Comparative study of five commercial probes for the detection of Epstein-Barr virus
            (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki’s lymphoma

Nonogaki, Suely; Shirata, Neuza Kasumi; Kimura, Lidia Midori; Guerra, Juliana Mariotti; Medeiros, Raphael Salles Scortegagna de; Paes, Roberto Pinto; Kanamura, Cristina Takami; Oliveira, Camila Carla Maria Ximenes; Menezes, Yara

doi:10.5935/1676-2444.20160065

Jornal Brasileiro De Patologia E Medicina Laboratorial

2016

DOI: 10.5935/1676-2444.20160065

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Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki’s lymphoma

Suely Nonogaki¹,

Neuza Kasumi Shirata²,

Lidia Midori Kimura³

et al.

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2018

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“…For high-quality (mainly molecular) examination, fixation, post-fixation processing, and preparation of sections must be the priority of pre-analysis team members. 10 Molecular tests are used for latent EBV infection diagnosis and include in situ hybridisation (ISH) and PCR. The membrane latency proteins can also be detected by IHC (Figure 1).…”

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Epstein–Barr Virus: A Biological Overview and Clinicopathological Changes of Two Epstein–Barr Virus-Related Lymphoproliferative Disorders in a World Health Organization (WHO) 2017 Report

Oliveira

2018

EMJ

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Epstein–Barr virus (EBV) is one of the most important viral causes for the development of tumours. The global geographical epidemiology of EBV shows prevalence differences between rich and poor countries across the world, and the impact on health suggests EBV should be an important target of research worldwide. This article will discuss the biology of the virus with an emphasis on its latency types, vital to understanding the possibilities of viral detection. The main objective is to discuss two lymphoproliferative diseases that are associated with EBV and appear in the World Health Organization (WHO) 2017 Classification of Tumours of Haematopoietic and Lymphoid Tissues: EBV-positive diffuse large B cell lymphoma and EBV mucocutaneous ulcer. The name of the former was changed to support the better understanding of infection pathology, while the second was recently described and made its debut in the WHO classification. Pathologists must have knowledge on these diseases and how to investigate them, and oncologists and clinical doctors must be informed on the guidelines.

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confidence: 99%

“…Thus, as discussed above, other proteins, including nuclear proteins, may be expressed. 10 Another method for EBV detection is through plasma and peripheral blood mononuclear cell (PBMC) analysis. Kanakry et al 11 studied the clinical significance of PBMC detection, using viral quantitative real-time PCR of plasma and PBMC.…”

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confidence: 99%

Epstein–Barr Virus: A Biological Overview and Clinicopathological Changes of Two Epstein–Barr Virus-Related Lymphoproliferative Disorders in a World Health Organization (WHO) 2017 Report

Oliveira

2018

EMJ

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Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki’s lymphoma

Cited by 1 publication

References 0 publications

Epstein–Barr Virus: A Biological Overview and Clinicopathological Changes of Two Epstein–Barr Virus-Related Lymphoproliferative Disorders in a World Health Organization (WHO) 2017 Report

Epstein–Barr Virus: A Biological Overview and Clinicopathological Changes of Two Epstein–Barr Virus-Related Lymphoproliferative Disorders in a World Health Organization (WHO) 2017 Report

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