Fragmentation Reactions of Rhodamine B and 6G as Revealed by High Accuracy Orbitrap Tandem Mass Spectrometry

Ferreira, B.; Correa, Deleon Nascimento; Eberlin, Marcos N.; Vendramini, Pedro Henrique

doi:10.5935/0103-5053.20160156

Cited by 13 publications

(15 citation statements)

References 9 publications

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“…4 b). Consistent with previous studies 19 , 20 , the linearity of the precursor ions for dox and rhodamine were found at m/z 544 Da and 443 Da, respectively. We later used this LC/MS standard curves to extrapolate the quantitative capability using the spectral microscopy.…”

Section: Resultssupporting

confidence: 91%

Label-free spectral imaging to study drug distribution and metabolism in single living cells

Alshammari

Pala

Katzir

et al. 2021

Sci Rep

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During drug development, evaluation of drug and its metabolite is an essential process to understand drug activity, stability, toxicity and distribution. Liquid chromatography (LC) coupled with mass spectrometry (MS) has become the standard analytical tool for screening and identifying drug metabolites. Unlike LC/MS approach requiring liquifying the biological samples, we showed that spectral imaging (or spectral microscopy) could provide high-resolution images of doxorubicin (dox) and its metabolite doxorubicinol (dox’ol) in single living cells. Using this new method, we performed measurements without destroying the biological samples. We calculated the rate constant of dox translocating from extracellular moiety into the cell and the metabolism rate of dox to dox’ol in living cells. The translocation rate of dox into a single cell for spectral microscopy and LC/MS approaches was similar (~ 1.5 pM min−1 cell−1). When compared to spectral microscopy, the metabolism rate of dox was underestimated for about every 500 cells using LC/MS. The microscopy approach further showed that dox and dox’ol translocated to the nucleus at different rates of 0.8 and 0.3 pM min−1, respectively. LC/MS is not a practical approach to determine drug translocation from cytosol to nucleus. Using various methods, we confirmed that when combined with a high-resolution imaging, spectral characteristics of a molecule could be used as a powerful approach to analyze drug metabolism. We propose that spectral microscopy is a new method to study drug localization, translocation, transformation and identification with a resolution at a single cell level, while LC/MS is more appropriate for drug screening at an organ or tissue level.

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Section: Resultssupporting

confidence: 91%

Label-free spectral imaging to study drug distribution and metabolism in single living cells

Alshammari

Pala

Katzir

et al. 2021

Sci Rep

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“…S15 and S16. [65][66][67][68] In the case of MB, we identified the thionin intermediate (m/z = 228) after 100 min of reaction, suggesting that the MB degradation occurs via the auxochrome group degradation, i.e. via demethylation cleavage during the photo catalytic degradation 69,70 -Fig.…”

Section: Photocatalytic Activitymentioning

confidence: 93%

The use of N^N ligands as an alternative strategy for the sol–gel synthesis of visible-light activated titanias

et al. 2020

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“…To quantify the maximum and minimum mass spectrometry concentrations limits of the selected chemicals, Q-TOF MS and LC/MS was conducted. For Q-TOF MS method, Rhodamine was by injected at different concentrations (Table 2) and by checking the molecular mass the maximum concentration limit to detect at 5 nM (Fig 11), while 0.2 nM is the minimum concentration with precursor ion at m/z 443 Da which consistent with previous studies (Ferreira et al, 2017) (Fig 12).…”

Section: Q-tof Ms and Lc/mssupporting

confidence: 84%

Identification of Molecules by Spectral Imaging

Alshammari¹

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Identification of Molecules by Spectral Imaging by Qamar A. Alshammari Spectral imaging is a powerful technique which uses the wavelength to identify/quantify the exact location and amount of the molecules. It facilitates the identification of materials and studying their properties through analyzing the way they interact with light. The study of light interaction with elements is called spectroscopy; spectroscopy examines how light behaves in the target and recognizes materials based on their spectral signatures. Spectral signatures can be compared to fingerprints which can be used to identify a person; spectral signatures can be used to identify materials. Therefore, we hypothesize that identifying the exact location and quantity of molecules present in the given cells samples can be done by using a spectral imaging system. In this study, we identify the exact UV-Vis and fluorescence spectra of organic substances including Rhodamine 6G, Doxorubicin and UV-Vis spectra inorganic compounds including silver (Ag), gold (Au) nanoparticles (NPs). After that, we used the Q-TOF LC/MS system to quantify the maximum and minimum detectable concentrations of Rhodamine 6G and Doxorubicin by checking the chemicals spectrum based on the molecular weight. In addition, we used HPLC system to quantify the chemicals basing on their UV spectrum. Forwards, we used spectral imaging system to determine the exact amount and location of the molecules within cells samples. For Rhodamine 6G and doxorubicin, we started with the minimum detectable concentration by Q-TOF and consider it as a maximum limit with spectral imaging. And for NPs we used the maximum concentration for the analysis. Using the spectral imaging we were able to vii detect the exact location of Rhodamine 6G which was in the cytoplasm, Doxorubicin in the nucleoplasm, and NPs in both. Furthermore, spectral imaging was able to detect much lower concentrations of Rhodamine 6G and Doxorubicin by the spectrum in comparison to Q-TOF LC/MS. viii

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Fragmentation Reactions of Rhodamine B and 6G as Revealed by High Accuracy Orbitrap Tandem Mass Spectrometry

Cited by 13 publications

References 9 publications

Label-free spectral imaging to study drug distribution and metabolism in single living cells

Label-free spectral imaging to study drug distribution and metabolism in single living cells

The use of N^N ligands as an alternative strategy for the sol–gel synthesis of visible-light activated titanias

Identification of Molecules by Spectral Imaging

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