Replacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation

Abstract

Substituição do tronco da artéria pulmonar em carneiros utilizando heteroenxerto tubular valvado com preservação não-aldeídicaReplacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation 8 Abstract Introduction: The cardiac bioprostheses are related to thromboembolic events, infectious and degenerative diseases. Wear is mainly attributed to the denaturation of collagen. Glutaraldehyde, the predominant method of preservation of bioprostheses, favors the calcification process and limits their durability. Several techniques try to contain the degenerative process of bioprostheses.Objectives: To evaluate the process of calcification in vivo pulmonary valve heterografts preserved in non-aldehydic (L-Hydro®).Methods: Seventeen sheep underwent replacement of the pulmonary artery valved tubular grafts of bovine pericardium. The animals were divided into two groups: Group L-Hydro® (test / n = 14) and Group Glutaraldehyde (control / n = 3). About 150 days after implantation the animals were sacrificed, necropsied and implants subjected to a pathological study, radiological evaluation and measurement of calcium by atomic absorption spectrophotometry. Statistical analysis was obtained through the Fisher's exact test, Student's t or Mann-Whitney test (significance: 5%).Results: The radiological evaluation, the macroscopic and microscopic measurement of serum calcium by atomic absorption spectrophotometry showed increased calcification of the prosthetic group Glutaraldehyde, when compared to 420 Souza HJB, et al. -Replacement of pulmonary artery trunk in sheep using tubular valved heterograft in non-aldehydic preservation Rev Bras Cir Cardiovasc 2012;27(3):419-28denture-HydroR Group L (P = 0.001). Seven animals in Group L-Hydro® (50%) had adherence of the leaflets to the wall of the tube (P = 0.228).Conclusions: Prostheses preserved in L-Hydro® were more resistant to calcification when compared with glutaraldehyde preserved.Descriptors: Bioprosthesis. Glutaral. Polyethylene glycols. Heart valve prosthesis. Heart valve diseases/surgery.

Challenges in Perioperative Animal Care for Orthotopic Implantation of Tissue-Engineered Pulmonary Valves in the Ovine Model

“…Given the similarities in the anatomical and hemodynamic parameters between ovine and human races, sheep are one of the most widely used large animal models in numerous investigations assessing the degeneration and calcification of bioprosthetic valves [3,10,11]. Sheep have a relatively rapid growth rate, allowing the evaluation of valvular implants in terms of growth and remodeling in relation to the recipient's heart growth [12].…”

Performance of xenogeneic pulmonary visceral pleura as bioprosthetic heart valve cusps in swine

“…Cuspal calcification and degeneration, however, are major risks in pulmonary valve replacement (especially in younger patients) ( 11 – 13 ). Calcification of bioprosthetic heart valves in recipient patients causes deterioration of valvular function and eventually requires reoperation.…”

Estudo piloto comparando artéria mesentérica bovina e enxertos de politetrafluoroetileno expandido como acessos de hemodiálise não autógenos

“…Graft healing is completed by incorporation of myofibroblasts into the collagen and elastin framework in the tunica media of the bovine mesenteric artery. 4,5 The objective of this study was to compare the performance of AVFs constructed using ePTE vascular prostheses with AVFs constructed using vascular bioprostheses preserved with L-Hydro technology, according to the following parameters: primary patency, assisted primary patency and secondary patency; surgical handling properties of the vascular prostheses used to construct AVFs; and complications related to the vascular prostheses used to construct AVFs.…”