Study of Novel Coating Strategy: Simutaneous Coating of VEGF and anti-CD34 Antibody

Song, Chunli; Li, Qian; Yu, Yunpeng; Wang, Guan; Wang, Jinpeng; Lü, Yang; Zhang, Jichang; Diao, Hong-Ying; Liu, Jian‐Gen; Liu, Yihang; Liu, Jia; Liu, Ying; Cai, Dan; Liu, Bin

doi:10.5935/1678-9741.20150016

Cited by 5 publications

(5 citation statements)

References 23 publications

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“…The most popular preparation methods for stent coating include dipping 52 and spraying 53,54 , but such methods are unwieldy considering the hydrophobicity and hydrophilicity of the drugs. Hossfeld et al 55 prepared a bioactive coronary stent coating based on layer-by-layer technology for siRNA release, whereas Song et al 56 demonstrated multi-step immersion for the simultaneous coating of VEGF and anti-CD34 antibody. In this study, we sought to fabricate a novel hydrophobic core/hydrophilic shell particle coating on the surface of the implant using coaxial electrospraying.…”

Section: Discussionmentioning

confidence: 99%

Design and testing of hydrophobic core/hydrophilic shell nano/micro particles for drug-eluting stent coating

Wang

Huang

et al. 2018

NPG Asia Mater

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In this study, we designed a novel drug-eluting coating for vascular implants consisting of a core coating of the antiproliferative drug docetaxel (DTX) and a shell coating of the platelet glycoprotein IIb/IIIa receptor monoclonal antibody SZ-21. The core/shell structure was sprayed onto the surface of 316L stainless steel stents using a coaxial electrospray process with the aim of creating a coating that exhibited a differential release of the two drugs. The prepared stents displayed a uniform coating consisting of nano/micro particles. In vitro drug release experiments were performed, and we demonstrated that a biphasic mathematical model was capable of capturing the data, indicating that the release of the two drugs conformed to a diffusion-controlled release system. We demonstrated that our coating was capable of inhibiting the adhesion and activation of platelets, as well as the proliferation and migration of smooth muscle cells (SMCs), indicating its good biocompatibility and anti-proliferation qualities. In an in vivo porcine coronary artery model, the SZ-21/DTX drug-loaded hydrophobic core/hydrophilic shell particle coating stents were observed to promote re-endothelialization and inhibit neointimal hyperplasia. This core/shell particle-coated stent may serve as part of a new strategy for the differential release of different functional drugs to sequentially target thrombosis and in-stent restenosis during the vascular repair process and ensure rapid re-endothelialization in the field of cardiovascular disease.

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Section: Discussionmentioning

confidence: 99%

Design and testing of hydrophobic core/hydrophilic shell nano/micro particles for drug-eluting stent coating

Wang

Huang

et al. 2018

NPG Asia Mater

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“…CCL18 is produced by monocytes and plays an important role, in part, by activating the immune system and by inducing tolerance and homeostasis under steady-state conditions. In addition, CCL18 promotes the maturation of macrophages to phenotype M2, which in turn promotes immunosuppression and healing [30,32]. Higher expression levels of CCL18 were observed in the LSR samples at the last time point we determined (Figure 3C).…”

Section: Discussionmentioning

confidence: 80%

“…A confluent endothelial cell layer on material surfaces is widely considered an approach to improve the biocompatibility of implanted cardiovascular materials. Therefore, VEGF coatings or functionalization have been used to protect stents from in-stent restenosis, induce angiogenesis, or protect small diameter vascular grafts from thrombosis or degeneration by inducing endothelial formation [32][33][34]. However, VEGF can also lead to increased neo-intimal hyperplasia [34], and there are implants where integration with the surrounding tissue should be avoided, such as transvenous pace maker electrodes.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes

et al. 2021

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The biocompatibility of medical devices, such as implants and prostheses, is strongly determined by the host’s immune response to the implanted material. Monocytes and macrophages are main actors of the so-called foreign body reaction. The innate immune system macrophages (M) can be broadly classified into the pro-inflammatory M1-type and the anti-inflammatory, pro-healing M2-type. While a transient inflammatory initial state can be helpful during an infection, persistent inflammation interferes with proper healing and subsequent regeneration. The functional orientation of the immune response, mirrored by monocyte polarization, during interaction with different biomaterials has not yet been sufficiently explored. In implant manufacturing, thermoplastic polyurethane (TPU) represents the state-of-the-art material. The constantly growing areas of application and the associated necessary adaptations make the optimization of these materials indispensable. In the present study, modified liquid silicone rubber (LSR) were compared with two of the most commonly used TPUs, in terms of monocyte adhesion and M1/M2 polarization in vitro. Human monocytes isolated from venous blood were evaluated for their ability to adhere to various biomaterials, their gene expression profile, and their cytokine release. Based on the results, the different polymers exhibit different potential to bias monocytes with respect to early pro-inflammatory cytokine production and gene transcription. Furthermore, none of our test materials showed a clear trend towards M1 or M2 polarization. However, we were able to evaluate the inflammatory potential of the materials, with the classic TPUs appearing to be the most unreactive compared to the silicone-based materials.

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“…Fixation of CD34 antibody on the surface of biological materials can promote the adhesion of EPCs and the formation of endothelium. 171 CD34 monoclonal antibody was introduced into the material surface by EDC/NHS cross-linking with the surface-encapsulated ECM. 172 Anti-CD34 antibody stimulated the adhesion and proliferation of EPCs on the material surface, and improved the endothelialization ability and long-term patency rate of graft.Anti-CD34 antibodies can also be covalently grafted onto a hydroxyl-terminated e ePTFE surface with a lubricant injection layer by silanization, and the introduction of antibody enhanced the ability of the material surface to capture ECs specifically.…”

Section: Surface Design Based On Biological Function Intervention And...mentioning

confidence: 99%

Endothelialization strategy of implant materials surface: The newest research in recent 5 years

Bian

Chen

Weng

et al. 2022

Journal of Applied Biomaterials & Functional Materials

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In recent years, more and more metal or non-metal materials have been used in the treatment of cardiovascular diseases, but the vascular complications after transplantation are still the main factors restricting the clinical application of most grafts, such as acute thrombosis and graft restenosis. Implant materials have been extensively designed and surface optimized by researchers, but it is still too difficult to avoid complications. Natural vascular endodermis has excellent function, anti-coagulant and anti-intimal hyperplasia, and it is also the key to maintaining the homeostasis of normal vascular microenvironment. Therefore, how to promote the adhesion of endothelial cells (ECs) on the surface of cardiovascular materials to achieve endothelialization of the surface is the key to overcoming the complications after implant materialization. At present, the surface endothelialization design of materials based on materials surface science, bioactive molecules, and biological function intervention and feedback has attracted much attention. In this review, we summarize the related research on the surface modification of materials by endothelialization in recent years, and analyze the advantages and challenges of current endothelialization design ideas, explain the relationship between materials, cells, and vascular remodeling in order to find a more ideal endothelialization surface modification strategy for future researchers to meet the requirements of clinical biocompatibility of cardiovascular materials.

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Study of Novel Coating Strategy: Simutaneous Coating of VEGF and anti-CD34 Antibody

Cited by 5 publications

References 23 publications

Design and testing of hydrophobic core/hydrophilic shell nano/micro particles for drug-eluting stent coating

Design and testing of hydrophobic core/hydrophilic shell nano/micro particles for drug-eluting stent coating

In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes

Endothelialization strategy of implant materials surface: The newest research in recent 5 years

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