Storage of pollen and properties of olive stigma for breeding purposes

Zambon, Carolina Ruiz; Silva, Luiz Fernando de Oliveira da; Pio, Rafael; Bianchini, Flávio Gabriel; Oliveira, Adelson Francisco de

doi:10.5935/1806-6690.20180033

Cited by 9 publications

(9 citation statements)

References 2 publications

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“…In a study conducted with the Manzanillo olive cultivar, the germination of pollens stored at -20 °C decreased by 40% after 365 days (Pinney and Polito, 1990). Besides that, it has been reported that the pollens of the Arbequina olive cultivar can be stored for 60 days at -10 °C (Zambon et al, 2018). The interaction of cultivar × storage time × storage temperature was important for the effect of the storage of olive pollens at different temperatures and storage times on pollen diameters (P <= 0.001) (Table 3).…”

Section: Resultsmentioning

confidence: 87%

Storage and In-vitro Germination of Some Olive Pollens

ŞENBAŞ>¹,

Horzum

Gencer

et al. 2022

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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In this study, the effect of storage at different times and temperatures on in vitro olive pollen germination was investigated. Pollens of Gemlik and Domat cultivars and GE119 and GK138 genotypes were stored at +4 °C, -20 °C, and -80 °C for 7, 35, 200, and 365 days, respectively. In vitro germination status of pollen was determined by the petri agar method, by choosing the most suitable nutrient medium for each olive. The most suitable germination media for Gemlik, Domat, GE119, and GK138 pollens were 50 ml water + 15% sucrose + 0.7% agar + 75 ppm boric acid, 50 ml water + 15% sucrose + 0.7% agar, 50 ml water + 25% sucrose + 0.5% agar, 50 ml water respectively. The interaction effect between storage time, temperatures, and cultivar on pollen germination and diameter was determined. At the end of the storage period, the highest pollen germination and diameter were observed in the Gemlik cultivar. Additionally, -80 °C temperature for Gemlik and Domat cultivars and -20 °C temperature for GE119 and GK138 genotypes were suitable for 35 days of storage. All olive pollens in the current study had germination rates below 9% in the following storage periods. The results show that storing olive pollens at sub-zero temperatures will reduce the need for daily fresh pollen collection required for important scientific studies such as breeding and artificial pollination.

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Section: Resultsmentioning

confidence: 87%

Storage and In-vitro Germination of Some Olive Pollens

ŞENBAŞ>¹,

Horzum

Gencer

et al. 2022

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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“…Low temperature storage of pollen grains is an important strategy and help breeders to develop new cultivars through hybridization, especially in those species in which the durability of the flowers is reduced and the synchronization of flowering is difficult (Giovannini et al, 2017;Zambon et al, 2018). This is the case of the cultivars of amaryllis evaluated herein, in which the durability of the flowers is relatively short, around 5-10 days from anthesis to the beginning of flower senescence, as well as the beginning of the development of the inflorescence is affected by different factors, such as the presence of diseases in the bulb, the size of the bulb and the conditions and time of storage of pollen grains.…”

Section: Effects Of the Interaction Between The Cultivar And The Stormentioning

confidence: 99%

“…Other simplified methods also use low-temperatures for preservation of pollen grains, but are applied for short periods of storage (days, weeks, months) including freezer storage (-10 to -20 °C) and refrigerator (5-10 ºC), already reported in Citrus species (Ali & Perveen, 2014), castor (Vargas et al, 2009), olives (Zambon et al, 2018), roses (Giovannini et al, 2015) and kiwi (Borghezan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…It is important to emphasize that in breeding programs, one objective is the hand cross pollination of species with different bloom season, in which these cheaper techniques of freezer or refrigerator storage meet the demands of the breeders (Zambon et al, 2018), since in this case both parents have at least one flowering throughout the year.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.)

2019

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The determination of viability and the preservation of pollen grains are useful tools for breeding of ornamentals, especially when the synchronization of flowering between species or cultivars does not occur naturally. Thus, the objective of this study was to characterize the flowering and to evaluate the preservation and maintenance of the viability of pollen grains in cultivars of amaryllis (Hippeastrum sp.) subjected to three storage temperatures. Anthers of five cultivars of amaryllis were collected from 12 to 24 hours after anthesis. One part of the anthers was immediately used for germination tests using in vitro germination in culture medium and the other part was stored at temperatures of 25, 8 and-20 °C. Pollen grains were germinated in culture medium at 0, 20, 75, 125, 165, 205 and 245 days after storage. All cultivars of amaryllis showed an accelerated loss of viability at 25 °C storage temperature. The 'Bull' cultivar showed higher recalcitrance to cold-storage, while 'Intokazie' and 'Pink Panther' cultivars showed higher longevity and higher percentage of germination along storage. The temperature of-20 °C resulted in the best responses for the maintenance of the integrity and preservation of the viability of pollen grains of different cultivars of amaryllis.

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“…Stigma receptivity was chemically evaluated with 3% hydrogen peroxide (H 2 O 2 ). This evaluation involved depositing H 2 O 2 in the stigma of flowers by considering receptive stigmas that rapidly formed bubbles (Zambon et al, 2018). Evaluation of stigma receptivity was performed at pre-anthesis and anthesis stages using a periodicity schedule, which started at 7:00 am and continued until 2:00 pm.…”

Section: Stigma Receptivitymentioning

confidence: 99%

Rubus glaucus Benth.: morphology and floral biology aimed at plant breeding processes

Gutiérrez

Marulanda

López

et al. 2019

Rev. Fac. Nac. Agron. Medellín

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Rubus glaucus is widely distributed throughout the three mountain ranges of Colombia, where the blackberry growers have highlighted the need to standardize the supply of planting material, starting with plant breeding schemes that lead to more productive varieties with morphological characteristics that ease agricultural activities. Plant breeding activities have improved by considering the pollination mechanisms of plants. The implementation of controlled sexual hybridization depends on these pollination mechanisms, and several plant breeding methods have been adapted to crop pollination patterns. Morphological characteristics and studies on the floral biology of R. glaucus Benth were conducted to improve plant breeding processes. In addition, a study on pollen viability and stigma receptivity were performed. The reported morphological characteristics of R. glaucus enabled characterization of its flowers as complete and perfect with a regular, actinomorphic, perianth heterochlamydeous, dialipetalous, and dialisepalous structure. Meanwhile, the evaluation of different collection times for pollen viability revealed significant differences. The highest pollen viability occurred at 10:00 am, followed by 9:00 am. Qualitative evaluation of stigma receptivity led to the conclusion that the highest stigma receptivity is at anthesis at 12:00 m.

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Storage of pollen and properties of olive stigma for breeding purposes

Cited by 9 publications

References 2 publications

Storage and In-vitro Germination of Some Olive Pollens

Storage and In-vitro Germination of Some Olive Pollens

Characterization of cultivars and low-temperature pollen grain storage in amaryllis (Hippeastrum sp.)

Rubus glaucus Benth.: morphology and floral biology aimed at plant breeding processes

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