Immunohistochemical study of macrophages subpopulations associated with squamous cell carcinoma of the tongue, with and without metastasis

Barbosa, Natália Guimarães; Sá, Melka Coelho; Pinto, Leão Pereira; Freitas, Roseana A.

doi:10.5935/1676-2444.20150064

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…M1 macrophages are classically considered potent effector cells that participate in proinflammatory processes. M2 macrophages, on the other hand, are characterized by their ability to inhibit the cytotoxic and inflammatory functions of M1 macrophages, while also being involved in angiogenesis, anti-inflammatory effects, tissue repair and remodeling, and fibrosis 11 . When macrophages are activated, they present increased cell size and lysosomal enzyme content, as well as a more active metabolism and greater ability to destroy endocytosed organisms 7 .…”

Section: Discussionmentioning

confidence: 99%

“…CD68 is a 110-K-Da intracellular glycoprotein that recognizes M1 and M2 macrophages due to its location in the primary granules of macrophage lysosomes, playing a role in endocytosis and lysosomal movement 11 . CD163 is a 130-k-Da glycoprotein belonging to the scavenger receptor group and is present, specifically, in M2 macrophages.…”

Section: Declaration Of Interestsmentioning

confidence: 99%

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Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

et al. 2019

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The aim of this study was to evaluate macrophage M1 and M2 subpopulations in radicular cysts (RCs) and periapical granulomas (PGs) and relate them to clinical and morphological aspects. M1 macrophages were evaluated by the percentage of CD68 immunostaining associated with the inflammatory cytokine TNF-α, and M2 macrophages, by its specific CD163 antibody. The CD68 + / CD163 + ratio was adopted to distinguish between the two macrophage subpopulations. Clinical, radiographic, symptomatology, treatment, and morphological parameters of lesions were collected and a significance level of p = 0.05 was adopted for statistical analysis. The results showed that the CD68 + /CD163 + ratio was higher in the RCs (median = 1.22, p = 0.002), and the highest TNF-α immunostaining scores were found in RCs (p = 0.018); in PGs, the CD68 + /CD163 + ratio was lower and associated with a greater CD163 + immunostaining (median = 1.02, p <0.001). The TNF-α in cyst epithelium had a score of 3 in 10 cases and predominance of M1 macrophages by CD68 + /CD163 + (median = 2.23). In addition, CD68 + cells had higher percentage of immunostaining in smaller RCs (p = 0.034). Our findings suggest that increased CD68 immunostaining associated with TNF-α cytokine in RCs results in a greater differentiation of the M1 phenotype. The higher CD163 immunostaining in PGs results in greater differentiation of the M2 phenotype. Therefore, the inflammatory state promoted by M1 macrophages is related to growth and progression of RCs; on the other hand, the immunomodulatory state of M2 macrophages is related to maintenance of PGs.

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Section: Discussionmentioning

confidence: 99%

Section: Declaration Of Interestsmentioning

confidence: 99%

Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

et al. 2019

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“…According to their functionality, M1 acts as a pro-inflammatory and tumorogenic macrophage, and M2 is regarded as the tissue repair macrophage [38,39]. CD68 antigen is expressed in both M1 and M2 populations, representing a classic sign of inflammation [40]; while CD163 is a specific phenotypic marker for M2 macrophage [41]. The histological results revealed a particularly important cellular reaction in the sham and antioxidant groups within the areas surrounding the scaffold.…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin Complexes to Attenuate Muscle Damage after In Vivo Femoral Ischemia-Reperfusion

et al. 2019

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(1) Background: Reperfusion injury refers to the cell and tissue damage induced, when blood flow is restored after an ischemic period. While reperfusion reestablishes oxygen supply, it generates a high concentration of radicals, resulting in tissue dysfunction and damage. Here, we aimed to challenge and achieve the potential of a delivery system based on astaxanthin, a natural antioxidant, in attenuating the muscle damage in an animal model of femoral hind-limb ischemia and reperfusion. (2) Methods: The antioxidant capacity and non-toxicity of astaxanthin was validated before and after loading into a polysaccharide scaffold. The capacity of astaxanthin to compensate stress damages was also studied after ischemia induced by femoral artery clamping and followed by varied periods of reperfusion. (3) Results: Histological evaluation showed a positive labeling for CD68 and CD163 macrophage markers, indicating a remodeling process. In addition, higher levels of Nrf2 and NQO1 expression in the sham group compared to the antioxidant group could reflect a reduction of the oxidative damage after 15 days of reperfusion. Furthermore, non-significant differences were observed in non-heme iron deposition in both groups, reflecting a cell population susceptible to free radical damage. (4) Conclusions: Our results suggest that the in situ release of an antioxidant molecule could be effective in improving the antioxidant defenses of ischemia/reperfusion (I/R)-damaged muscles.

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“…They promote inflammatory response (Wang et al, 2014) and may contribute to the establishment of lesions such as human prostate tumors (Fang et al, 2013). CD68 is a type I transmembrane glycoprotein and is a known pan‐macrophage marker (Kim et al, 2020) that can be utilized to recognize both tumoricidal M1 (Wang et al, 2020) and anti‐inflammatory M2 macrophages (He et al, 2014; Barbosa et al, 2015). This may explain a higher rate of macrophages/area obtained by using the CD68 marker, which may indicate not only the presence of M1 but M2 in the prostate.…”

Section: Discussionmentioning

confidence: 99%

“…The marker CD163 is specific only to identify M2 macrophages and has been associated with several tumor growth/progression and worse prognosis (Maniecki et al, 2012; Medrek et al, 2012; Palma and Lewis, 2013; Richards et al, 2013; He et al, 2014; Barbosa et al, 2015; Kim et al, 2020). The use of only this marker suggests classifying the cells marked as M2.…”

Section: Discussionmentioning

confidence: 99%

Ethinylestradiol and its effects on the macrophages in the prostate of adult and senile gerbils

Castro

Falleiros‐Júnior

Zucão

et al. 2020

Cell Biology International

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Prenatal and neonatal exposure to estrogenic compounds, such as ethinylestradiol (EE), promotes a variety of developmental disorders, including malformations and alterations in the morphology of glands, such as the prostate gland. Therefore, the aim of this study was to evaluate the morphological effects of neonatal exposure to EE on prostatic tissue and on the identification and quantification of gerbil gland macrophages in adult and senile Mongolian gerbils. The animals were exposed to EE (10 μg/kg/day) and to the vehicle, mineral oil (100 μL) (control group) during the first 10 days of postnatal life (lactation period). Adult gerbils were euthanized at 120 days and senile gerbils at 12 months of age. Our findings permitted verification of the presence of areas with proliferative foci in the prostate glandular portions in the adult and senile animals exposed to EE. There was also an increase in macrophages in the prostate tissue of adult and senile gerbils; these cell types alter the stromal microenvironment and possibly modify the interactions between the epithelium and stroma. Neonatal exposure to EE changes the pattern of prostatic development, leading to alterations in the arrangement of cells, including macrophages, and may be related to the onset of proliferative disorders in the prostate of adult gerbils and during aging.

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Immunohistochemical study of macrophages subpopulations associated with squamous cell carcinoma of the tongue, with and without metastasis

Cited by 6 publications

References 20 publications

Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

Astaxanthin Complexes to Attenuate Muscle Damage after In Vivo Femoral Ischemia-Reperfusion

Ethinylestradiol and its effects on the macrophages in the prostate of adult and senile gerbils

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