Frequency of classes I and II HLA alleles in deceased donors of solid organs in
            Espírito Santo, Brazil

Barbosa, Fabiana Regina; Freitas, Karla O.; Silva, Rafaela C.; Pires, Sarah P. C.; Pratte-Santos, Rodrigo

doi:10.5935/1676-2444.20170047

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, two peptides showed satisfactory affinity for the HLA-DRB1 * 15: 01 allele––RAYALLFSKLGAAVV and ERVSIIANPAVASLY. Thus, based on the knowledge that the most frequent alleles in the Brazilian population are HLA-DRB1 * 15: 01, HLA-DRB1 * 07: 01, and HLA-DRB1 * 03: 01, the four peptides found exhibited appropriate coverage for the Brazilian population [ 62 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy

Rosa

Csordas

Oliveira

et al. 2020

JoF

View full text Add to dashboard Cite

Impaired antigen-specific cell-mediated immunity (CMI) is a primary immunological disturbance observed in individuals that develop paracoccidioidomycosis (PCM) after exposure to Paracoccidioides spp. Restoration of Paracoccidioides-specific CMI is crucial to stop the antifungal treatment and avoid relapses. A convenient and specific laboratory tool to assess antigen specific CMI is required for the appropriate clinical treatment of fungal infections, in order to decrease the time of antifungal therapy. We used an interferon-γ release assay strategy, used in the diagnosis of latent tuberculosis infection, to address our aims in this study. Information on proteins secreted by two well-studied representative strains—Paracoccidioides brasiliensis (Pb18) and P. lutzii (Pb-01)—were explored using PubMed or MEDLINE. From 26 publications, 252 proteins were identified, of which 203 were similar according to the Basic Local Alignment Search Tool. This enabled a selection of conserved peptides using the MEGA software. The SignalP-5.0, TMHMM, IEDB, NetMHC II, and IFNepitope algorithms were used to identify appropriate epitopes. In our study, we predicted antigenic epitopes of Paracoccidioides that could bind to MHC class II and induce IFN-γ secretion. These T cell epitopes can be used in the development of a laboratory tool to monitor the CMI of patients with PCM.

show abstract

Section: Discussionmentioning

confidence: 99%