Evaluation of MALDI-TOF mass spectrometry (VITEK-MS) compared to the ANC card
            (VITEK 2) for the identification of clinically significant anaerobes

Tsukimoto, Eliane R.; Rossi, Flávia

doi:10.5935/1676-2444.20180036

Cited by 3 publications

(4 citation statements)

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“…Fortunately, this was updated to the VITEK MS database 2.0, and interestingly 7/7 C . ramosum strains included in a recently published study were identified correctly [ 27 ].…”

Section: Discussion and Review Of Literaturementioning

confidence: 99%

“…However, most of the kits for biochemical identification of C. ramosum need to be supplemented with additional tests for the complete identification of the organism [3,18,25,26]. Further, ANC cards (VITEK 2, bioMérieux, France) have identified C. ramosum as C. clostridioforme [27]. For all these reasons, the 16S rRNA gene needs to be sequenced to confirm the identification [1,9].…”

Section: Case Presentationmentioning

confidence: 99%

“…Modern alternatives such as MALDI-TOF MS offer a fast, accurate, sensitive, robust and inexpensive solution for routine characterization and typing of microbes in laboratories. In fact, both VITEK MS (VITEK MS database 2.0, bioMérieux, France) and MALDI-TOF MS (Bruker Biotyper -Becton Dickinson) showed excellent performance for the identification of C. ramosum [27,29]. However, it is important to consider which database has been used in the identification process, because in a study carried out by Lee et al, 2/7 C. ramosum isolates were not identified by VITEK MS with the IVD 1.1 database [30].…”

Section: Case Presentationmentioning

confidence: 99%

“…However, it is important to consider which database has been used in the identification process, because in a study carried out by Lee et al, 2/7 C. ramosum isolates were not identified by VITEK MS with the IVD 1.1 database [30]. Fortunately, this was updated to the VITEK MS database 2.0, and interestingly 7/7 C. ramosum strains included in a recently published study were identified correctly [27].…”

Section: Case Presentationmentioning

confidence: 99%

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Clostridium ramosum rapidly identified by MALDI-TOF MS. A rare gram-variable agent of bacteraemia.

Legaria

García

Tudanca

et al. 2020

Access Microbiology

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Clostridium ramosum is an enteric anaerobic, endospore-forming, gram-positive rod with a low GC content that is rarely associated with disease in humans. We present a case of C. ramosum bacteraemia. To the best of our knowledge, this is the second case of C. ramosum bacteraemia in an elderly patient presenting with fever, abdominal pain and bilious emesis. We highlight the Gram stain variability, the lack of visualization of spores and the atypical morphology of the colonies that showed C. ramosum in a polymicrobial presentation that initially appeared to show monomicrobial bacteraemia. The microorganism was rapidly identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We present a comprehensive literature review of 32 cases of clinical infections by C. ramosum in which we describe, if available, sex, age, clinical symptoms, predisposing conditions, other organisms present in the blood culture, other samples with C. ramosum, identification methodology, treatment and outcome.

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“…Fortunately, this was updated to the VITEK MS database 2.0, and interestingly 7/7 C . ramosum strains included in a recently published study were identified correctly [ 27 ].…”

Section: Discussion and Review Of Literaturementioning

confidence: 99%

Section: Case Presentationmentioning

confidence: 99%

Section: Case Presentationmentioning

confidence: 99%

Section: Case Presentationmentioning

confidence: 99%

See 2 more Smart Citations

Clostridium ramosum rapidly identified by MALDI-TOF MS. A rare gram-variable agent of bacteraemia.

Legaria

García

Tudanca

et al. 2020

Access Microbiology

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Clostridium ramosum bacteremia: A case series at a general acute care hospital

Shinzato¹,

Yonaha²,

Oshiro³

et al. 2023

Journal of Infection and Chemotherapy

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Effects of Whey Protein Supplementation on Gut Microbiota of Wistar Rats with Valproic Acid-Induced Autism Symptoms

Jones Severino Vasconcelos,

Silva Frederico,

Sousa Alves

et al. 2023

Future Microbiol.

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Aim: To evaluate the effects of whey protein (WP) supplementation (1.24 mg/g, 24 days) in rats with autism spectrum disorder (ASD) induced by valproic acid (400 mg/kg, single dose). Materials & methods: Wistar rats (14 days old) were divided into four groups: control, ASD, ASD plus WP and WP. Results: WP increased bacterial diversity and the number of colonies. Bacteria from the Firmicutes phylum were predominantly found in the supplemented groups (p < 0.05). WP also improved the animals' memory in the Y-maze test and decreased the time that male animals spent in the ‘solitary chamber’ (p < 0.05). Conclusion: WP supplementation positively influenced gut microbiota, along with memory.

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Evaluation of MALDI-TOF mass spectrometry (VITEK-MS) compared to the ANC card (VITEK 2) for the identification of clinically significant anaerobes

Cited by 3 publications

References 0 publications

Clostridium ramosum rapidly identified by MALDI-TOF MS. A rare gram-variable agent of bacteraemia.

Clostridium ramosum rapidly identified by MALDI-TOF MS. A rare gram-variable agent of bacteraemia.

Clostridium ramosum bacteremia: A case series at a general acute care hospital

Effects of Whey Protein Supplementation on Gut Microbiota of Wistar Rats with Valproic Acid-Induced Autism Symptoms

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