Cell block specimens applied to diagnostic routine ofthyroid fine-needle aspiration biopsy

Mitteldorf, Cristina A.T.S.; Vilela, Rafael Sarlo; Barros, Ana Carolina; Coudry, Renata A.

doi:10.5935/1676-2444.20180009

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of different cytological preparations, including conventional smears and cell blocks, can be very helpful [ 56 ]. The importance of examining Papanicolaou- and Diff-Quik-stained smears has been previously stressed, and the morphological findings are complementary and confirmatory [ 19 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Novel Mutations in Pilomatrixoma, CTNNB1 p.s45F, and FGFR2 p.s252L: A Report of Three Cases Diagnosed by Fine-Needle Aspiration Biopsy, with Review of the Literature

Mitteldorf

Vilela

Fugimori

et al. 2020

Case Reports in Genetics

View full text Add to dashboard Cite

Pilomatrixoma (calcifying epithelioma of Malherbe) is an uncommon benign skin appendageal tumor that differentiates toward hair matrix cells. It is misdiagnosed in up to 75% of cases by nondermatologists. Although the histopathological findings are well recognized and characteristic, diagnosis by fine-needle aspiration biopsy may be quite challenging. Several reports have emphasized the challenges in cytodiagnosis of pilomatrixoma, leading to a false-positive diagnosis. The lesions may show avidity for fludeoxyglucose on positron emission tomography/computed tomography scan, raising concern of a possible malignant neoplasm. CTNNB1 mutations have been reported in a high percentage of pilomatrixomas. Expression of β-catenin, the protein encoded by CTNNB1, is also frequently observed. To determine if routine cytological specimens can be successfully used to perform additional investigation and support or confirm the diagnosis in three cases of pilomatrixoma, we performed molecular analysis and immunohistochemistry to search for CTNNB1 mutation and β-catenin, respectively. β-Catenin positivity by immunohistochemistry was observed in basaloid cells in all three cases. Exon 3 mutations in CTNNB1 were detected in all cases. In addition, we detected a fibroblast growth factor receptor 2 (FGFR2) mutation in one of the cases. We reviewed the literature and present the clinical and morphological characteristics that must be considered along with other findings to accurately achieve the correct diagnosis, in correlation with the results of the ancillary technique. In conclusion, routine cytological specimens can be successfully used to perform additional investigations and support cytodiagnosis in difficult cases.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Ultrasound-guided punctures were performed through three passes per nodule in all cases. We systematically used different cytological preparations from the aspirated samples, including conventional smears and liquid-based and cell block specimens [ 56 ].…”

Section: Case Presentationmentioning

confidence: 99%

Novel Mutations in Pilomatrixoma, CTNNB1 p.s45F, and FGFR2 p.s252L: A Report of Three Cases Diagnosed by Fine-Needle Aspiration Biopsy, with Review of the Literature

Mitteldorf

Vilela

Fugimori

et al. 2020

Case Reports in Genetics

View full text Add to dashboard Cite

show abstract

“…Two cases showed no follicular cells. [10] www.pacificejournals.com/apalm eISSN: 2349-6983; pISSN: 2394-6466…”

Section: [Figures 1-6]mentioning

confidence: 99%

A Study to Assess the Utility of Cell Block Morphology and Immuno-Cytochemistry For Improving the Pre-Operative Diagnosis of Nodular Thyroid Lesions

Mishra

Das

et al. 2020

Ann of Pathol and Lab Med

View full text Add to dashboard Cite

Background: Thyroid nodules are quite common in the general population of India with increasing incidence of malignancy worldwide. Fine Needle Aspiration [FNA] has been the primary modality of pre-operative diagnosis of such lesions till date. But it has got its own share of fallacies and pit-falls. Cell-block [CB] can be done in the same sitting and it gives the advantage of architectural assessment with easy subjectivity to immuno-cytochemical [ICC] staining. Material and methods: FNA was done in all patients and CB was prepared by formalin method. ICC stains were used in cases with provisional diagnosis of malignancy and in all follicular neoplasm cases. SPSS v.20 was used for analysis. Sensitivity and specificity of cell block was calculated. Diagnostic correlation of both FNA and CB with respect to histopathological examination was done by using student t-test with confidence interval of 95%. Results: CB showed a correlation coefficient of 0.704 and a p value of 0.0001while FNA showed a correlation coefficient of .464 and a p-value of .001. So, CB showed a strong positive correlation and a high statistical significance. Cell block helped in diagnosing almost all the cases showing discordance in FNA except that of papillary carcinoma where the cellular yield was poor due to cystic degeneration. Sensitivity of cell block was 89.5% and Specificity was 96.9%. Conclusions: Cell block is definitely a good ancillary examination for thyroid nodular lesions in addition to FNA. It should be routinely performed in all thyroid cases as it improves the diagnostic yield and accuracy of cytological diagnosis.

show abstract

The diagnostic utility of cell block as an adjunct to routine cytology

Pathak,

Rhutso,

Ahmed

2024

ACHR

View full text Add to dashboard Cite

Despite the diagnostic utility of cell block in FNAC and fluid cytology, known for better cellular yield and improved diagnostic accuracy, the technique remains to be underutilized for diagnosing neoplastic lesions.In this paper, a hospital based prospective study of 195 samples was conducted on cytological samples with the aim (a) to evaluate the diagnostic utility of cell block as an adjunct to routine cytological evaluation of aspiration and effusion fluid specimens, (b) To correlate the findings of routine cytology, cell block and routine histopathological examination wherever possible and (c) to explore the possibility of using ancillary techniques such as immunohistochemistry on cell blocks. The study revealed, most of the patients were between 51- 60 years with female preponderance. Among 195 samples, 79 (40.5%) were peritoneal fluids and 85 (43.5%) were lymph node. The diagnosis on conventional smear and cell block showed 71 (36.5%) cases and 68 (35%) cases were negative for malignancy respectively. 6 (3%) cases and 4 (2%) cases were suspicious for malignancy respectively. 118 (60.5%) cases and 123 (63%) cases were positive for malignancy respectively. Amongst the peritoneal effusion the most common primary site was ovary whereas majority of the primary site was unknown in lymph node FNAC. Peritoneal effusion showed 8 additional cases as positive for malignancy in CB preparation which were negative or suspicious for malignancy on CS. In FNAC, two additional positive case was found in CB preparation of lymph node. A Kappa value of 89.5 % for statistical correlation between Conventional smear and cell block preparation was calculated. The use of Cell block technique increases the detection of malignancy when used as an adjunct to conventional smears. Cell block technique is simple, inexpensive and reliable adjuvant to smears and it is recommended for routine cytological diagnosis and for application of immunomarkers and molecular studies.

show abstract

Cell block specimens applied to diagnostic routine ofthyroid fine-needle aspiration biopsy

Cited by 3 publications

References 0 publications

Novel Mutations in Pilomatrixoma, CTNNB1 p.s45F, and FGFR2 p.s252L: A Report of Three Cases Diagnosed by Fine-Needle Aspiration Biopsy, with Review of the Literature

Novel Mutations in Pilomatrixoma, CTNNB1 p.s45F, and FGFR2 p.s252L: A Report of Three Cases Diagnosed by Fine-Needle Aspiration Biopsy, with Review of the Literature

A Study to Assess the Utility of Cell Block Morphology and Immuno-Cytochemistry For Improving the Pre-Operative Diagnosis of Nodular Thyroid Lesions

The diagnostic utility of cell block as an adjunct to routine cytology

Contact Info

Product

Resources

About