Performance validation of western blot for anti-HIV antibody detection in blood samples collected on filter paper (DBS)

Castejon, Márcia Jorge; Yamashiro, Rosemeire; Oliveira, Camila Cardoso de; Veras, Maria Amélia

doi:10.5935/1676-2444.20170002

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method is also used to examine protein expression [72][73][74]. Furthermore, this technique has been employed in developing serological assays to detect antiviral antibodies in patients' sera, e.g., WB-based serological tests for human immunodeficiency virus (HIV), influenza A and B viruses, and CoVs [57,75,76].…”

Section: Western Blot (Wb)mentioning

confidence: 99%

Serological assays and host antibody detection in coronavirus-related disease diagnosis

2021

View full text Add to dashboard Cite

Coronaviruses (CoV) are a family of viral pathogens that infect both birds and mammals, including humans. Seven human coronaviruses (HCoV) have been recognized so far. HCoV-229E, -OC43, -NL63, and -HKU1 account for one-third of common colds with mild symptoms. The other three members are severe acute respiratory syndrome (SARS)-CoV, Middle East respiratory syndrome (MERS)-CoV, and SARS-CoV-2. These viruses are responsible for SARS, MERS, and CoV disease 2019 (COVID-19), respectively. A variety of diagnostic techniques, including chest X-rays, computer tomography (CT) scans, analysis of viral nucleic acids, proteins, or whole virions, and host antibody detection using serological assays have been developed for the detection of these viruses. In this review, we discuss conventional serological tests, such as enzyme-linked immunosorbent assay (ELISA), western blot (WB), immunofluorescence assay (IFA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA), as well as biosensor-based assays that have been developed for diagnosing HCoV-associated diseases since 2003, with an in-depth focus on COVID-19.

show abstract

Section: Western Blot (Wb)mentioning

confidence: 99%

Serological assays and host antibody detection in coronavirus-related disease diagnosis

2021

View full text Add to dashboard Cite

show abstract

“…45°C and 50°C are stable for 8 and 4 weeks respectively when tested by rapid diagnostic kit [5]. By using western blotting technique, antibody against HIV from DBS can be detected with high sensitivity and specificity [6]. Another study suggested that HIV positive samples showed positivity when stored at 4°C for 14 years [7].…”

mentioning

confidence: 99%

Stability of Human Immunodeficiency Virus 1 and 2 Antibody in Dried Blood Spot: A Pilot Study

Saud

Masranggi

Paudel

et al. 2020

BJSTR

View full text Add to dashboard Cite

Dried Blood Spot (DBS) become a most proficient method of sample collection for both developed and developing countries. It is most commonly using to collect, store and transport sample for serological/immunological test, and pharmacokinetic studies, metabolic profiling etc. DBS has become an alternative tool for long-term storage, less hazardous and cost-effective method of sample handling. Thus, present study designed to investigate the stability of anti-HIV 1 and 2 antibodies in DBS in our environmental setting.

show abstract

Performance validation of western blot for anti-HIV antibody detection in blood samples collected on filter paper (DBS)

Cited by 2 publications

References 0 publications

Serological assays and host antibody detection in coronavirus-related disease diagnosis

Serological assays and host antibody detection in coronavirus-related disease diagnosis

Stability of Human Immunodeficiency Virus 1 and 2 Antibody in Dried Blood Spot: A Pilot Study

Contact Info

Product

Resources

About