna cells in PCOS, while cleaved caspase-3 was higher in granulosa cells of ovarian cysts from PCOS and in the theca interna cells of GCtrl. Immunoreactivity of CYP11A1 in the theca interna, granulosa and interstitial cells was similar between the two groups, while CYP17A1 and CYP19A1 were higher in the granulosa and interstitial cells in the PCOS group. Conclusion: the results indicate that the interstitial cells are derived from the theca interna and that enzymatic changes occur in the theca interna and interstitial cells in ovaries of rats with PCOS, responsible for the high levels of androgens and estradiol.

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“…Entre as inúmeras causas de infertilidade, destaca-se, por sua frequência, a síndrome dos ovários policísticos (SOP) que atinge aproximadamente 5 a 10% das mulheres durante a vida reprodutiva 1,2 . O hiperandrogenismo é uma das características mais notáveis dessa síndrome, que resulta em acne, hirsutismo e disfunção ovulatória 2,3 .…”

Section: Introductionunclassified

Morfologia das células intersticiais de ovários policísticos de ratas: um estudo experimental

Lombardi

Simões

Maganhin

et al. 2012

Rev. Bras. Ginecol. Obstet.

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Assessing the benefits of rosiglitazone in women with polycystic ovary syndrome through its effects on insulin-like growth factor 1, insulin-like growth factor-binding protein-3 and insulin resistance: a pilot study

Cited by 20 publications

References 28 publications

Down‐regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin‐like growth factor 1 via microRNA‐130a

Down‐regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin‐like growth factor 1 via microRNA‐130a

Immunohistochemical evaluation of proliferation, apoptosis and steroidogenic enzymes in the ovary of rats with polycystic ovary

Morfologia das células intersticiais de ovários policísticos de ratas: um estudo experimental

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