Genome Mining of Endophytic<i>Streptomyces wadayamensis</i>Reveals High Antibiotic Production Capability

Angolini, Célio Fernando Figueiredo; Gonçalves, Ana B.; Sigrist, Renata; Paulo, Bruno S.; Samborskyy, Markiyan; Cruz, Pedro L. R.; Vivian, Adriana F.; Schmidt, Eduardo Morgado; Eberlin, Marcos N.; Araújo, Welington Luiz; Oliveira, Luciana G. de

doi:10.5935/0103-5053.20160180

Cited by 7 publications

(5 citation statements)

References 29 publications

(35 reference statements)

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“…XY006, an endophyte isolated from Tea ( Camellia sinensis ) had 27 possible secondary metabolite biosynthetic gene clusters encoding for antimycin, melanin, informatipeptin, albaflavenone, and roseoflavin (Vazquez-Hernandez et al, 2017 ). Genome mining of Streptomyces wadayamensis A23, an endophytic strain showed the presence of biosynthetic gene clusteres for antimycins and candicidin (Angolini et al, 2016 ).…”

Section: Biosynthetic Gene Clusters In Endophytic Actinobacteriamentioning

confidence: 99%

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

2018

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Actinobacteria are wide spread in nature and represent the largest taxonomic group within the domain Bacteria. They are abundant in soil and have been extensively explored for their therapeutic applications. This versatile group of bacteria has adapted to diverse ecological habitats, which has drawn considerable attention of the scientific community in recent times as it has opened up new possibilities for novel metabolites that may help in solving some of the most challenging problems of the day, for example, novel drugs for drug-resistant human pathogens, affordable means to maintain ecological balance in various habitats, and alternative practices for sustainable agriculture. Traditionally, free dwelling soil actinobacteria have been the subject of intensive research. Of late, symbiotic actinobacteria residing as endophytes within the plant tissues have generated immense interest as potential source of novel compounds, which may find applications in medicine, agriculture, and environment. In the light of these possibilities, this review focuses on the diversity of endophytic actinobacteria isolated from the plants of extreme habitats and specific ecological niches. Furthermore, an attempt has been made to assign chemical class to the compounds obtained from endophytic actinobacteria. Potential therapeutic applications of these compounds and the utility of endophytic actinobacteria in agriculture and environment are discussed.

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Section: Biosynthetic Gene Clusters In Endophytic Actinobacteriamentioning

confidence: 99%

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

2018

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“…Close fragmentation pattern was reported for desferrioxamine B complexed with Fe(III) (difference of 29 Da in the mass fragments due the difference of mass between Al and Fe) 53 . For compound 4, mass fragmentation yielded to fragments at m/z 423.2225 and 223.1101, this fragmentation pattern corresponds to the typical neutral losses of molecules of succinylcadaverine (200.1160 Da) 54 . Compounds and 4 were recovery using all types of extraction methods when GYE-ASW medium was used to bacterial growth.…”

Section: Five Bgcs Were Expressedmentioning

confidence: 91%

Genome Mining Reveals Secondary Metabolites of Antarctic Bacterium Streptomyces Albidoflavus ANT_B131 Related to Antimicrobial and Antiproliferative Activities

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et al. 2020

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The antimicrobial resistance requires the urgent mining of new antimicrobials. Actinobacteria, specially Streptomyces genus, are responsible for production of numerous clinical antibiotics and anticancer agents. The use of genome mining can reveal the genetic potential of a strain to produce natural products, however the potential may not be express under laboratory conditions. In the present study, the Antarctic bacterium was taxonomically affiliated as Streptomyces albidoflavus, ANT_B131. The crude extracts showed antimicrobial activity against fungi, Gram-positive and Gram-negative bacteria and antiproliferative activity against five human tumor cell lines. Whole genome sequencing reveals a 6.96 Mb genome size and the genome mining showed 24 BGCs, representing 13.3% of the genome. The use of three culture media and extraction methods reveal the expression and recovery of 20% of the BGCs. The natural products identified were surugamide A, surugamide D, desferrioxamine B + Al, desferrioxamine E, and ectoine. This study reveals the potential of S. albidoflavus ANT_B131 as a natural product producer. Yet, the diversity of culture media and extraction methods could enhance the BGCs expression and recovery of natural products, and could be a new strategy to intensify the BGC expression of natural products.

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“…Genome mining tools have made it more convenient to look for innovations in natural product discovery with majority of the bioactive compounds biosynthetic pathway of polyketides governed by a complex enzyme system, called polyketide synthase encoded by PKS gene cluster [121,122]. Available whole genome draft of endophytic actinobacteria also revealed the presence of PKS and NPRS genes suggesting that these microbes are the possible source for many novel bioactive compounds [123,124]. Screening for the presence of bioactive secondary metabolites in actinobacteria can be done using a high throughput method based on gene clusters.…”

Section: Genomic Mining and Omic Based Screening Of Rare Actinomycetesmentioning

confidence: 99%

Uncovering the biodiversity and biosynthetic potentials of rare actinomycetes

et al. 2022

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Background Antibiotic resistance is on the rise, and new antibiotic research has slowed in recent years, necessitating the discovery of possibly novel microbial resources capable of producing bioactive compounds. Microbial infections are gaining resistance to existing antibiotics, emphasizing the need for novel medicinal molecules to be discovered as soon as possible. Because the possibilities of isolating undiscovered actinomycetes strains have decreased, the quest for novel products has shifted to rare actinomycetes genera from regular environments or the identification of new species identified in unusual habitats. Main body of the abstract The non-streptomyces actinobacteria are known as rare actinomycetes that are extremely difficult to cultivate. Rare actinomycetes are known to produce a variety of secondary metabolites with varying medicinal value. In this review, we reported the diversity of rare actinomycetes in several habitat including soil, plants, aquatic environment, caves, insects and extreme environments. We also reported some isolation methods to easily recover rare Actinobacteria from various sources guided with some procedures to identify the rare Actinobacteria isolates. Finally, we reported the biosynthetic potential of rare actinomycetes and its role in the production of unique secondary metabolites that could be used in medicine, agriculture, and industry. These microbial resources will be of interest to humanity, as antibiotics, insecticides, anticancer, antioxidants, to mention but a few. Short conclusion Rare actinomycetes are increasingly being investigated for new medicinal compounds that could help to address existing human health challenges such as newly emerging infectious illnesses, antibiotic resistance, and metabolic disorders. The bioactive secondary metabolites from uncommon actinomycetes are the subject of this review, which focuses on their diversity in different habitats, isolation, identification and biosynthetic potentials.

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Genome Mining of EndophyticStreptomyces wadayamensisReveals High Antibiotic Production Capability

Cited by 7 publications

References 29 publications

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

Genome Mining Reveals Secondary Metabolites of Antarctic Bacterium Streptomyces Albidoflavus ANT_B131 Related to Antimicrobial and Antiproliferative Activities

Uncovering the biodiversity and biosynthetic potentials of rare actinomycetes

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