Evaluation of pleural and ascitic fluid analysis on the Sysmex XE-5000 hematology
            analyzer

Cognialli, Regielly Caroline Raimundo; Comar, Samuel Ricardo; Souza, Angela Maria de; Singer, Gisele M. B.

doi:10.5935/1676-2444.20170023

Cited by 2 publications

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“…In terms of false negatives, there were 7 out of 50 cases; majority of them were CSF samples that have low cell yield. There was no carry over of WBC in this study as reported by Caroline et al 9 The advantage of automated BF analyzer could not be undermined as the analysis is quick and reliable as stressed by Caroline et al 29 and Aguadero et al 30 We acknowledge that the limited sample size and inability to capture time interval between sample collection and analysis were limitations of the study. It mandates that in the absence of clear-cut discriminatory value for HF cells to detect malignant cells, each laboratory should establish its own cutoff in BF analysis on automated analyzer.…”

Section: Discussionmentioning

confidence: 53%

High Fluorescent Cells on Automated Body Fluid Analysis as Discriminator for Malignant Cell Detection

Saini,

Sareen,

Gupta

2023

South Asian J Cancer

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The automated examination of body fluids (BF) serves as a valuable screening tool for the presence of malignant cells in such samples. Malignant cells are identified as high fluorescence cells (HFC) when analyzed using the Sysmex XN-1000 automated analyzer. This study aimed to assess the correlation between HFC cell counts generated by the automated analyzer and manual cytological examination for detecting malignant cells. Additionally, it sought to establish reliable cutoff values for malignant cells since there is a lack of literature on this subject. Conducted at the department of pathology hematology and cytology laboratory in a tertiary care hospital in India from January 2019 to May 2020, this hospital-based comparative study analyzed 120 BF samples, each subjected to cytological evaluation. The mean age of the study population was 52 years, with 70 male and 50 female patients (male-to-female ratio of 1.4:1). The samples consisted of 53 ascitic fluids (44.17%), 46 pleural fluids (38.33%), and 21 cerebrospinal fluids (CSF; 17.50%). Cytopathological examination revealed malignant cells in 50 (41.67%) of the BF samples, with 70 (58.33%) samples classified as nonmalignant. Specifically, among the ascitic fluids, 24 (48%) were malignant, while 29 (41.43%) were nonmalignant. For pleural fluids, 24 (48%) were malignant, and 22 (31.43%) were nonmalignant. In CSF, 2 (4%) samples were malignant, and 19 (27.14%) were nonmalignant. The total white blood cell counts provided by automated hematology analyzers were significantly higher in malignant samples, ranging from a minimum of 100 cells to a maximum of 60,000, with a median count of 800. Nonmalignant samples had white blood cell counts ranging from 2 to 12,000, with a median count of 100. Subgroup analysis for ascitic, pleural, and CSF samples revealed significantly higher median HFC counts in malignant samples. Receiver operating characteristic curve analysis indicated that the HF-BF parameter could effectively distinguish between benign and malignant fluids. For HF#, the area under the curve (AUC) was 0.844, with a sensitivity of 82% and specificity of 81%, while HF% had an AUC of 0.706, with sensitivity and specificity values of 72% and 72.9%, respectively. This study highlights that the HFC count in the BF mode of Sysmex XN-1000 can be a valuable tool for predicting the presence of malignant cells in serous fluids and for selecting samples for further microscopic examination. Based on this study, cutoff values of 15.70/µL for absolute HFC count and 5.05% for relative HFC count can be applied to screen BF samples for malignancy, offering good sensitivity and specificity.

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Section: Discussionmentioning

confidence: 53%

High Fluorescent Cells on Automated Body Fluid Analysis as Discriminator for Malignant Cell Detection

Saini,

Sareen,

Gupta

2023

South Asian J Cancer

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“…Total WBC count in body fluids (BF) is important in diagnosis and management of neoplasms, hemorrhages, inflammatory and infectious diseases 1,2 .…”

Section: Introductionmentioning

confidence: 99%

A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids

Savcı¹,

Şahin²,

Eser³

2020

Dicle Tıp Dergisi

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Total white blood cell (WBC) count in body fluids (BF) is important in diagnosis and treatment of inflammatory and infectious diseases. We aimed to compare hemogram and BF modes of Mindray BC-6800 hematology analyzer with the manual hemocytometry method for determination of total WBC count in peritoneal and pleural fluids. Method:Our study consisted of a total of 143 specimens, 109 peritoneal fluid and 34 pleural effusion fluid. Each sample was analyzed consecutively twice, first with the manual method and, later with both hemogram mode and the BF mode of Mindray BC-6800 automated hematology analyzer.Results: There was a statistically significant difference between manual count and hemogram mode (for peritoneal fluids p< 0.001, for pleural fluids p< 0.001). There was a statistically significant difference between the hemogram mode and BF mode too. (for peritoneal fluids p< 0.001, for pleural fluids p< 0.001). However, there was no statistically significant difference between the manual count and BF mode (for peritoneal fluids p=0.236, for pleural fluids p=0.627). Less than100 cells/mL, there was a statistically significant difference between each of the counting methods (Manual count vs hemogram mode p< 0.001, manual count vs BF mode p= 0.012, hemogram mode vs BF mode p< 0.001).

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Evaluation of pleural and ascitic fluid analysis on the Sysmex XE-5000 hematology analyzer

Cited by 2 publications

References 0 publications

High Fluorescent Cells on Automated Body Fluid Analysis as Discriminator for Malignant Cell Detection

High Fluorescent Cells on Automated Body Fluid Analysis as Discriminator for Malignant Cell Detection

A Comparison Of Manual Hemocytometry And Different Modes Of Mindray BC-6800 Hematology Analyzer For Cell Count In Peritoneal And Pleural Fluids

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