An Insight Into the Intraspecific Variation of Biosynthetic Gene Clusters Between Strains ofBurkholderia thailandensisspp.

Abstract

The present study aimed to investigate the intraspecific variation of biosynthetic gene clusters (BGCs) in different strains of Burkholderia thailandensis in order to guide natural products (NPs) discovery process. Species from the genus Burkholderia are emerging as promising species due to their biosynthetic potential. Through genome-mining strategies, it was able to identify that B. thailandensis strains present major genome variation between chromosomes I and II and the standard. The positioning of BGCs also differs when comparing each chromosome. Classical pathways as well as terpene and bacteriocins were commonly identified to all of them and BGCs related to the production of nonribosomal peptides and polyketides compounds are often noticed. In addition, hybrids BGCs were identified as using large amount of replicon information. Among all species studied, the strain MSMB121 showed greater potential for biosynthesizing novel natural products and after phylogenetic analysis, the likelihood of recognizing sites of novelties was assigned. Keywords: Burkholderia thailandensis, genome-mining, natural products discovery IntroductionSpecies of the genus Burkholderia have emerged as owing promising biosynthetic capability for diverse natural products (NPs). Recently, a remarkable study about the potential of microorganisms in biosynthesizing NPs pointed that proteobacteria species present large number of biosynthetic gene clusters (BGCs).1 This global analysis included species from the genus Pseudomonas spp. and Burkholderia spp. as containing the majority of BGCs counting for proteobacteria representatives. However, the great interest is related to Burkholderia spp., once Pseudomonas spp. are extensively studied. About the likelihood of producing novel NPs, Burkholderia genomes present, statistically, higher percentage of thiotemplate modular systems than those of bacilli, cyanobacteria, myxobacteria and fungi, and is only second to that of actinobacteria.2 These modular systems are related to the production of many classes of pharmaceutical compounds, including polyketide synthase (PKS)-and nonribosomal peptide synthase (NRPS)-related products. 3According to the German Collection of Microorganisms and Cell Cultures (DSMZ) database, the genus Burkholderia comprises more than 90 species. These species inhabit the most diverse types of ecological niches such as soil, water, rhizosphere and plant surface. 4NPs from Burkholderia spp. are structurally and functionally diverse, comprising benzoquinone; lactone; and polyene compounds, lasso peptides, nonribosomal peptides, statins, and polyketides. These compounds present important biological activities. In addition, some of these small molecules from Burkholderia sp. have entered as drug candidates to preclinical evaluation. 5Burkholderia thailandensis (B. thailandensis) E264 presented a high level of similarity to the BGC BTH-II0204-207 from Burkholderia pseudomallei (B. pseudomallei) K96243 related to the production of betulinan/terferol analogues.6 Experiments indicat...

A strategy for the identification of patterns in the biosynthesis of nonribosomal peptides by Betaproteobacteria species

“…In this sense, the wide occurrence of asp.ser and orn.ser and the elevated values of JI associated to species which present them, these POPs could be an indicative of biosynthetic patterns in species of BPB. As an example, species of the genus Burkholderia are associated to the production of NPs with the subunits orn.ser and asp.ser as portions of the entire molecule 44 .…”