Evaluation of semi-automated cells counting in peritoneal fluid

Abstract

The history of muscle biopsy dates back to 1860, when Duchenne first performed a biopsy on a patient with symptoms of myopathy (1) . Since then, the basic and clinical science of muscle and muscle disease has gone through three stages of development: the classical period, the modern stage and the molecular era.

Evaluation of Automated Cell Counts in Body Fluids Using Sysmex Xt-4000i - An Important Need of the Day!

“…Similar results were observed by Daikelly et al and attributed to the equipment's inherent limitations. 2 CSF analysis of WBC counts by manual and automated methods showed a less agreement with correlation coefficient of 0.3. The same was observed by Paris and Linda et al 3,7 The number of cells counted in CSF samples were less and did not exceed 100 with both the methods when sample was clear.…”

“…6 While in the study by Daikelly et al, the analyzer detected small numbers of cells when compared to manual counts. 2 The correlation coefficient for the entire range of data was 0.99. This closely agreed with the study conducted by Linda et al, in which she obtained a correlation coefficient of 0.77 with study sample constituting 187 BFs.…”

“…1 Routine laboratory investigations of body fluids include physical, cytological, biochemical and bacteriological analysis which enable differentiation of the fluid between exudates and transudates and detection of infections and tumour cells. 2 In Cerebrospinal Fluid (CSF), a WBC count of >10 cells/µL in adults and >10-30 cells/µL in children suggests meningitis or encephalitis, 3 whereas in ascitic fluid spontaneous bacterial peritonitis is diagnosable when >250 Polymorphonuclear (PMNs) cells/µL are counted. Cytological analysis comprises of microscopic examination, wherein the total and differential cell count is performed using a haemocytometer.…”

“…Cytological analysis comprises of microscopic examination, wherein the total and differential cell count is performed using a haemocytometer. 2 In recent years, automated haematology analyzers with a Body Fluid Mode (BFM) are provided for the extension of its function in the analysis of cells in BFs. In this study, we evaluated the gold standard techniques of the improved Neubauer chamber,and the counting of cells using an automated analyser, the Sysmex XT-4000i with BFM to count WBCs (Differential).…”