Pseudothrombocytopenia: incidence and strategy for resolution in clinical
            laboratory

Prates, Romário Farias; Viana, Robson C.; Oliveira, Márcio Vasconcelos; Souza, Cláudio Lima

doi:10.5935/1676-2444.20170061

Cited by 2 publications

(4 citation statements)

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“…None of the mentioned guidelines describe how to quantify aggregation, and not all guidelines have a precise definition of aggregation. Accordingly, previous studies of PTCP from hematology instruments have used various or unspecified definitions of aggregation, raising the concern that the studies may not be comparable 6–10 …”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, previous studies of PTCP from hematology instruments have used various or unspecified definitions of aggregation, raising the concern that the studies may not be comparable. [6][7][8][9][10] The aim of this study was to investigate the diagnostic accuracy of the flag PLT-Clumps from the WNR/WDF and the PLT-F channels from Sysmex XN. Furthermore, we aimed to study how different cutoffs for investigation for PTCP and different definitions of platelet aggregation affected the diagnostic accuracy.…”

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confidence: 99%

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The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation

Lunde

Hjelmtvedt

Amundsen

2022

Int J Lab Hematology

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Introduction:The aim of this study was to investigate the diagnostic accuracy of the flag PLT-Clumps from the WNR/WDF and the PLT-F channels from Sysmex XN and to study how different cut-offs for investigation for pseudothrombocytopenia (PTCP) and the definition of platelet aggregation affected the diagnostic accuracy.Methods: A smear review was performed for samples with platelet count <150 Â 10 9 /L and samples flagged for platelet aggregation by Sysmex XN-20. The samples were investigated for platelet aggregation in 30 fields using 40Â objective. Findings were classified by size and quantity using two definitions of aggregation: the Norwegian quality improvement of laboratory investigations (Noklus) and the Groupe Francophone de'Hematologie Cellulaire (GFHC). The Q-values for the PLT-Clumps flag from the WNR/WFD channel and the PLT-F channel were compared with smear findings.Results: ROC analysis showed that the diagnostic accuracy of the PLT-clumps flags increased with increasing stringency of the definition of platelet aggregation and when only samples with thrombocytopenia were investigated. With the most stringent PTCP definitions, the diagnostic accuracy of the PLT-Clumps flag from PLT-F was very high (AUC 0.97-0.98) and markedly better than for WNR/WDF. Conclusion:The diagnostic accuracy of PLT-F from Sysmex XN-20 for identification of PTCP was very good and superior to the WNDR/WDF channel in samples with platelet count <150 Â 10 9 /L and a moderate to high number of aggregates in the smear. There is a need for a more precise definition of platelet aggregation.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation

Lunde

Hjelmtvedt

Amundsen

2022

Int J Lab Hematology

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“…In most European countries, the most common formulation used is K2EDTA. EDTAdependent pseudothrombocytopenia (EDTA-PTCP) is caused by EDTA-depentent antiplatelet IgM and IgG antibodies which recognize cytoadhesive glycoprotein IIb/IIIa receptors on the platelet plasma membrane, and it occurs at room temparature (2). EDTA-PTCP is a rare phenomenon with overall prevalence of 0.1% in the general population and is not sex-or age-dependent.…”

Section: Introductionmentioning

confidence: 99%

“…Patients with infection, autoimmune diseases and malignancy might have an increased risk of EDTA-PTCP. However, it was recorded in a disease-free population as well (2,3). This phenomenon ocurrs only in vitro and is solely a laboratory artifact because with a different antico-agulant used, the patient presented the physiological platelet count (4).…”

Section: Introductionmentioning

confidence: 99%

Pseudothrombocytopenia induced by ethylenediaminetetraacetic acid: A case report

Dobrijević¹,

Kaćanski-Rađenović²,

Katanić³

et al. 2022

Acta fac medic Naissensis

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Introduction: Pseudothrombocytopenia induced by ethylenediaminetetraacetic acid is a rare phenomenon caused by autoantibodies against platelet membrane with overall prevalence of 0.1% in the general population. This phenomenon is an in vitro artifact and with different anticoagulant used, the patient had the physiological platelet count. Case report: This case is a report of a 2-year-old patient, whose initial complete blood count analysis revealed low platelet count (18 x 10 9 /L) without any visible signs of hemorrhage. After detecting multiple platelet clumps in the peripheral blood smear, the complete blood count was repeated using 3.2% sodium-citrate. The analysis of the new specimen revealed the physiological platelet count. Thus, the patient was diagnosed with pseudothrombocytopenia induced by ethylenediaminetetraacetic acid. Conclusion: Pseudothrombocytopenia induced by ethylenediaminetetraacetic acid should always be suspected whenever thrombocytopenia without bleeding tendency is presented. The peripheral blood smear examination is the most convenient and the cheapest diagnostic test for excluding this artifact.

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Pseudothrombocytopenia: incidence and strategy for resolution in clinical laboratory

Cited by 2 publications

References 0 publications

The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation

The diagnostic accuracy of Sysmex XN for identification of pseudothrombocytopenia using various thresholds for definition of platelet aggregation

Pseudothrombocytopenia induced by ethylenediaminetetraacetic acid: A case report

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