Molecular association of pathogenicity and resistance to multiple antimicrobials in
            Acinetobacter baumannii strains recovered from patients with diverse infectious
            diseases

França, Rafaela Oliveira; Costa, Priscila Simão; Milanez, Guilherme Luiz; Bomfim, Maria Rosa Quaresma; Gonçalves, Ricardo; Farias, Luiz M.; Nobre, Vandack; Santos, Simone Gonçalves dos

doi:10.5935/1676-2444.20180049

Cited by 4 publications

(9 citation statements)

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“…The presence of this microorganism -especially the one containing the blaOXA23 gene -in the community represents significant health risk because its dissemination increase community and nosocomial infections, contributing to higher rates of antimicrobial resistance. Reports on A. baumannii strains carrying the blaOXA23 gene resistant to carbapenems are especially found in isolates from biological samples of ICU patients [19][20][21]. A study conducted in five Brazilian states showed a 94.2% prevalence of the carbapenemresistant blaOXA23 gene [22], which is very similar to data here reported (i.e., found in all cases).…”

Section: Discussionsupporting

confidence: 89%

Associated factors of Acinetobacter baumannii complex in hospitalized patients: A case-control study

Vandresen

Lúcio

Yamada

et al. 2021

J Infect Dev Ctries

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Introduction: Acinetobacter baumannii complex are microorganisms of critical priority of resistance, being associated with higher costs and negative outcomes for hospitalized patients. Thus, the study aimed to analyse the factors associated with A. baumannii complex infection in various hospital sectors. Methodology: This is a case-control study that included patients hospitalized from January 2017 to June 2019. Demographic, microbiological and clinical variables were collected from each patient. All cases had positive culture results for A. baumannii complex resistant to more than three classes of antimicrobials. Carbapenem-resistance was examined by the disk diffusion test, while the broth microdilution method was used to determine the susceptibility to colistin. Results: A. baumannii complex infection was mostly present in ICU (74.2%) than in other hospital areas. The bacteria was also linked with the length of hospitalization until the results for the culture (OR = 1.13; 95% CI: 1.06 – 1.21; p < 0.001) and with pneumonia associated with mechanical ventilation (OR = 4.48; 95% CI: 1.55 – 13.00; p = 0.006). Moreover, patients exposed to infection with multidrug-resistant A. baumannii complex had higher risks of death (OR = 3.25; 95% CI: 1.06 – 9.91; p = 0.039). Conclusions: This study provides evidence that A. baumannii complex infection is associated with the number of days of hospitalization up to culture positivity, pneumonia associated with the use of mechanical ventilation and death. Infections appear to be more critical in ICU when compared to other areas. Taken together, these findings could support hospital infection surveillance programs, as well as prevention measures to reduce mortality rates and other complications.

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Section: Discussionsupporting

confidence: 89%

Associated factors of Acinetobacter baumannii complex in hospitalized patients: A case-control study

Vandresen

Lúcio

Yamada

et al. 2021

J Infect Dev Ctries

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“…In 72% of the strains both of the quorum sensing genes was observed. In a previous study, using the same strains, the quick biofilm production were evaluated in 4h/ at 37 o C, besides in 24h/25 o C, 24h/37 o C and 48h/25 o C [13]. In this present study we also try to find an association between biofilm productions found before with the presence of the genes researched now.…”

Section: Detection Of Genes and Association To The Pathogenicitymentioning

confidence: 68%

“…The genetic diversity among the strains was evaluated by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR, and phenotyping assays of biofilm formation on polystyrene microplate. All these tests were realized in previous study of the same research group [13].…”

Section: Acinetobacter Baumannii Strains Characterizationmentioning

confidence: 99%

“…The induction of apoptosis in macrophages cells by A. baumannii was analyzed according to protocols published elsewhere [14]. These assays were performed with 20 studied strains: 19 strains that presented intergenic regions with more than 50% similarity by ERIC-PCR analyzes (date not shown) [13] and the reference (A. baumannii ATCC 19606).…”

Section: Evaluation Of Cell Death Induced By a Baumannii Strainsmentioning

confidence: 99%

“…Nineteen clinical strains were selected for this test, based on ERIC-PCR analyzes previously realized [13], presenting at least 50% of similarity. Bacterial strains were thawed and incubated in 5mL of Tryptic Soy Broth (DIFCO ® ) medium for 24h.…”

Section: Preparation a Baumannii Strains For Infectionmentioning

confidence: 99%

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Investigation of Relevant Genes and Pathogenicity Factors in Multidrug-Resistant Acinetobacter Baumannii Strains Isolated from Clinical Specimens

Costa¹,

Martins²,

Farias³

et al. 2020

Research Trends of Microbiology

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Acinetobacter baumannii has become a clinical and epidemiological relevant microorganism word wide. Several studies reveal aspects important to this pathogen to cause infectious disease, such as membrane proteins apoptosisinducing, biofilm formation, resistance to oxidative stress, besides of their potential to acquire antimicrobial resistance genes. However, the mechanisms underlying the success of this pathogen remain of great interest. This study evaluated some pathogenicity factors presented by strains of Multidrug-Resistant (MDR) A. baumannii isolated from different clinical specimens of patients in Belo Horizonte city, Brazil. Genes associated to the capsule, biofilm, apoptosisinducing and quorum sensing were researched by Polymerase Chain Reaction. The hemolytic activity of the strains in six blood types from three different mammalian species (sheep, horse and human blood type A, B, O, AB, all Rh positive) and the sensitivity to oxidative stress by hydrogen peroxide occurred by disc diffusion test. In vitro apoptosis was evaluated in bone marrow macrophages obtained from BALB/c mice. Overall, patients had mean age of 60.6 years (± 17.6) and most of them (95%) were using some invasive device. All strains (29) were positive for gaiU and bap, 76% to wzc, 86% to luxI and 79% luxR, 45% to omp33 and 31% to ompA genes. In the oxidative stress test, 49% were more resistant (p<0.05) than the reference strains. In relation to in vitro tests, eight strains led to a significant reduction in the number of macrophages as compared to controls (p <0.05), among them 63% was positive to omp33 and ompA genes. The results show significant pathogenicity factors that contribute to the colonization and to the success of an infectious disease in hospitalized patients. Therefore, these data contributed to confirm the potential for aggression by these microorganisms, in addition to alerting to the need for constant improvements in nosocomial infection control protocols.

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Mechanisms involved in resistance to carbapenems among Acinetobacter baumannii isolates recovered in Brazil: A systematic review and meta-analysis

Pieve de Castro,

Gustavo de Lima,

Sanches

et al. 2022

Rev. Colomb. Cienc. Quím. Farm.

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Introduction: Infections caused by carbapenem-resistant Acinetobacter baumannii(CRAB) is a health problem due to the limited therapeutic options available. This study was carried out to evaluate the main mechanisms of resistance of carbapenems in CRAB in the last 10 years in Brazil and to describe the susceptibility profile to tigecycline and polymyxins in these isolates. Material and methods: A systematic review was carried out according to Prisma in PUBMED/MEDLINE, Scopus, SciELO, Biblioteca Virtual de Saúde (BVS) and Cochrane Library. Data regarding enzyme resistance to carbapenems were evaluated by meta-analysis according to the random effect. Results: 21 articles were selected according to inclusion and exclu-sion criteria that evaluated 1096 CRAB. Most of the studies were carried out in the southern (33.3 %) and southeast (23.8 %) regions of Brazil (33.3 %) and in 2016 and 2018. According to the meta-analyzes, OXA-type carbapenemase was the main mechanism involved in the low susceptibility to carbapenems in CRAB (98%; 95% CI: 0.91, 0.99; I² = 95%), with blaOXA-23-like(91 %; 95 % CI: 0.76; 0.97; I² = 97 %) or blaOXA-51-like/ ISAba1 (84 %; 95 % CI: 0.15, 0.99; I² = 98 %) genes, followed by metallo-β-lactamases (MBL) (12 %, 95 % CI: 0.09, 0.15, I² = 99 %) and Klebsiella pneumoniae carbapenemase (KPC) (6 %, 95 % CI: 0.04; 0.08; I² = 87 %). Conclu-sion: The included studies showed that susceptibility to colistin (99 %) and tigecy-cline (93 %) remains high and was not affected by carbapenem resistance.

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Molecular association of pathogenicity and resistance to multiple antimicrobials in Acinetobacter baumannii strains recovered from patients with diverse infectious diseases

Cited by 4 publications

References 0 publications

Associated factors of Acinetobacter baumannii complex in hospitalized patients: A case-control study

Associated factors of Acinetobacter baumannii complex in hospitalized patients: A case-control study

Investigation of Relevant Genes and Pathogenicity Factors in Multidrug-Resistant Acinetobacter Baumannii Strains Isolated from Clinical Specimens

Mechanisms involved in resistance to carbapenems among Acinetobacter baumannii isolates recovered in Brazil: A systematic review and meta-analysis

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