Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates

Lima, Janaina Sousa de; Braga, Kaoane Regina G. S.; Vieira, Camila Aoyama; Souza, Werika Weryanne Rosa de; Chávez-Pavoni, Juliana Helena; Araújo, Claudinéia de; Goulart, Letícia Silveira

doi:10.5935/1676-2444.20180006

Cited by 10 publications

(11 citation statements)

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“…In the current study, the phospholipase activity (Pz) was observed in 268 (89.3%) isolates of the total isolates, while 138 (46%) of these strains showed a higher Pz value of ≤ 0.79 mm (Table 3). These findings simulate with preceding studies where phospholipase activity was stated in 75% to 100% isolates of C. albicans isolated from varied origin sites and from several types of patients 8 . According to research conducted in 2014, 87.7% of the isolates showed phospholipase activity 26.…”

Section: Discussionsupporting

confidence: 92%

“…The Secreted Aspartyl Proteinases (SAPs) released from C. albicans can hydrolyze different types of human proteins on the injury site like hemoglobin, albumin, keratin, collagen, secretory immunoglobulin A (IgA-s), endothelial cell proteins, extracellular matrix proteins, mucin, different molecules in the immune system, various clotting and coagulation elements. This proteolytic activity has been linked to tissue incursion, and thus it causes tissue penetration by Candida species 7,8 .…”

Section: Introductionmentioning

confidence: 99%

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Assessment of different Enzymatic Virulence Traits contributing to pathogenicity of Candida Albicans

Nazeer

Naz

Zubair

et al. 2023

Int. j. endorsing health sci. res.

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Background: Candidiasis is regarded as one of the frequently occurring fungal infections of humans elicited by yeast that belongs to the genus Candida. Among the different species of Candida, Candida albicans is the most pathogenic specie. The pathogenicity of this specie depends on various virulence factors. This study was designed to determine the activity of some important enzymatic virulence factors such as phospholipase, proteinase and hemolysin produced by Candida albicans strains. Methodology: A total of 300 clinical isolates of C. albicans were obtained and identified by conventional standard diagnostic criteria. For the evaluation of phospholipase production, the egg yolk agar medium was employed. The minimum agar medium was used for proteinase activity and for hemolysin production, blood plate assay was used. The results were statistically analyzed by SPSS 16.0 version software. Results: Of a total of 300 isolates reviewed, the majority of the isolates were recovered from females, i.e. 234 (78%); however, the number of isolates obtained from males were 66 (22%). Amongst all the strains of C. albicans, phospholipase and hemolysin enzymes were produced primarily by 89.3% of the total C. albicans strains. While proteinase enzyme was produced by 86% of the total C. albicans isolates. A p-value < 0.05 was measured as statistically significant. Conclusion: From the existing study, it can be determined that these enzymes are produced by most of the pathogenic strains of C. albicans. The detection of these virulence enzymes is tremendously significant in designing of drugs to combat these opportunistic infections in future.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Assessment of different Enzymatic Virulence Traits contributing to pathogenicity of Candida Albicans

Nazeer

Naz

Zubair

et al. 2023

Int. j. endorsing health sci. res.

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“…SAP2 and 3 expressions in our study were similar to a previous report for C. albicans strains with oral and vaginal source by Dabiri et al [ 18 ] in Iran, whereas the result for SAP1 activity was different. According to a previous report by Lima et al [ 30 ] , the SAP1 gene had more expression in the process of vaginal infection in comparison to the oral infection. More investigation suggested that SAP1-3 appears to be essential for mucosal and SAP4-6 for systemic infections [ 31 ] .…”

Section: Discussionmentioning

confidence: 93%

In vivo and in vitro Pathogenesis and Virulence Factors of Candida albicans Strains Isolated from Cutaneous Candidiasis

Sadeghi

Mousavi

Ebrahimi‐Rad

et al. 2020

ibj

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Background: The Candida albicans is one of the most important global opportunistic pathogens, and the incidence of candidiasis has increased over the past few decades. Despite the established role of skin in defense against fungal invasion, little has been documented about the pathogenesis of Candida species when changing from normal flora to pathogens of vaginal and gastrointestinal epithelia. This study was carried out to determine the in vivo and in vitro pathogenesis of clinical C. albicans strains isolated from skin lesions. Methods: In this study, association of in vivo and in vitro pathogenesis of C. albicans isolates with different evolutionary origins was investigated. Oral and systemic experimental candidiasis was established in BALB/C mice. The expression levels of SAP1-3 genes, morphological transformation, and biofilm-forming ability of C. albicans were evaluated. Results: All the strains showed in vitro and in vivo pathogenicity by various extents. The SAP1, SAP2, and SAP3 genes were expressed in 50%, 100%, and 75% of the strains, respectively. The biofilm formation ability was negative in 12% of the strains, while it was considerable in 38% of the strains. Fifty percent of the strains had no phospholipase activity, and no one demonstrated high level of this pathogenesis factor. Relatively all the strains had very low potency to form pseudohyphae. Conclusion: Our findings demonstrated that Candida albicans strains isolated from cutaneous candidiasis were able to cause oral and systemic infections in mice, so they could be considered as the potential agents of life-threatening nosocomial candidiasis in susceptible populations.

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“…are commonly associated with diseases in humans, such as oral and vulvovaginal candidiasis, skin infections, and onychomycosis. These infections may occur even in immunocompetent hosts [ 4 , 5 ], when predisposing factors are present (age, antibiotic use, sexual activity, diabetes mellitus, and idiopathic causes), resulting in the possible need for prolonged treatments that may cause recurrences and generation of resistant species [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Ethyl Acetate Fraction of Punica granatum and Its Galloyl-HHDP-Glucose Compound, Alone or in Combination with Fluconazole, Have Antifungal and Antivirulence Properties against Candida spp.

et al. 2022

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Candidiasis is the most common fungal infection among immunocompromised patients. Its treatment includes the use of antifungals, which poses limitations such as toxicity and fungal resistance. Plant-derived extracts, such as Punica granatum, have been reported to have antimicrobial activity, but their antifungal effects are still unknown. We aimed to evaluate the antifungal and antiviral potential of the ethyl acetate fraction of P. granatum (PgEA) and its isolated compound galloyl-hexahydroxydiphenoyl-glucose (G-HHDP-G) against Candida spp. In silico analyses predicted the biological activity of G-HHDP-G. The minimum inhibitory concentrations (MIC) of PgEA and G-HHDP-G, and their effects on biofilm formation, preformed biofilms, and phospholipase production were determined. In silico analysis showed that G-HHDP-G has antifungal and hepatoprotective effects. An in vitro assay confirmed the antifungal effects of PgEA and G-HHDP-G, with MIC in the ranges of 31.25–250 μg/mL and 31.25 ≥ 500 μg/mL, respectively. G-HHDP-G and PgEA synergistically worked with fluconazole against planktonic cells. The substances showed antibiofilm action, alone or in combination with fluconazole, and interfered with phospholipase production. The antifungal and antibiofilm actions of PgEA and G-HHDP-G, alone or in combination with fluconazole, in addition to their effects on reducing Candida phospholipase production, identify them as promising candidates for therapeutics.

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Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates

Cited by 10 publications

References 0 publications

Assessment of different Enzymatic Virulence Traits contributing to pathogenicity of Candida Albicans

Assessment of different Enzymatic Virulence Traits contributing to pathogenicity of Candida Albicans

In vivo and in vitro Pathogenesis and Virulence Factors of Candida albicans Strains Isolated from Cutaneous Candidiasis

Ethyl Acetate Fraction of Punica granatum and Its Galloyl-HHDP-Glucose Compound, Alone or in Combination with Fluconazole, Have Antifungal and Antivirulence Properties against Candida spp.

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